Summary: Sodium fluoroacetate (1 m) caused an accumulation of citrate and altered the lipid composition in cells of sp. 795. It also inhibited acetylene reduction (nitrogen fixation) by the alga - markedly under aerobic conditions, but much less so in the absence of oxygen. This inhibition is largely the result of the conversion of fluoroacetate to fluorocitrate which, by inhibiting aconitate hydratase (EC, interrupts the synthesis of the 2-oxoglutarate required for the assimilation of NH . The consequent accumulation of NH within the cells of sp. inhibits nitrogenase synthesis and, since oxygen rapidly inactivates pre-existing nitrogenase, nitrogen fixation by sp. decreases under aerobic conditions.


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