RT Journal Article SR Electronic(1) A1 Gallon, John R. A1 Ul-Haque, M. Ikram A1 Chaplin, Alan E.YR 1978 T1 Fluoroacetate Metabolism in Gloeocapsa sp. LB795 and its Relationship to Acetylene Reduction (Nitrogen Fixation) JF Microbiology, VO 106 IS 2 SP 329 OP 336 DO https://doi.org/10.1099/00221287-106-2-329 PB Microbiology Society, SN 1465-2080, AB Sodium fluoroacetate (1 mM) caused an accumulation of citrate and altered the lipid composition in cells of Gloeocapsa sp. LB795. It also inhibited acetylene reduction (nitrogen fixation) by the alga - markedly under aerobic conditions, but much less so in the absence of oxygen. This inhibition is largely the result of the conversion of fluoroacetate to fluorocitrate which, by inhibiting aconitate hydratase (EC 4.2.1.3), interrupts the synthesis of the 2-oxoglutarate required for the assimilation of NH4 +. The consequent accumulation of NH4 + within the cells of Gloeocapsa sp. inhibits nitrogenase synthesis and, since oxygen rapidly inactivates pre-existing nitrogenase, nitrogen fixation by Gloeocapsa sp. decreases under aerobic conditions., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-106-2-329