SUMMARY: Using R68.45 as a conjugative plasmid in crosses between strains of and transfer of chromosomal genes occurred. Haploid recombinants were formed in which and alleles were transferred from to In crosses in the reverse direction haploid recombinants arose at barely detectable frequencies and progeny were isolated which were high frequency donors of the selected allele. Genetic evidence showed that such progeny harboured R-primes in which sections of the chromosome were inserted into R68.45. Four different classes of R68.45-prime, each carrying a different prototrophic marker, were obtained.


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