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Sporulation kinetics and spore heat resistance data were compared for a lysogenic strain of Clostridium perfringens, s9, before and after curing with ultraviolet irradiation. The cured strain showed the same growth rate in broth media as the lysogenic strain but took 6 h longer to form refractile spores. For lysogenized and cured strains the percentages of refractile spores produced that were heat-resistant (80 ° C for 15 min) were 50 and 0·2, respectively. When reinfected with the temperate phage, the cured strain produced spores in 2 to 3 h, like the original lysogenic culture, and 10 % of the spores produced were heat-resistant.
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