%0 Journal Article %A Stewart, Adelle W. %A Johnson, Michael G. %T Increased Numbers of Heat-resistant Spores Produced by Two Strains of Clostridium perfringens Bearing Temperate Phage s9 %D 1977 %J Microbiology, %V 103 %N 1 %P 45-50 %@ 1465-2080 %R https://doi.org/10.1099/00221287-103-1-45 %I Microbiology Society, %X Sporulation kinetics and spore heat resistance data were compared for a lysogenic strain of Clostridium perfringens, s9, before and after curing with ultraviolet irradiation. The cured strain showed the same growth rate in broth media as the lysogenic strain but took 6 h longer to form refractile spores. For lysogenized and cured strains the percentages of refractile spores produced that were heat-resistant (80 ° C for 15 min) were 50 and 0·2, respectively. When reinfected with the temperate phage, the cured strain produced spores in 2 to 3 h, like the original lysogenic culture, and 10 % of the spores produced were heat-resistant. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-103-1-45