SUMMARY: Certain single-gene acetate-requiring mutants of are deficient in their ability to oxidize pyruvate directly, but can decarboxylate pyruvate to acetaldehyde and can form ethanol. The growth characteristics of the mutants indicate that they can form acetate from glucose or ethanol. Mutants are inhibited by glucose or sucrose. This glucose inhibition is relieved by the single-gene suppressor mutations and which lower the activity of pyruvic carboxylase, decrease the amount of ethanol formed and permit some growth of and strains in absence of added acetate. The mutation also lowers ethanol dehydrogenase activity, but this activity can be partially restored by growth of strains in the presence of ethanol. As a result of the nearly complete block in their pyruvate metabolism, strains are forced to metabolize glucose by pathways which produce more of the initial respiratory CO from the C-1 carbon of glucose.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error