%0 Journal Article %A Strauss, B. S. %A Pierog, Sophie %T Gene Interactions: the Mode of Action of the Suppressor of Acetate-requirinǵ Mutants of Neurospora crassa %D 1954 %J Microbiology, %V 10 %N 2 %P 221-235 %@ 1465-2080 %R https://doi.org/10.1099/00221287-10-2-221 %I Microbiology Society, %X SUMMARY: Certain single-gene acetate-requiring mutants of Neurospora crassa (ac) are deficient in their ability to oxidize pyruvate directly, but can decarboxylate pyruvate to acetaldehyde and can form ethanol. The growth characteristics of the ac mutants indicate that they can form acetate from glucose or ethanol. ac Mutants are inhibited by glucose or sucrose. This glucose inhibition is relieved by the single-gene suppressor mutations sp and car which lower the activity of pyruvic carboxylase, decrease the amount of ethanol formed and permit some growth of ac sp and ac car strains in absence of added acetate. The sp mutation also lowers ethanol dehydrogenase activity, but this activity can be partially restored by growth of sp strains in the presence of ethanol. As a result of the nearly complete block in their pyruvate metabolism, ac sp strains are forced to metabolize glucose by pathways which produce more of the initial respiratory CO2 from the C-1 carbon of glucose. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-10-2-221