1887

Abstract

Recently, a real-time PCR (RT-PCR) assay based on sequence from the gene for pertactin was proposed for identification of . Here, it is reported that the pertactin gene sequence for the region that encompasses the RT-PCR probe and primers is nearly identical to that of many strains of human and avian origin. Additionally, it is demonstrated that such strains are erroneously identified as using the RT-PCR assay. These data suggest that the use of the assay without confirmatory testing may result in erroneous identification of a significant proportion of human isolates of as .

Keyword(s): RT-PCR, real-time PCR
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2007-12-01
2019-10-23
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References

  1. Diavatopoulos, D. A., Cummings, C. A., Schouls, L. M., Brinig, M. M., Relman, D. A. & Mooi, F. R. ( 2005; ). Bordetella pertussis, the causative agent of whooping cough, evolved from a distinct, human-associated lineage of B. bronchiseptica. PLoS Pathog 1, e45 [CrossRef]
    [Google Scholar]
  2. Diavatopoulos, D. A., Hijnen, M. & Mooi, F. R. ( 2006; ). Adaptive evolution of the Bordetella autotransporter pertactin. J Evol Biol 19, 1931–1938.[CrossRef]
    [Google Scholar]
  3. Register, K. B. ( 2004; ). Comparative sequence analysis of Bordetella bronchiseptica pertactin gene (prn) repeat region variants in swine vaccines and field isolates. Vaccine 23, 48–57.[CrossRef]
    [Google Scholar]
  4. Register, K. B. & Sanden, G. N. ( 2006; ). Prevalence and sequence variants of IS481 in Bordetella bronchiseptica: implications for IS481-based detection of Bordetella pertussis. J Clin Microbiol 44, 4577–4583.[CrossRef]
    [Google Scholar]
  5. Register, K. B., Boisvert, A. & Ackermann, M. R. ( 1997; ). Use of ribotyping to distinguish Bordetella bronchiseptica isolates. Int J Syst Bacteriol 47, 678–683.[CrossRef]
    [Google Scholar]
  6. Riffelmann, M., Wirsing von Konig, C. H., Caro, V. & Guiso, N. ( 2005; ). Nucleic acid amplification tests for diagnosis of Bordetella infections. J Clin Microbiol 43, 4925–4929.[CrossRef]
    [Google Scholar]
  7. van der Zee, A., Mooi, F., Van Embden, J. & Musser, J. ( 1997; ). Molecular evolution and host adaptation of Bordetella spp.: phylogenetic analysis using multilocus enzyme electrophoresis and typing with three insertion sequences. J Bacteriol 179, 6609–6617.
    [Google Scholar]
  8. Vincart, B., De Mendonca, R., Rottiers, S., Vermeulen, F., Struelens, M. J. & Denis, O. ( 2007; ). A specific real-time PCR assay for the detection of Bordetella pertussis. J Med Microbiol 56, 918–920.[CrossRef]
    [Google Scholar]
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vol. , part 12, pp. 1608–1610

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