Recently, a real-time PCR (RT-PCR) assay based on sequence from the gene for pertactin was proposed for identification of . Here, it is reported that the pertactin gene sequence for the region that encompasses the RT-PCR probe and primers is nearly identical to that of many strains of human and avian origin. Additionally, it is demonstrated that such strains are erroneously identified as using the RT-PCR assay. These data suggest that the use of the assay without confirmatory testing may result in erroneous identification of a significant proportion of human isolates of as .

Keyword(s): RT-PCR, real-time PCR

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