1887

Abstract

The resident bacterial flora of the large intestine has become increasingly recognized as an essential component in the pathogenesis of ulcerative colitis (UC). However, it is still not known whether the bacterial flora in general or certain bacterial species of the intestinal microbial flora contribute to the pathogenesis of the disease. In order to investigate the composition of the mucosa-associated microbial flora in UC, mucosal tissue samples from patients with active UC and from control subjects with non-inflammatory conditions were analysed and compared. To cover the whole spectrum of intestinal bacteria and to circumvent the known bias introduced by culture-based techniques, comparative 16S rRNA gene sequence analysis was used to determine the bacterial composition in the mucosal tissue samples. The investigation revealed an abundance of sequences from spp. and spp. in the mucosal tissue of patients with UC compared with individuals showing no signs of disease. The higher incidence of populations of members of the in UC suggests that these may have an influence on the pathogenesis of the disease.

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2006-05-01
2024-03-19
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References

  1. Amann R. I., Ludwig W., Schleifer K.-H. 1995; Phylogenetic identification and in situ detection of individual microbial cells without cultivation. Microbiol Rev 59:143–169
    [Google Scholar]
  2. Barcenilla A., Pryde S. E., Martin J. C., Duncan S. H., Stewart C. S., Henderson C., Flint H. J. 2000; Phylogenetic relationships of butyrate-producing bacteria from the human gut. Appl Environ Microbiol 66:1654–1661 [CrossRef]
    [Google Scholar]
  3. Berg R. D. 1996; The indigenous gastrointestinal microflora. Trends Microbiol 4:430–435 [CrossRef]
    [Google Scholar]
  4. Blumberg R. S., Saubermann L. J., Strober W. 1999; Animal models of mucosal inflammation and their relation to human inflammatory bowel disease. Curr Opin Immunol 11:648–656 [CrossRef]
    [Google Scholar]
  5. Breeling J. L., Onderdonk A. B., Cisneros R. L., Kasper D. L. 1988; Bacteroides vulgatus outer membrane antigens associated with carrageenan-induced colitis in guinea pigs. Infect Immun 56:1754–1759
    [Google Scholar]
  6. Campirei M., Gionchetti P. 2001; Bacteria as the cause of ulcerative colitis. Gut 48:132–135 [CrossRef]
    [Google Scholar]
  7. Chapman R. W., Selby W. S., Jewell D. P. 1986; Controlled trial of intravenous metronidazole as an adjunct to corticosteroids in severe ulcerative colitis. Gut 27:1210–1212 [CrossRef]
    [Google Scholar]
  8. Dianda L., Hanby A. M., Wright N. A., Sebesteny A., Hayday A. C., Owen M. J. 1997; T cell receptor- αβ -deficient mice fail to develop colitis in the absence of a microbial environment. Am J Pathol 150:91–97
    [Google Scholar]
  9. Eckburg P. B., Bik E. M., Bernstein C. N., Purdom E., Dethlefsen L., Sargent M., Gill S. R., Nelson K. E., Relman D. A. 2005; Diversity of the human intestinal microbial flora. Science 308:1635–1638 [CrossRef]
    [Google Scholar]
  10. Ell C., Fischbach W., Keller R., Dehe M., Mayer G., Schneider B., Albrecht U., Schuette W. Hintertux Study Group 2003; A randomized, blinded, prospective trial to compare the safety and efficacy of three bowel-cleansing solutions for colonoscopy (HSG-01*). Endoscopy 35:300–304 [CrossRef]
    [Google Scholar]
  11. Fenner L., Roux V., Mallet M.-N., Raoult D. 2005; Bacteroides massiliensis sp. nov., isolated from blood culture of a newborn. Int J Syst Evol Microbiol 55:1335–1337 [CrossRef]
    [Google Scholar]
  12. Fiocchi C. 1998; Inflammatory bowel disease: etiology and pathogenesis. Gastroenterology 115:182–205 [CrossRef]
    [Google Scholar]
  13. Garcia-Lafuente A., Antolin M., Guarner F. & 8 other authors; 1997; Incrimination of anaerobic bacteria in the induction of experimental colitis. Am J Physiol 272:G10–G15
    [Google Scholar]
  14. Greenbloom S. L., Steinhart A. H., Greenberg G. R. 1998; Combination ciprofloxacin and metronidazole for active Crohn's disease. Can J Gastroenterol 12:53–56
    [Google Scholar]
  15. Hartley M. G., Hudson M. J., Swarbrick E. T., Hill M. J., Gent A. E., Hellier M. D., Grace R. H. 1992; The rectal mucosa-associated microflora in patients with ulcerative colitis. J Med Microbiol 36:96–103 [CrossRef]
    [Google Scholar]
  16. Hold G. L., Pryde S. E., Russell V. J., Furrie E., Flint H. J. 2002; Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis. FEMS Microbiol Ecol 39:33–39 [CrossRef]
    [Google Scholar]
  17. Hooper L. V., Gordon J. I. 2001; Commensal host–bacterial relationships in the gut. Science 292:1115–1118 [CrossRef]
    [Google Scholar]
  18. Hooper L. V., Wong M. H., Thelin A., Hansson L., Falk P. G., Gordon J. I. 2001; Molecular analysis of commensal host–microbial relationships in the intestine. Science 291:881–884 [CrossRef]
    [Google Scholar]
  19. Leser T. D., Amenuvor J. Z., Jensen T. K., Lindecrona R. H., Boye M., Møller K. 2002; Culture-independent analysis of gut bacteria: the pig gastrointestinal tract microbiota revisited. Appl Environ Microbiol 68:673–690 [CrossRef]
    [Google Scholar]
  20. Macpherson A., Khoo U. Y., Forgacs I., Philpott-Howard J., Bjarnason I. 1996; Mucosal antibodies in inflammatory bowel disease are directed against intestinal bacteria. Gut 38:365–375 [CrossRef]
    [Google Scholar]
  21. Manz W., Amann R., Ludwig W., Schleifer K.-H. 1996; Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum Cytophaga–Flavobacter–Bacteroides in the natural environment. Microbiology 142:1097–1106 [CrossRef]
    [Google Scholar]
  22. Marchesi J. R., Sato T., Weightman A. J., Martin T. A., Fry J. C., Hiom S. J., Wade W. G. 1998; Design and evaluation of useful bacterium-specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64:795–799
    [Google Scholar]
  23. Marks C. G., Hawley P. R., Peach S. L., Drasar B. S., Hill M. J. 1979; The effects of phthalylsulphathiazole on the bacteria of the colonic mucosa and intestinal contents as revealed by the examination of surgical samples. Scand J Gastroenterol 14:891–896 [CrossRef]
    [Google Scholar]
  24. Mishina D., Katsel P., Brown S. T., Gilberts E. C. A. M., Greenstein R. J. 1996; On the etiology of Crohns disease. Proc Natl Acad Sci U S A 93:9816–9820 [CrossRef]
    [Google Scholar]
  25. Onderdonk A. B., Hermos J. A., Dzink J. L., Bartlett J. G. 1978; Protective effect of metronidazole in experimental ulcerative colitis. Gastroenterology 74:521–526
    [Google Scholar]
  26. Onderdonk A. B., Cisneros R. L., Bronson R. T. 1983; Enhancement of experimental ulcerative colitis by immunization with Bacteroides vulgatus . Infect Immun 42:783–788
    [Google Scholar]
  27. Ott S. J., Musfeldt M., Wenderoth D. F., Hampe J., Brant O., Fölsch U. R., Timmis K. N., Schreiber S. 2004; Reduction in diversity of the colonic mucosa associated bacterial microflora in patients with active inflammatory bowel disease. Gut 53:685–693 [CrossRef]
    [Google Scholar]
  28. Papadakis K. A., Targan S. R. 1999; Current theories on the causes of inflammatory bowel disease. Gastroenterol Clin North Am 28:283–296
    [Google Scholar]
  29. Prantera C., Berto E., Scribano M. L., Falasco G. 1998; Use of antibiotics in the treatment of active Crohn's disease: experience with metronidazole and ciprofloxacin. Ital J Gastroenterol Hepatol 30:602–606
    [Google Scholar]
  30. Pryde S. E., Richardson A. J., Stewart C. S., Flint H. J. 1999; Molecular analysis of the microbial diversity present in the colonic wall, colonic lumen and caecal lumen of a pig. Appl Environ Microbiol 65:5372–5377
    [Google Scholar]
  31. Rath H. C., Herfarth H. H., Ikeda J. S. & 7 other authors; 1996; Normal luminal bacteria, especially Bacteroides species, mediate chronic colitis, gastritis, and arthritis in HLA-B27/human β 2 microglobulin transgenic rats. J Clin Invest 98:945–953 [CrossRef]
    [Google Scholar]
  32. Rath H. C., Ikeda J. S., Linde H. J., Schölmerich J., Wilson K. H., Sartor R. B. 1999; Varying cecal bacterial loads influences colitis and gastritis in HLA-B27 transgenic rats. Gastroenterology 116:310–319 [CrossRef]
    [Google Scholar]
  33. Ruseler-van Embden J. G., Both-Patoir H. C. 1983; Anaerobic Gram-negative faecal flora in patients with Crohn's disease and healthy subjects. Antonie van Leeuwenhoek 49:125–132 [CrossRef]
    [Google Scholar]
  34. Rutgeerts P., Hiele M., Geboes K., Peeters M., Penninckx F., Arets R., Kerremans R. 1995; Controlled trial of metronidazole treatment for prevention of Crohn's recurrence after ileal resection. Gastroenterology 108:1617–1621 [CrossRef]
    [Google Scholar]
  35. Salzman N. H., de Jong H., Paterson Y., Harmsen H. J. M., Welling G. W., Bos N. A. 2002; Analysis of 16S libraries of mouse gastrointestinal microflora reveals a large new group of mouse intestinal bacteria. Microbiology 148:3651–3660
    [Google Scholar]
  36. Schuppler M., Mertens F., Schön G., Göbel U. B. 1995; Molecular characterization of nocardioform actinomycetes in activated sludge by 16S rRNA analysis. Microbiology 141:513–521 [CrossRef]
    [Google Scholar]
  37. Schuppler M., Lötzsch K., Waidmann M., Autenrieth I. B. 2004; An abundance of Escherichia coli is harbored by the mucosa-associated flora of interleukin-2-deficient mice. Infect Immun 72:1983–1990 [CrossRef]
    [Google Scholar]
  38. Seksik P., Rigottier-Gois L., Gramet G., Sutren M., Pochart P., Marteau P., Rian R., Doré J. 2003; Alterations of the dominant faecal bacterial groups in patients with Crohn's disease of the colon. Gut 52:237–242 [CrossRef]
    [Google Scholar]
  39. Sellon R. K., Tonkonogy S., Schultz M., Dielemann L. A., Grenther W., Ballish E., Rennick D. M., Sartor R. B. 1998; Resident enteric bacteria are necessary for development of spontaneous colitis and immune system activation in interleukin-10-deficient mice. Infect Immun 66:5224–5231
    [Google Scholar]
  40. Suau A., Bonnet R., Sutren M., Godon J.-J., Gibson G. R., Collins M. D., Doré J. 1999; Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 65:4799–4807
    [Google Scholar]
  41. Swidsinski A., Ladhof A., Pernthaler A. & 8 other authors; 2002; Mucosal flora in inflammatory bowel disease. Gastroenterology 122:44–54 [CrossRef]
    [Google Scholar]
  42. Tsai H. H., Sunderland D., Gibson G. R., Hart C. A., Rhodes J. M. 1992; A novel mucin sulphatase from human faeces: its identification, purification and characterization. Clin Sci 82:447–454
    [Google Scholar]
  43. Tsai H. H., Dwarakanath A. D., Hart C. A., Milton J. D., Rhodes J. M. 1995; Increased faecal mucin sulphatase activity in ulcerative colitis: a potential target for treatment. Gut 36:570–576 [CrossRef]
    [Google Scholar]
  44. van Kruiningen H. J. 1995; On the use of antibiotics in Crohn's disease. J Clin Gastroenterol 20:310–316 [CrossRef]
    [Google Scholar]
  45. Waidmann M., Bechtold O., Frick J. S., Lehr H. A., Schubert S., Dobrindt U., Loeffler J., Bohn E., Autenrieth I. B. 2003; Bacteroides vulgatus protects against Escherichia coli -induced colitis in gnotobiotic interleukin-2-deficient mice. Gastroenterology 125:162–177 [CrossRef]
    [Google Scholar]
  46. Wang X., Heazlewood S. P., Krause D. O., Florin T. H. J. 2003; Molecular characterization of the microbial species that colonize human ileal and colonic mucosa by using 16S rDNA sequence analysis. J Appl Microbiol 95:508–520 [CrossRef]
    [Google Scholar]
  47. Wright D. P., Rosendale D. I., Robertson A. M. 2000; Prevotella enzymes involved in mucin oligosaccharide degradation and evidence for a small operon of genes expressed during growth on mucin. FEMS Microbiol Lett 190:73–79 [CrossRef]
    [Google Scholar]
  48. Zoetendal E. G., von Wright A., Vilpponen-Salmela T., Ben-Amor K., Akkermans A. D. L., de Vos W. M. 2002; Mucosa-associated bacteria in the human gastrointestinal tract are uniformly distributed along the colon and differ from the community recovered from feces. Appl Environ Microbiol 68:3401–3407 [CrossRef]
    [Google Scholar]
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