1887

Abstract

A simple method to detect mutations in the genome of that confer resistance to key drugs for leprosy was exploited on the basis of a reverse hybridization system. A series of oligonucleotide probes corresponding to each mutation in the , and genes for dapsone, rifampicin and ofloxacin resistance, respectively, were selected and fixed on a glass slide as capture probes, to develop a DNA microarray termed the leprosy drug susceptibility-DNA microarray (LDS-DA). Mutations in clinical isolates of were successfully identified by the LDS-DA. Feasibility studies were conducted to evaluate the performance of the LDS-DA in two developing countries, Myanmar and the Philippines. The high concordance of the results obtained by this method with the results of nucleotide sequencing strongly supports the applicability of the LDS-DA as a drug susceptibility test in place of sequencing, a time-consuming and costly procedure. This is a rapid and simple method for the simultaneous susceptibility testing of three front-line drugs for leprosy, and solves the problems of previously reported methods.

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2008-10-01
2019-11-19
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References

  1. Cambau, E., Perani, E., Guillemin, I., Jamet, P. & Ji, B. ( 1997; ). Multidrug rtesistance to dapsone, rifampicin, and ofloxacin in Mycobacterium leprae. Lancet 349, 103–104.
    [Google Scholar]
  2. Cambau, E., Bonnafous, P., Perani, E., Sougakoff, W., Ji, B. & Jarlier, V. ( 2001; ). Molecular detection of rifampicin and ofloxacin resistance for patients who experience relapse of multibacillary leprosy. Clin Infect Dis 34, 39–45.
    [Google Scholar]
  3. Cambau, E., Carthagena, L., Chauffour, A., Ji, B. & Jarlier, V. ( 2006; ). Dihydropteroate synthase mutations in the folP1 gene predict dapsone resistance in relapsed cases of leprosy. Clin Infect Dis 42, 238–241.[CrossRef]
    [Google Scholar]
  4. de Wit, M. Y. L., Faber, W. R. S., Krieg, R., Douglas, J. T., Lucas, S. B., Montreewasuwat, N., Pattyn, S. R. N., Hussain, R., Ponnighaus, J. M. & other authors ( 1991; ). Application of polymerase chain reaction for the detection of Mycobacterium leprae in skin tissue. J Clin Microbiol 29, 906–910.
    [Google Scholar]
  5. Honoré, N. & Cole, S. T. ( 1993; ). Molecular basis of rifampicin resistance in Mycobacterium leprae. Antimicrob Agents Chemother 37, 414–418.[CrossRef]
    [Google Scholar]
  6. Honoré, N., Perani, E., Telenti, A., Grosset, J. & Cole, S. T. ( 1993; ). A simple and rapid technique for the detection of rifampicin resistance in Mycobacterium leprae. Int J Lepr Other Mycobact Dis 61, 600–604.
    [Google Scholar]
  7. Ji, B. ( 2002; ). Rifampicin resistant leprosy: a review and research proposal of a pilot study. Lepr Rev 73, 2–8.
    [Google Scholar]
  8. Kai, M., Matsuoka, M., Nakata, N., Maeda, S., Gidoh, M., Maeda, Y., Hashimoto, K., Kobayashi, K. & Kashiwabara, Y. ( 1999; ). Diaminodiphenilsulfone resistance of Mycobacterium leprae due to mutations in the dihydropteroate synthase gene. FEMS Microbiol Lett 177, 231–235.[CrossRef]
    [Google Scholar]
  9. Lee, S. B., Kim, S. K., Kang, T. J., Chae, G. T., Chun, J. H., Shin, H. K., Kim, J. P., Ko, Y. H. & Kim, N. H. ( 2001; ). The prevalence of folP1 mutations associated with clinical resistance to dapsone, in Mycoabcterium leprae isolates from South Korea. Ann Trop Med Parasitol 95, 429–432.[CrossRef]
    [Google Scholar]
  10. Maeda, S., Matsuoka, M., Nakata, N., Kai, M., Maeda, Y., Hashimoto, K., Kimura, H., Kobayashi, K. & Kashiwabara, Y. ( 2001; ). Multidrug resistant Mycobacterium leprae from patients with leprosy. Antimicrob Agents Chemother 45, 3635–3639.[CrossRef]
    [Google Scholar]
  11. Matsuoka, M., Kashiwabara, Y. & Namisato, Y. ( 2000; ). A Mycobacterium leprae isolate resistant to dapsone, rifampicin, ofloxacine and sparfloxacin. Int J Lepr Other Mycobact Dis 68, 452–455.
    [Google Scholar]
  12. Matsuoka, M., Kashiwabara, Y., Zhang, L., Gotoh, M. & Kitajima, S. ( 2003; ). A second case of multidrug resistant Mycobacterium leprae isolated from a Japanese patient with relapsed lepromatous leprosy. Int J Lepr Other Mycobact Dis 71, 240–243.[CrossRef]
    [Google Scholar]
  13. Matsuoka, M., Zhang, L., Morris, M. F., Legua, P. & Wiens, C. ( 2005; ). Polymorphism in the rpoT gene in Mycobacterium leprae isolates obtained from Latin American countries and its possible correlation with the spread of leprosy. FEMS Microbiol Lett 243, 311–315.[CrossRef]
    [Google Scholar]
  14. Matsuoka, M., Teky, B., Khin, S. A., Kyaw, K., Tan, E. V., dela Cruz, E., Gelber, R., Saunderson, P., Balagon, M. A. & Pannikar, V. ( 2007; ). The frequency of drug resistance mutations in Mycobacterium leprae isolates in untreated and relapsed leprosy patients from Myanmar, Indonesia and the Philippines. Lepr Rev 78, 343–352.
    [Google Scholar]
  15. Rossau, R., Traore, H., de Beenhouwer, H., Mijs, W., Jannes, G., De Rijk, P. & Portaels, F. ( 1997; ). Evaluation of the INNO-LiPA Rif. TB assay, a reverse hybridization assay for the simultaneous detection of Mycobacterium tuberculosis complex and its resistance to rifampicin. Antimicrob Agents Chemother 41, 2093–2098.
    [Google Scholar]
  16. Sapkota, B. R., Ranjit, C., Neupane, K. D. & Macdonald, M. ( 2008; ). Developing and evaluation of a novel multiple-primer PCR amplification refractory mutation system for the rapid detection of mutations conferring rifampicin resistance in codon 425 of the rpoB gene of Mycobacterium leprae. J Med Microbiol 57, 179–184.[CrossRef]
    [Google Scholar]
  17. Shepard, C. ( 1960; ). The experimental disease that follows the injection of human leprosy bacillus into footpads of mice. J Exp Med 112, 445–454.[CrossRef]
    [Google Scholar]
  18. WHO ( 1998; ). WHO Expert Committee on Leprosy, seventh report. Technical report series 87. Geneva: World Health Organization.
  19. Williams, D. L. & Gillis, T. P. ( 2004; ). Molecular detection of resistance in Mycobacterium leprae. Lepr Rev 75, 118–130.
    [Google Scholar]
  20. Williams, D. L., Waguespack, C., Eisenack, K., Crawford, J. T., Portaels, F., Salfinger, M., Nolan, M. C., Abe, C., Stich-Groh, V. & Gillis, T. P. ( 1994; ). Characterization of rifampicin resistance in pathogenic mycobacteria. Antimicrob Agents Chemother 38, 2380–2386.[CrossRef]
    [Google Scholar]
  21. Williams, D. L., Spring, L., Harris, E., Roche, P. & Gillis, T. P. ( 2000; ). Dihydropteroate synthase of Mycobacterium leprae and dapsone resistance. Antimicrob Agents Chemother 44, 1530–1537.[CrossRef]
    [Google Scholar]
  22. Williams, D. L., Pittman, T. L., Gillis, T. P., Matsuoka, M. & Kashiwabara, Y. ( 2001; ). Simultaneous detection of Mycobacterium leprae and its susceptibility to dapsone using DNA heteroduplex analysis. J Clin Microbiol 39, 2083–2088.[CrossRef]
    [Google Scholar]
  23. Zhang, L., Namisato, M. & Matsuoka, M. ( 2004; ). A mutation at codon 516 in the rpoB gene of Mycobacterium leprae confers resistance to rifampicin. Int J Lepr Other Mycobact Dis 72, 468–472.[CrossRef]
    [Google Scholar]
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