@article{mbs:/content/journal/jmm/10.1099/jmm.0.2008/002600-0, author = "Matsuoka, Masanori and Aye, Khin Saw and Kyaw, Kyaw and Tan, Esterlina Virtudes and Balagon, Ma Victoria and Saunderson, Paul and Gelber, Robert and Makino, Masanao and Nakajima, Chie and Suzuki, Yasuhiko", title = "A novel method for simple detection of mutations conferring drug resistance in Mycobacterium leprae, based on a DNA microarray, and its applicability in developing countries", journal= "Journal of Medical Microbiology", year = "2008", volume = "57", number = "10", pages = "1213-1219", doi = "https://doi.org/10.1099/jmm.0.2008/002600-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.2008/002600-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", keywords = "LDS-DA, leprosy drug susceptibility-DNA microarray", keywords = "MDT, multidrug therapy", keywords = "BI, bacterial index", keywords = "DRDR, drug-resistance-determining region", abstract = "A simple method to detect mutations in the genome of Mycobacterium leprae that confer resistance to key drugs for leprosy was exploited on the basis of a reverse hybridization system. A series of oligonucleotide probes corresponding to each mutation in the folP1, rpoB and gyrA genes for dapsone, rifampicin and ofloxacin resistance, respectively, were selected and fixed on a glass slide as capture probes, to develop a DNA microarray termed the leprosy drug susceptibility-DNA microarray (LDS-DA). Mutations in clinical isolates of M. leprae were successfully identified by the LDS-DA. Feasibility studies were conducted to evaluate the performance of the LDS-DA in two developing countries, Myanmar and the Philippines. The high concordance of the results obtained by this method with the results of nucleotide sequencing strongly supports the applicability of the LDS-DA as a drug susceptibility test in place of sequencing, a time-consuming and costly procedure. This is a rapid and simple method for the simultaneous susceptibility testing of three front-line drugs for leprosy, and solves the problems of previously reported methods.", }