1887

Abstract

The aim of this study was to compare the performance of serological versus molecular typing methods to detect capsular polysaccharide (CP) and surface-associated polysaccharide antigen 336 phenotypes of isolates. Molecular typing of CP types 1, 5 and 8 was carried out using PCR, whereas serological typing of CP1, 2, 5, 8 and antigen 336 was carried out by slide agglutination using specific antisera. By genotyping, 14/31 strains were CP8 positive, 12/31 strains were CP5 and the remaining 6/31 isolates were non-typable (NT). One isolate was positive for both CP5 and CP8 by PCR, but was confirmed as CP8 type serologically. Detection of CP2 and type 336 by PCR was not possible because specific primers were either not available or non-specific. Using serotyping, 14/31 strains were CP8 positive, 11/31 CP5 positive and 2/31 positive for antigen 336. The remaining four isolates were serologically NT. However, three of four NT and two 336-positive isolates were encapsulated as determined by light microscopy after capsular staining. This discovery was surprising and warrants further investigations on the identification and characterization of additional capsular phenotypes prevalent among clinical isolates. It was concluded that serological typing was a better method than molecular typing for use in epidemiological investigations based upon the distribution of surface-associated polysaccharide antigens-based phenotypes.

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2014-11-01
2019-11-12
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References

  1. Babra C., Tiwari J., Costantino P., Sunagar R., Isloor S., Hegde N., Mukkur T.. ( 2014;). Human methicillin-sensitive Staphylococcus aureus biofilms: potential associations with antibiotic resistance persistence and surface polysaccharide antigens. . J Basic Microbiol 54:, 721–728. [CrossRef][PubMed]
    [Google Scholar]
  2. Cocchiaro J. L., Gomez M. I., Risley A., Solinga R., Sordelli D. O., Lee J. C.. ( 2006;). Molecular characterization of the capsule locus from non-typeable Staphylococcus aureus. . Mol Microbiol 59:, 948–960. [CrossRef][PubMed]
    [Google Scholar]
  3. Daum R. S., Spellberg B.. ( 2012;). Progress toward a Staphylococcus aureus vaccine. . Clin Infect Dis 54:, 560–567. [CrossRef][PubMed]
    [Google Scholar]
  4. Engelkirk P. G., Duben-Engelkirk J. L.. ( 2008;). Laboratory Diagnosis of Infectious Diseases: Essentials of Diagnostic Microbiology. Baltimore, MD:: Wolters Kluwer Health/Lippincott Williams & Wilkins;.
    [Google Scholar]
  5. Fattom A. I., Sarwar J., Basham L., Ennifar S., Naso R.. ( 1998;). Antigenic determinants of Staphylococcus aureus type 5 and type 8 capsular polysaccharide vaccines. . Infect Immun 66:, 4588–4592.[PubMed]
    [Google Scholar]
  6. Foster T. J.. ( 2004;). The Staphylococcus aureus “superbug”. . J Clin Invest 114:, 1693–1696. [CrossRef][PubMed]
    [Google Scholar]
  7. Karakawa W. W., Sutton A., Schneerson R., Karpas A., Vann W. F.. ( 1988;). Capsular antibodies induce type-specific phagocytosis of capsulated Staphylococcus aureus by human polymorphonuclear leukocytes. . Infect Immun 56:, 1090–1095.[PubMed]
    [Google Scholar]
  8. Maneval W. E.. ( 1941;). Staining bacteria and yeasts with acid dyes. . Biotech Histochem 16:, 13–19. [CrossRef]
    [Google Scholar]
  9. Nanra J. S., Buitrago S. M., Crawford S., Ng J., Fink P. S., Hawkins J., Scully I. L., McNeil L. K., Aste-Amézaga J. M.. & other authors ( 2012;). Capsular polysaccharides are an important immune evasion mechanism for Staphylococcus aureus. . Hum Vaccin Immunother 9:, 480–487. [CrossRef][PubMed]
    [Google Scholar]
  10. O’Riordan K., Lee J. C.. ( 2004;). Staphylococcus aureus capsular polysaccharides. . Clin Microbiol Rev 17:, 218–234. [CrossRef][PubMed]
    [Google Scholar]
  11. Ohlsen K., Lorenz U.. ( 2010;). Immunotherapeutic strategies to combat staphylococcal infections. . Int J Med Microbiol 300:, 402–410. [CrossRef][PubMed]
    [Google Scholar]
  12. Pozzi C., Wilk K., Lee J. C., Gening M., Nifantiev N., Pier G. B.. ( 2012;). Opsonic and protective properties of antibodies raised to conjugate vaccines targeting six Staphylococcus aureus antigens. . PLoS ONE 7:, e46648. [CrossRef][PubMed]
    [Google Scholar]
  13. Robbins J. B., Schneerson R., Horwith G., Naso R., Fattom A.. ( 2004;). Staphylococcus aureus types 5 and 8 capsular polysaccharide-protein conjugate vaccines. . Am Heart J 147:, 593–598. [CrossRef][PubMed]
    [Google Scholar]
  14. Roghmann M., Taylor K. L., Gupte A., Zhan M., Johnson J. A., Cross A., Edelman R., Fattom A. I.. ( 2005;). Epidemiology of capsular and surface polysaccharide in Staphylococcus aureus infections complicated by bacteraemia. . J Hosp Infect 59:, 27–32. [CrossRef][PubMed]
    [Google Scholar]
  15. Skurnik D., Merighi M., Grout M., Gadjeva M., Maira-Litran T., Ericsson M., Goldmann D. A., Huang S. S., Datta R.. & other authors ( 2010;). Animal and human antibodies to distinct Staphylococcus aureus antigens mutually neutralize opsonic killing and protection in mice. . J Clin Invest 120:, 3220–3233. [CrossRef][PubMed]
    [Google Scholar]
  16. Sompolinsky D., Samra Z., Karakawa W. W., Vann W. F., Schneerson R., Malik Z.. ( 1985;). Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. . J Clin Microbiol 22:, 828–834.[PubMed]
    [Google Scholar]
  17. Sutter D. E., Summers A. M., Keys C. E., Taylor K. L., Frasch C. E., Braun L. E., Fattom A. I., Bash M. C.. ( 2011;). Capsular serotype of Staphylococcus aureus in the era of community-acquired MRSA. . FEMS Immunol Med Microbiol 63:, 16–24. [CrossRef][PubMed]
    [Google Scholar]
  18. Tzianabos A. O., Wang J. Y., Lee J. C.. ( 2001;). Structural rationale for the modulation of abscess formation by Staphylococcus aureus capsular polysaccharides. . Proc Natl Acad Sci U S A 98:, 9365–9370. [CrossRef][PubMed]
    [Google Scholar]
  19. Verdier I., Durand G., Bes M., Taylor K. L., Lina G., Vandenesch F., Fattom A. I., Etienne J.. ( 2007;). Identification of the capsular polysaccharides in Staphylococcus aureus clinical isolates by PCR and agglutination tests. . J Clin Microbiol 45:, 725–729. [CrossRef][PubMed]
    [Google Scholar]
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