1887

Abstract

Antibiotic resistance in is a major concern worldwide. However, it is unclear whether resistance is associated with only a few highly prevalent clones or numerous and diverse clones. We monitored 349 healthy children and obtained nasopharyngeal cultures at five time points coinciding with health check-ups (4, 7, 10, 18 and 36 months) between 2008 and 2012. A total of 497 isolates from 257 healthy children were characterized using capsular serotyping, multilocus sequence typing and antibiotic resistance genotyping (, and mutations). Among these isolates, 25 serotypes and 66 sequence types (STs) were found, including 24 new STs with 11 new alleles. Although resistance was present in a variety of ST clones, most of the clones (57/66, 86.4 %) had one specific resistant or susceptible genotype. Of 233 phenotypically penicillin-non-susceptible isolates, 196 (84.1 %) belonged to only six clones, comprising ST90, ST236, ST242, ST3787, ST1437 and ST338 and their variants. We concluded that drug-resistant is associated with a limited number of highly prevalent clones that are capable of adapting to the community setting. Furthermore, we analysed the capsular gene evolution in serogroup 6. The strain ST2924 was probably the result of recombination of a 3563 bp fragment of the capsule locus acquired by an ST2924 strain from an ST90 or ST2924 strain. Compared with previous studies, our results showed a different recombination site ( and ) and a different profile (8-7-11), indicating that serogroup 6 strains have multiple sites for recombination as a mechanism of vaccine escape.

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2013-12-01
2019-10-22
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