RT Journal Article SR Electronic(1) A1 Otsuka, Taketo A1 Chang, Bin A1 Wada, Akihito A1 Okazaki, MinoruYR 2013 T1 Molecular epidemiology and serogroup 6 capsular gene evolution of pneumococcal carriage in a Japanese birth cohort study JF Journal of Medical Microbiology, VO 62 IS 12 SP 1868 OP 1875 DO https://doi.org/10.1099/jmm.0.066316-0 PB Microbiology Society, SN 1473-5644, AB Antibiotic resistance in Streptococcus pneumoniae is a major concern worldwide. However, it is unclear whether resistance is associated with only a few highly prevalent clones or numerous and diverse clones. We monitored 349 healthy children and obtained nasopharyngeal cultures at five time points coinciding with health check-ups (4, 7, 10, 18 and 36 months) between 2008 and 2012. A total of 497 S. pneumoniae isolates from 257 healthy children were characterized using capsular serotyping, multilocus sequence typing and antibiotic resistance genotyping (ermB, mefA/E and pbp mutations). Among these isolates, 25 serotypes and 66 sequence types (STs) were found, including 24 new STs with 11 new alleles. Although resistance was present in a variety of ST clones, most of the clones (57/66, 86.4 %) had one specific resistant or susceptible genotype. Of 233 phenotypically penicillin-non-susceptible isolates, 196 (84.1 %) belonged to only six clones, comprising ST906B, ST23619F, ST24223F, ST37876A, ST143723F and ST33823A and their variants. We concluded that drug-resistant S. pneumoniae is associated with a limited number of highly prevalent clones that are capable of adapting to the community setting. Furthermore, we analysed the capsular gene evolution in serogroup 6. The strain ST29246D was probably the result of recombination of a 3563 bp fragment of the capsule locus acquired by an ST29246C strain from an ST906B or ST29246B strain. Compared with previous studies, our results showed a different recombination site (wciN and wzx) and a different cps profile (8-7-11), indicating that serogroup 6 strains have multiple sites for cps recombination as a mechanism of vaccine escape., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.066316-0