1887

Abstract

A nested PCR assay (TPILC-PCR) was developed to detect and distinguish between assemblages A and B from human faeces by analysis of the triose phosphate isomerase gene (). The assay comprised an initial multiplexed block-based amplification. This was followed by two separate real-time PCR assays specific for assemblages A and B using a LightCycler and SYBR Green I to identify PCR products by melting-point analysis. RFLP analysis was applied to distinguish assemblage A groups I and II. The real-time nested PCR was evaluated using DNA extracted from purified giardial trophozoites, oocysts, whole faeces containing a range of potential pathogens (including ), faecal smears and bacterial suspensions. The assay was specific, sensitive, reproducible and rapid.

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2003-08-01
2020-04-02
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