@article{mbs:/content/journal/jmm/10.1099/jmm.0.05193-0, author = "Amar, Corinne F.L. and Dear, Paul H. and McLauchlin, Jim", title = "Detection and genotyping by real-time PCR/RFLP analyses of Giardia duodenalis from human faeces", journal= "Journal of Medical Microbiology", year = "2003", volume = "52", number = "8", pages = "681-683", doi = "https://doi.org/10.1099/jmm.0.05193-0", url = "https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.05193-0", publisher = "Microbiology Society", issn = "1473-5644", type = "Journal Article", abstract = "A nested PCR assay (TPILC-PCR) was developed to detect and distinguish between Giardia duodenalis assemblages A and B from human faeces by analysis of the triose phosphate isomerase gene (tpi). The assay comprised an initial multiplexed block-based amplification. This was followed by two separate real-time PCR assays specific for assemblages A and B using a LightCycler and SYBR Green I to identify PCR products by melting-point analysis. RFLP analysis was applied to distinguish G. duodenalis assemblage A groups I and II. The real-time nested PCR was evaluated using DNA extracted from purified giardial trophozoites, Cryptosporidium oocysts, whole faeces containing a range of potential pathogens (including G. duodenalis), faecal smears and bacterial suspensions. The assay was specific, sensitive, reproducible and rapid.", }