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Volume 60, Issue 9

Characterization of extended-spectrum β-lactamase-producing and isolate from the community in Morocco Free

Abstract

Of 803 community ( = 767) and ( = 36) isolates collected from patients with urinary tract infections in three Moroccan cities, 10 (1.3%) and 2 (5.6 %) isolates were shown to produce extended-spectrum β-lactamases (ESBLs). PFGE revealed that the isolates comprised seven distinct genotypes. The presence of plasmids in the 12 isolates was revealed by conjugation experiments of plasmids from these strains with KJ, with further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing indicated that the plasmids encoded the , , and genes, including genes for CTX-M-15 ( = 11), OXA-1 ( = 11), TEM-1b ( = 4), SHV-5 ( = 1) and SHV-1 ( = 2). Identification of plasmid-mediated quinolone-resistance genes was performed by PCR. The variant was detected in all strains, and two strains co-expressed , and genes. The presence of ESBLs in the strains studied was probably due to the dissemination of resistance plasmids with the predominant genotype of

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© 2011 SGM
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2011-09-01
2024-04-26
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Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco
J. Med. Microbiol. 60, 1344 (2011); https://doi.org/10.1099/jmm.0.032482-0
/content/journal/jmm/10.1099/jmm.0.032482-0
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