1887

Abstract

Of 803 community ( = 767) and ( = 36) isolates collected from patients with urinary tract infections in three Moroccan cities, 10 (1.3%) and 2 (5.6 %) isolates were shown to produce extended-spectrum β-lactamases (ESBLs). PFGE revealed that the isolates comprised seven distinct genotypes. The presence of plasmids in the 12 isolates was revealed by conjugation experiments of plasmids from these strains with KJ, with further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing indicated that the plasmids encoded the , , and genes, including genes for CTX-M-15 ( = 11), OXA-1 ( = 11), TEM-1b ( = 4), SHV-5 ( = 1) and SHV-1 ( = 2). Identification of plasmid-mediated quinolone-resistance genes was performed by PCR. The variant was detected in all strains, and two strains co-expressed , and genes. The presence of ESBLs in the strains studied was probably due to the dissemination of resistance plasmids with the predominant genotype of

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2011-09-01
2019-10-17
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References

  1. Andreu A. , Planells I. . Grupo Cooperativo Español para el Estudio de la Sensibilidad Antimicrobiana de los Patógenos Urinario ( 2008; ). [Etiology of community-acquired lower urinary infections and antimicrobial resistance of Escherichia coli: a national surveillance study]. Med Clin (Barc) 130:, 481–486 (in Spanish).[CrossRef]
    [Google Scholar]
  2. Arpin C. , Quentin C. , Grobost F. , Cambau E. , Robert J. , Dubois V. , Coulange L. , André C. . on behalf of the Scientific Committee of ONERBA ( 2009; ). Nationwide survey of extended-spectrum β-lactamase-producing Enterobacteriaceae in the French community setting. . J Antimicrob Chemother 63:, 1205–1214. [CrossRef].[PubMed]
    [Google Scholar]
  3. Barlow M. , Reik R. A. , Jacobs S. D. , Medina M. , Meyer M. P. , McGowan J. E. Jr , Tenover F. C. . ( 2008; ). High rate of mobilization for bla CTX-Ms. . Emerg Infect Dis 14:, 423–428.[CrossRef]
    [Google Scholar]
  4. Baudry P. J. , Nichol K. , DeCorby M. , Lagacé-Wiens P. , Olivier E. , Boyd D. , Mulvey M. R. , Hoban D. J. , Zhanel G. G. . ( 2009; ). Mechanisms of resistance and mobility among multidrug-resistant CTX-M-producing Escherichia coli from Canadian intensive care units: the 1st report of QepA in North America. . Diagn Microbiol Infect Dis 63:, 319–326. [CrossRef].[PubMed]
    [Google Scholar]
  5. Bourjilat F. , Dersi N. , Bouchrif B. , Amarouch H. , Timinouni M. . ( 2009; ). [Antibiotic resistance profile of community acquired uropathogenic E. coli in Morocco]. . Eur J Sci Res 38:, 57–62 (in French).
    [Google Scholar]
  6. Cantón R. , Novais A. , Valverde A. , Machado E. , Peixe L. , Baquero F. , Coque T. M. . ( 2008; ). Prevalence and spread of extended-spectrum β-lactamase-producing Enterobacteriaceae in Europe. . Clin Microbiol Infect 14: (Suppl. 1), 144–153. [CrossRef].[PubMed]
    [Google Scholar]
  7. Coque T. M. , Novais A. , Carattoli A. , Poirel L. , Pitout J. , Peixe L. , Baquero F. , Cantón R. , Nordmann P. . ( 2008; ). Dissemination of clonally related Escherichia coli strains expressing extended-spectrum β-lactamase CTX-M-15. . Emerg Infect Dis 14:, 195–200. [CrossRef].[PubMed]
    [Google Scholar]
  8. Crémet L. , Caroff N. , Dauvergne S. , Reynaud A. , Lepelletier D. , Corvec S. . ( 2011; ). Prevalence of plasmid-mediated quinolone resistance determinants in ESBL Enterobacteriaceae clinical isolates over a 1-year period in a French hospital. . Pathol Biol (Paris) 59:, 151–156.[CrossRef]
    [Google Scholar]
  9. Durmaz R. , Otlu B. , Koksal F. , Hosoglu S. , Ozturk R. , Ersoy Y. , Aktas E. , Gursoy N. C. , Caliskan A. . ( 2009; ). The optimization of a rapid pulsed-field gel electrophoresis protocol for the typing of Acinetobacter baumannii, Escherichia coli and Klebsiella spp. . Jpn J Infect Dis 62:, 372–377.[PubMed]
    [Google Scholar]
  10. Guessennd N. , Bremont S. , Gbonon V. , Kacou-Ndouba A. , Ekaza E. , Lambert T. , Dosso M. , Courvalin P. . ( 2008; ). [Qnr-type quinolone resistance in extended-spectrum β-lactamase producing enterobacteria in Abidjan, Ivory Coast]. . Pathol Biol (Paris) 56:, 439–446 (in French).[PubMed] [CrossRef]
    [Google Scholar]
  11. Hanson N. D. , Moland E. S. , Hong S. G. , Propst K. , Novak D. J. , Cavalieri S. J. . ( 2008; ). Surveillance of community-based reservoirs reveals the presence of CTX-M, imported AmpC, and OXA-30 β-lactamases in urine isolates of Klebsiella pneumoniae and Escherichia coli in a U.S. community. . Antimicrob Agents Chemother 52:, 3814–3816. [CrossRef].[PubMed]
    [Google Scholar]
  12. Harajly M. , Khairallah M. T. , Corkill J. E. , Araj G. F. , Matar G. M. . ( 2010; ). Frequency of conjugative transfer of plasmid-encoded ISEcp1bla CTX-M-15 and aac(6′)-lb-cr genes in Enterobacteriaceae at a tertiary care center in Lebanon – role of transferases. . Ann Clin Microbiol Antimicrob 9:, 19. [CrossRef].[PubMed]
    [Google Scholar]
  13. Iabadene H. , Messai Y. , Ammari H. , Ramdani-Bouguessa N. , Lounes S. , Bakour R. , Arlet G. . ( 2008; ). Dissemination of ESBL and Qnr determinants in Enterobacter cloacae in Algeria. . J Antimicrob Chemother 62:, 133–136. [CrossRef].[PubMed]
    [Google Scholar]
  14. Jiang Y. , Zhou Z. , Qian Y. , Wei Z. , Yu Y. , Hu S. , Li L. . ( 2008; ). Plasmid-mediated quinolone resistance determinants qnr and aac(6′)-Ib-cr in extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae in China. . J Antimicrob Chemother 61:, 1003–1006. [CrossRef].[PubMed]
    [Google Scholar]
  15. Jones G. L. , Warren R. E. , Skidmore S. J. , Davies V. A. , Gibreel T. , Upton M. . ( 2008; ). Prevalence and distribution of plasmid-mediated quinolone resistance genes in clinical isolates of Escherichia coli lacking extended-spectrum β-lactamases. . J Antimicrob Chemother 62:, 1245–1251. [CrossRef].[PubMed]
    [Google Scholar]
  16. Kariuki S. , Revathi G. , Corkill J. , Kiiru J. , Mwituria J. , Mirza N. , Hart C. A. . ( 2007; ). Escherichia coli from community-acquired urinary tract infections resistant to fluoroquinolones and extended-spectrum β-lactams. . J Infect Dev Ctries 1:, 257–262.[PubMed]
    [Google Scholar]
  17. Kiratisin P. , Apisarnthanarak A. , Laesripa C. , Saifon P. . ( 2008; ). Molecular characterization and epidemiology of extended-spectrum-β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates causing health care-associated infection in Thailand, where the CTX-M family is endemic. . Antimicrob Agents Chemother 52:, 2818–2824.[CrossRef]
    [Google Scholar]
  18. Lahlou Amine I. , Chegri M. , L'Kassmi H. . ( 2009; ). [Epidemiology and antibiotic resistance of Enterobacteriaceae isolated in urinary tract infections at the Moulay-Ismail Military Hospital of Meknes]. . Antibiotiques 11:, 90–96 (in French).[CrossRef]
    [Google Scholar]
  19. Lavollay M. , Mamlouk K. , Frank T. , Akpabie A. , Burghoffer B. , Ben Redjeb S. , Bercion R. , Gautier V. , Arlet G. . ( 2006; ). Clonal dissemination of a CTX-M-15 β-lactamase-producing Escherichia coli strain in the Paris area, Tunis, and Bangui. . Antimicrob Agents Chemother 50:, 2433–2438. [CrossRef].[PubMed]
    [Google Scholar]
  20. Livermore D. M. , Hawkey P. M. . ( 2005; ). CTX-M: changing the face of ESBLs in the UK. . J Antimicrob Chemother 56:, 451–454. [CrossRef].[PubMed]
    [Google Scholar]
  21. Ma J. , Zeng Z. , Chen Z. , Xu X. , Wang X. , Deng Y. , D. , Huang L. , Zhang Y. et al. ( 2009; ). High prevalence of plasmid-mediated quinolone resistance determinants qnr, aac(6′)-Ib-cr, and qepA among ceftiofur-resistant Enterobacteriaceae isolates from companion and food-producing animals. . Antimicrob Agents Chemother 53:, 519–524. [CrossRef].[PubMed]
    [Google Scholar]
  22. Macrina F. L. , Kopecko D. J. , Jones K. R. , Ayers D. J. , McCowen S. M. . ( 1978; ). A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. . Plasmid 1:, 417–420. [CrossRef].[PubMed]
    [Google Scholar]
  23. Mendonça N. , Leitão J. , Manageiro V. , Ferreira E. , Caniça M. . ( 2007; ). Spread of extended-spectrum β-lactamase CTX-M-producing Escherichia coli clinical isolates in community and nosocomial environments in Portugal. . Antimicrob Agents Chemother 51:, 1946–1955. [CrossRef].[PubMed]
    [Google Scholar]
  24. Minarini L. A. , Camargo I. L. , Pitondo-Silva A. , Darini A. L. . ( 2007a; ). Multilocus sequence typing of uropathogenic ESBL-producing Escherichia coli isolated in a Brazilian community. . Curr Microbiol 55:, 524–529. [CrossRef].[PubMed]
    [Google Scholar]
  25. Minarini L. A. , Gales A. C. , Palazzo I. C. , Darini A. L. . ( 2007b; ). Prevalence of community-occurring extended spectrum β-lactamase-producing Enterobacteriaceae in Brazil. . Curr Microbiol 54:, 335–341. [CrossRef].[PubMed]
    [Google Scholar]
  26. Mohamudha P. R. , Harish B. N. , Parija S. C. . ( 2010; ). AmpC β lactamases among Gram negative clinical isolates from a tertiary hospital, South India. . Braz J Microbiol 41:, 596–602. [CrossRef]
    [Google Scholar]
  27. Mshana S. E. , Imirzalioglu C. , Hossain H. , Hain T. , Domann E. , Chakraborty T. . ( 2009; ). Conjugative IncFI plasmids carrying CTX-M-15 among Escherichia coli ESBL producing isolates at a university hospital in Germany. . BMC Infect Dis 9:, 97. [CrossRef].[PubMed]
    [Google Scholar]
  28. Nordmann P. , Poirel L. . ( 2005; ). Emergence of plasmid-mediated resistance to quinolones in Enterobacteriaceae . . J Antimicrob Chemother 56:, 463–469. [CrossRef].[PubMed]
    [Google Scholar]
  29. Périchon B. , Courvalin P. , Galimand M. . ( 2007; ). Transferable resistance to aminoglycosides by methylation of G1405 in 16S rRNA and to hydrophilic fluoroquinolones by QepA-mediated efflux in Escherichia coli . . Antimicrob Agents Chemother 51:, 2464–2469. [CrossRef].[PubMed]
    [Google Scholar]
  30. Pitout J. D. , Hanson N. D. , Church D. L. , Laupland K. B. . ( 2004; ). Population-based laboratory surveillance for Escherichia coli-producing extended-spectrum β-lactamases: importance of community isolates with bla CTX-M genes. . Clin Infect Dis 38:, 1736–1741. [CrossRef].[PubMed]
    [Google Scholar]
  31. Pitout J. D. , Gregson D. B. , Church D. L. , Elsayed S. , Laupland K. B. . ( 2005a; ). Community-wide outbreaks of clonally related CTX-M-14 β-lactamase-producing Escherichia coli strains in the Calgary health region. . J Clin Microbiol 43:, 2844–2849. [CrossRef].[PubMed]
    [Google Scholar]
  32. Pitout J. D. , Nordmann P. , Laupland K. B. , Poirel L. . ( 2005b; ). Emergence of Enterobacteriaceae producing extended-spectrum β-lactamases (ESBLs) in the community. . J Antimicrob Chemother 56:, 52–59.[CrossRef]
    [Google Scholar]
  33. Pitout J. D. , Campbell L. , Church D. L. , Gregson D. B. , Laupland K. B. . ( 2009; ). Molecular characteristics of travel-related extended-spectrum-β-lactamase-producing Escherichia coli isolates from the Calgary health region. . Antimicrob Agents Chemother 53:, 2539–2543. [CrossRef].[PubMed]
    [Google Scholar]
  34. Reinert R. R. , Low D. E. , Rossi F. , Zhang X. , Wattal C. , Dowzicky M. J. . ( 2007; ). Antimicrobial susceptibility among organisms from the Asia/Pacific Rim, Europe and Latin and North America collected as part of TEST and the in vitro activity of tigecycline. . J Antimicrob Chemother 60:, 1018–1029. [CrossRef].[PubMed]
    [Google Scholar]
  35. Robicsek A. , Jacoby G. A. , Hooper D. C. . ( 2006; ). The worldwide emergence of plasmid-mediated quinolone resistance. . Lancet Infect Dis 6:, 629–640. [CrossRef].[PubMed]
    [Google Scholar]
  36. Rodríguez-Baño J. , Navarro M. D. , Romero L. , Martínez-Martínez L. , Muniain M. A. , Perea E. J. , Pérez-Cano R. , Pascual A. . ( 2004; ). Epidemiology and clinical features of infections caused by extended-spectrum β-lactamase-producing Escherichia coli in nonhospitalized patients. . J Clin Microbiol 42:, 1089–1094. [CrossRef].[PubMed]
    [Google Scholar]
  37. Rodríguez-Baño J. , Alcalá J. , Cisneros J. M. , Grill F. , Oliver A. , Horcajada J. P. , Tórtola T. , Mirelis B. , Navarro G. et al. ( 2009; ). Escherichia coli producing SHV-type extended-spectrum β-lactamase is a significant cause of community-acquired infection. . J Antimicrob Chemother 63:, 781–784. [CrossRef].[PubMed]
    [Google Scholar]
  38. Romero L. , López L. , Rodríguez-Baño J. , Ramón Hernández J. , Martínez-Martínez L. , Pascual A. . ( 2005; ). Long-term study of the frequency of Escherichia coli and Klebsiella pneumoniae isolates producing extended-spectrum β-lactamases. . Clin Microbiol Infect 11:, 625–631.[CrossRef]
    [Google Scholar]
  39. Ruppé E. . ( 2010; ). [Epidemiology of expanded-spectrum β-lactamases: the rise of CTX-M]. . Antibiotiques 12:, 3–16 (in French).[CrossRef]
    [Google Scholar]
  40. Ruppé E. , Woerther P. L. , Diop A. , Sene A. M. , Da Costa A. , Arlet G. , Andremont A. , Rouveix B. . ( 2009; ). Carriage of CTX-M-15-producing Escherichia coli isolates among children living in a remote village in Senegal. . Antimicrob Agents Chemother 53:, 3135–3137. [CrossRef].[PubMed]
    [Google Scholar]
  41. Sekhsokh Y. , Chadli M. , El Hamzaoui S. A. . ( 2008; ). [Frequency and antibiotic susceptibility of bacteria identified in urine]. . Med Mal Infect 38:, 324–327 (in French). [CrossRef].[PubMed]
    [Google Scholar]
  42. Shi W. F. , Zhou J. , Qin J. P. . ( 2009; ). Transconjugation and genotyping of the plasmid-mediated AmpC β-lactamase and extended-spectrum β-lactamase genes in Klebsiella pneumoniae . . Chin Med J (Engl) 122:, 1092–1096.[PubMed]
    [Google Scholar]
  43. Soge O. O. , Adeniyi B. A. , Roberts M. C. . ( 2006; ). New antibiotic resistance genes associated with CTX-M plasmids from uropathogenic Nigerian Klebsiella pneumoniae . . J Antimicrob Chemother 58:, 1048–1053. [CrossRef].[PubMed]
    [Google Scholar]
  44. Walsh T. R. , Toleman M. A. , Poirel L. , Nordmann P. . ( 2005; ). Metallo-β-lactamases: the quiet before the storm?. Clin Microbiol Rev 18:, 306–325. [CrossRef].[PubMed]
    [Google Scholar]
  45. Walther-Rasmussen J. , Høiby N. . ( 2006; ). OXA-type carbapenemases. . J Antimicrob Chemother 57:, 373–383. [CrossRef].[PubMed]
    [Google Scholar]
  46. Woodford N. , Kaufmann M. E. , Karisik E. , Hartley J. W. . ( 2007; ). Molecular epidemiology of multiresistant Escherichia coli isolates from community-onset urinary tract infections in Cornwall, England. . J Antimicrob Chemother 59:, 106–109. [CrossRef].[PubMed]
    [Google Scholar]
  47. Younes A. , Hamouda A. , Dave J. , Amyes S. G. . ( 2011; ). Prevalence of transferable bla CTX-M-15 from hospital- and community-acquired Klebsiella pneumoniae isolates in Scotland. . J Antimicrob Chemother 66:, 313–318. [CrossRef].[PubMed]
    [Google Scholar]
  48. Yumuk Z. , Afacan G. , Nicolas-Chanoine M. H. , Sotto A. , Lavigne J. P. . ( 2008; ). Turkey: a further country concerned by community-acquired Escherichia coli clone O25-ST131 producing CTX-M-15. . J Antimicrob Chemother 62:, 284–288.[CrossRef]
    [Google Scholar]
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