RT Journal Article SR Electronic(1) A1 Barguigua, Abouddihaj A1 El Otmani, Fatima A1 Talmi, Mustapha A1 Bourjilat, Fatna A1 Haouzane, Fatima A1 Zerouali, Khalid A1 Timinouni, MohammedYR 2011 T1 Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco JF Journal of Medical Microbiology, VO 60 IS 9 SP 1344 OP 1352 DO https://doi.org/10.1099/jmm.0.032482-0 PB Microbiology Society, SN 1473-5644, AB Of 803 community Escherichia coli (n = 767) and Klebsiella pneumoniae (n = 36) isolates collected from patients with urinary tract infections in three Moroccan cities, 10 E. coli (1.3%) and 2 K. pneumoniae (5.6 %) isolates were shown to produce extended-spectrum β-lactamases (ESBLs). PFGE revealed that the E. coli isolates comprised seven distinct genotypes. The presence of plasmids in the 12 isolates was revealed by conjugation experiments of plasmids from these Enterobacteriaceae strains with E. coli K12J5, with further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing indicated that the plasmids encoded the bla CTX-M, bla OXA, bla TEM and bla SHV genes, including genes for CTX-M-15 (n = 11), OXA-1 (n = 11), TEM-1b (n = 4), SHV-5 (n = 1) and SHV-1 (n = 2). Identification of plasmid-mediated quinolone-resistance genes was performed by PCR. The aac(6′)Ib-cr variant was detected in all strains, and two strains co-expressed qnrS1, bla CTX-M-15 and bla OXA-1 genes. The presence of ESBLs in the Enterobacteriaceae strains studied was probably due to the dissemination of resistance plasmids with the predominant genotype of bla CTX-M-15. , UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.032482-0