1887

Abstract

This study examined in detail the population structure of from healthy adults with respect to the prevalence of antibiotic resistance and specific resistance determinants. isolated from the faeces of 20 healthy adults not recently exposed to antibiotics was tested for resistance to ten antibiotics and for carriage of integrons and resistance determinants using PCR. Strain diversity was assessed using biochemical and molecular criteria. was present in 19 subjects at levels ranging from 2.0×10 to 1.7×10 c.f.u. (g faeces). Strains resistant to one to six antibiotics were found at high levels (>30 %) in only ten individuals, but at significant levels (>0.5 %) in 14. Resistant isolates with the same phenotype from the same individual were indistinguishable, but more than one susceptible strain was sometimes found. Overall, individuals harboured one to four strains, although in 17 samples one strain was dominant (>70 % of isolates). Eighteen strains resistant to ampicillin, sulfamethoxazole, tetracycline and trimethoprim in 15 different combinations were observed. One resistant strain was carried by two unrelated individuals and a susceptible strain was shared by two cohabiting subjects. Two minority strains were derivatives of a more abundant resistant strain in the same sample, showing that continuous evolution is occurring . The trimethoprim-resistance genes , , , or were in cassettes in a class 1 or class 2 integron. Ampicillin resistance was conferred by the gene, sulfamethoxazole resistance by , or and tetracycline resistance by (A) or (B). Chloramphenicol resistance ( gene) was detected only once. Phylogenetic groups A and B2 were more common than B1 and D. Commensal of healthy humans represent an important reservoir for numerous antibiotic-resistance genes in many combinations. However, measuring the true extent of resistance carriage in commensal requires in-depth analysis.

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2010-11-01
2019-10-22
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