1887

Abstract

The gene complex in encodes an efflux pump that is responsible for export of antibacterial hydrophobic agents. The promoter region of the operon harbours an insertion sequence known as a Correia element, and a binding site for the integration host factor (IHF) is present at the centre of the Correia element. It has been suggested that the expression of the operon in meningococci is subject to transcriptional regulation by the IHF and post-transcriptional regulation by cleavage in the inverted repeat of the Correia element. The promoter region of the operon as well as the association of changes at that point with decreased susceptibility to antimicrobial drugs in 606 strains were analysed in this study. Two different deletions were present in the analysed region. The first one, found in seven strains, corresponded to absence of the Correia element. The second one, affecting the −10 region and first 100 bp of the gene and present in 57 isolates, was only found in ST-1624 isolates. None of the deletions were associated with decreased susceptibility to antimicrobial drugs. Although most of the meningococcal strains carry the Correia element at that position, its deletion is not an exception.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.021220-0
2010-09-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/59/9/1055.html?itemId=/content/journal/jmm/10.1099/jmm.0.021220-0&mimeType=html&fmt=ahah

References

  1. Abadi, F. J., Carter, P. E., Cash, P. & Pennington, T. H. ( 1996; ). Rifampin resistance in Neisseria meningitidis due to alterations in membrane permeability. Antimicrob Agents Chemother 40, 646–651.
    [Google Scholar]
  2. Buisine, N., Tang, C. M. & Chalmers, R. ( 2002; ). Transposon-like Correia elements: structure, distribution and genetic exchange between pathogenic Neisseria sp. FEBS Lett 522, 52–58.[CrossRef]
    [Google Scholar]
  3. CLSI ( 2007; ). Performance Standards for Antimicrobial Susceptibility Testing; 15th Informational Supplement, M100-S15. Wayne, PA: Clinical and Laboratory Standards Institute.
  4. Corso, A., Faccone, D., Miranda, M., Rodriguez, M., Regueira, M., Carranza, C., Vencina, C., Vazquez, J. A. & Galas, M. ( 2005; ). Emergence of Neisseria meningitidis with decreased susceptibility to ciprofloxacin in Argentina. J Antimicrob Chemother 55, 596–597.[CrossRef]
    [Google Scholar]
  5. Delahay, R. M., Robertson, B. D., Balthazar, J. T., Shafer, W. M. & Ison, C. A. ( 1997; ). Involvement of the gonococcal MtrE protein in the resistance of Neisseria gonorrhoeae to toxic hydrophobic agents. Microbiology 143, 2127–2133.[CrossRef]
    [Google Scholar]
  6. Dewi, B. E., Akira, S., Hayashi, H. & Ba-Thein, W. ( 2004; ). High occurrence of simultaneous mutations in target enzyme and MtrRCDE efflux system in quinolone-resistant Neisseria gonorrhoeae. Sex Transm Dis 31, 353–359.[CrossRef]
    [Google Scholar]
  7. Enríquez, R., Abad, R., Salcedo, C., Pérez, S. & Vázquez, J. A. ( 2008; ). Fluoroquinolone resistance in Neisseria meningitidis in Spain. J Antimicrob Chemother 61, 286–290.
    [Google Scholar]
  8. Maiden, M. C., Bygraves, J. A., Feil, E., Morelli, G., Russell, J. E., Urwin, R., Zhang, Q., Zhou, J., Zurth, K. & other authors ( 1998; ). Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A 95, 3140–3145.[CrossRef]
    [Google Scholar]
  9. Parkhill, J., Achtman, M., James, K. D., Bentley, S. D., Churcher, C., Klee, S. R., Morelli, G., Basham, D., Brown, D. & other authors ( 2000; ). Complete DNA sequence of a serogroup A strain of Neisseria meningitidis Z2491. Nature 404, 502–506.[CrossRef]
    [Google Scholar]
  10. Rouquette-Loughlin, C. E., Balthazar, J. T., Hill, S. A. & Shafer, W. M. ( 2004; ). Modulation of the mtrCDE-encoded efflux pump gene complex of Neisseria meningitidis due to a Correia element insertion sequence. Mol Microbiol 54, 731–741.[CrossRef]
    [Google Scholar]
  11. Shultz, T. R., White, P. A. & Tapsall, J. W. ( 2005; ). In vitro assessment of the further potential for development of fluoroquinolone resistance in Neisseria meningitidis. Antimicrob Agents Chemother 49, 1753–1760.[CrossRef]
    [Google Scholar]
  12. Stefanelli, P., Fazio, C., La Rosa, G., Marianelli, C., Muscillo, M. & Mastrantonio, P. ( 2001; ). Rifampicin-resistant meningococci causing invasive disease: detection of point mutations in the rpoB gene and molecular characterization of the strains. J Antimicrob Chemother 47, 219–222.[CrossRef]
    [Google Scholar]
  13. Tettelin, H., Saunders, N. J., Heidelberg, J., Jeffries, A. C., Nelson, K. E., Eisen, J. A., Ketchum, K. A., Hood, D. W., Peden, J. F. & other authors ( 2000; ). Complete genome sequence of Neisseria meningitidis serogroup B strain MC58. Science 287, 1809–1815.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.021220-0
Loading
/content/journal/jmm/10.1099/jmm.0.021220-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error