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Volume 72, Issue 6

Immunoassay-based evaluation of rOmp28 protein as a candidate for the identification of species No Access

Abstract

Brucellosis is an important bacterial zoonosis, re-emerging as a serious public health concern in developing countries. Two major species, and , cause recurrent facile infection in human. Therefore, rapid and accurate diagnosis for early disease control and prevention is needed in areas with low disease burden.

This study evaluated the sandwich enzyme-linked immunosorbent assay (ELISA) (S-ELISA) immunoassay for potential use of whole-cell (WC) and recombinant outer-membrane protein (rOmp28)-derived IgG polyclonals in sensitive detection of .

Immunoassay-based WC detection of species in important sub-clinical matrices at lower limits of detection.

We purified recombinant rOmp28 with Ni–NTA gel affinity chromatography and produced IgG polyclonal antibodies (pAbs) using BALB/c mice and New Zealand white female rabbits against different antigens (Ags) of . Checkerboard sandwich ELISA and P/N ratio (optical density of ‘P’ positive test sample to ‘N’ negative control) were used for evaluation and optimization of the study. The pAbs were characterized using Western blot analysis and different matrices were spiked with WC Ag of .

Double-antibody S-ELISA was developed using WC Ag-derived rabbit IgG (capture antibody at 10 µg ml) and rOmp28-derived mice IgG (detection antibody at 100 µg ml) with a detection range of 10 to 10 cells ml and a limit of detection at 10 cells ml. A P/N ratio of 1.1 was obtained with WC pAbs as compared to 0.6 and 0.9 ratios with rOmp28-derived pAbs for detecting 16M and S99, respectively. An increased P/N ratio of 4.4 was obtained with WC Ag-derived rabbit IgG as compared to 4.2>4.1>2.4 ratios obtained with rabbit IgGs derived against cell envelope (CE), rOmp28 and sonicated antigen (SA) of with high affinity for rOmp28 Ag analysed on immunoblots. The rOmp28-derived mice IgG revealed two species at P/N ratios of 11.8 and 6.3, respectively. Upon validation, S-ELISA detected WCs in human whole blood and sera samples with no cross-reactivity to other related bacteria.

The developed S-ELISA is specific and sensitive in early detection of from different matrices of clinical and non-clinical disease presentation.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Brucella , polyclonal antibody , rOmp28 , sandwich ELISA and whole-cell detection
© 2023 The Authors
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2023-06-27
2024-05-02
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References

  1. Lindahl-Rajala E, Hoffman T, Fretin D, Godfroid J, Sattorov N et al. Detection and characterization of Brucella spp. in bovine milk in small-scale urban and peri-urban farming in Tajikistan. PLoS Negl Trop Dis 2017; 11:e0005367 [View Article] [PubMed]
     [Google Scholar]
  2. Franc KA, Krecek RC, Häsler BN, Arenas-Gamboa AM. Brucellosis remains a neglected disease in the developing world: a call for interdisciplinary action. BMC Public Health 2018; 18:125 [View Article] [PubMed]
     [Google Scholar]
  3. Avila-Calderón ED, Lopez-Merino A, Sriranganathan N, Boyle SM, Contreras-Rodríguez A. A history of the development of Brucella vaccines. Biomed Res Int 2013; 2013:743509 [View Article] [PubMed]
     [Google Scholar]
  4. Dadar M, Tiwari R, Sharun K, Dhama K. Importance of brucellosis control programs of livestock on the improvement of one health. Vet Q 2021; 41:137–151 [View Article] [PubMed]
     [Google Scholar]
  5. Bakri FG, AlQadiri HM, Adwan MH. The highest cited papers in Brucellosis: identification using two databases and review of the papers’ major findings. Biomed Res Int 2018; 2018:9291326 [View Article] [PubMed]
     [Google Scholar]
  6. Bruce D. Note on the recovery of a microorganism in Malta fever. Practitioner 1887; 39:161
     [Google Scholar]
  7. Bang B. The etiology of epizootic abortion. J Comp Pathol Ther 1897; 10:125–IN2 [View Article]
     [Google Scholar]
  8. Wyatt HV. How Themistocles Zammit found Malta fever (brucellosis) to be transmitted by the milk of goats. J R Soc Med 2005; 98:451–454 [View Article] [PubMed]
     [Google Scholar]
  9. Wyatt HV. Lessons from the history of brucellosis. Rev Sci Tech 2013; 32:17–25 [View Article] [PubMed]
     [Google Scholar]
  10. Van der Henst C, de Barsy M, Zorreguieta A, Letesson J-J, De Bolle X. The Brucella pathogens are polarized bacteria. Microbes Infect 2013; 15:998–1004 [View Article] [PubMed]
     [Google Scholar]
  11. Christopher S, Umapathy BL, Ravikumar KL. Brucellosis: review on the recent trends in pathogenicity and laboratory diagnosis. J Lab Physicians 2010; 2:55–60 [View Article] [PubMed]
     [Google Scholar]
  12. Olsen SC, Boggiatto P, White DM, McNunn T. Biosafety concerns related to Brucella and its potential use as a bioweapon. Appl Biosaf 2018; 23:77–90 [View Article]
     [Google Scholar]
  13. Hisham Y, Ashhab Y. Identification of cross-protective potential antigens against pathogenic Brucella spp. through combining pan-genome analysis with reverse vaccinology. J Immunol Res 2018; 2018:1474517 [View Article] [PubMed]
     [Google Scholar]
  14. Hull NC, Schumaker BA. Comparisons of brucellosis between human and veterinary medicine. Infect Ecol Epidemiol 2018; 8:1500846 [View Article] [PubMed]
     [Google Scholar]
  15. Liu F, Li J-M, Zeng F-L, Zong Y, Leng X et al. Prevalence and risk factors of Brucellosis, Chlamydiosis, and Bluetongue among Sika deer in Jilin province in China. Vector Borne Zoonotic Dis 2018; 18:226–230 [View Article] [PubMed]
     [Google Scholar]
  16. Buzgan T, Karahocagil MK, Irmak H, Baran AI, Karsen H et al. Clinical manifestations and complications in 1028 cases of brucellosis: a retrospective evaluation and review of the literature. Int J Infect Dis 2010; 14:e469–78 [View Article] [PubMed]
     [Google Scholar]
  17. Godfroid J, Al Dahouk S, Pappas G, Roth F, Matope G et al. A “One Health” surveillance and control of brucellosis in developing countries: moving away from improvisation. Comp Immunol Microbiol Infect Dis 2013; 36:241–248 [View Article] [PubMed]
     [Google Scholar]
  18. Köse Ş, Serin Senger S, Akkoçlu G, Kuzucu L, Ulu Y et al. Clinical manifestations, complications, and treatment of brucellosis: evaluation of 72 cases. Turk J Med Sci 2014; 44:220–223 [View Article] [PubMed]
     [Google Scholar]
  19. Di Bonaventura G, Angeletti S, Ianni A, Petitti T, Gherardi G. Microbiological laboratory diagnosis of human Brucellosis: an overview. Pathogens 2021; 10:1623 [View Article] [PubMed]
     [Google Scholar]
  20. Franco MP, Mulder M, Gilman RH, Smits HL. Human brucellosis. Lancet Infect Dis 2007; 7:775–786 [View Article] [PubMed]
     [Google Scholar]
  21. Zhang N, Zhou H, Huang D-S, Guan P, Samy AM. Brucellosis awareness and knowledge in communities worldwide: a systematic review and meta-analysis of 79 observational studies. PLoS Negl Trop Dis 2019; 13:e0007366 [View Article] [PubMed]
     [Google Scholar]
  22. Zhou K, Wu B, Pan H, Paudyal N, Jiang J et al. ONE health approach to address Zoonotic Brucellosis: a spatiotemporal associations study between animals and Hhmans. Front Vet Sci 2020; 7:521 [View Article] [PubMed]
     [Google Scholar]
  23. Amro A, Mansoor B, Hamarsheh O, Hjaija D. Recent trends in human brucellosis in the West Bank, Palestine. Int J Infect Dis 2021; 106:308–313 [View Article] [PubMed]
     [Google Scholar]
  24. Celebi G, Külah C, Kiliç S, Ustündağ G. Asymptomatic Brucella bacteraemia and isolation of Brucella melitensis biovar 3 from human breast milk. Scand J Infect Dis 2007; 39:205–208 [View Article] [PubMed]
     [Google Scholar]
  25. Smits HL. Brucellosis in pastoral and confined livestock: prevention and vaccination. Rev Sci Tech 2013; 32:219–228 [View Article] [PubMed]
     [Google Scholar]
  26. Khan MZ, Zahoor M. An overview of brucellosis in cattle and humans, and its serological and molecular diagnosis in control strategies. Trop Med Infect Dis 2018; 3:65 [View Article] [PubMed]
     [Google Scholar]
  27. Zheng R, Xie S, Lu X, Sun L, Zhou Y et al. A systematic review and meta-analysis of epidemiology and clinical manifestations of human Brucellosis in China. Biomed Res Int 2018; 2018:5712920 [View Article] [PubMed]
     [Google Scholar]
  28. Bosilkovski M, Stojovski M, Siskova D, Ridov A, Kostoska E et al. Brucellosis in pregnancy: case reports with different outcomes in an endemic region. Acta Clin Croat 2020; 59:338–343 [View Article] [PubMed]
     [Google Scholar]
  29. Ben Lahlou Y, Benaissa E, Maleb A, Chadli M, Elouennass M. Pancytopenia revealing acute brucellosis. IDCases 2021; 23:e01037 [View Article]
     [Google Scholar]
  30. Rossetti CA, Arenas-Gamboa AM, Maurizio E. Caprine brucellosis: a historically neglected disease with significant impact on public health. PLoS Negl Trop Dis 2017; 11:e0005692 [View Article] [PubMed]
     [Google Scholar]
  31. González-Espinoza G, Arce-Gorvel V, Mémet S, Gorvel J-P. Brucella: reservoirs and niches in animals and humans. Pathogens 2021; 10:186 [View Article] [PubMed]
     [Google Scholar]
  32. O’Callaghan D. Human brucellosis: recent advances and future challenges. Infect Dis Poverty 2020; 9:101 [View Article] [PubMed]
     [Google Scholar]
  33. Khurana SK, Sehrawat A, Tiwari R, Prasad M, Gulati B et al. Bovine brucellosis - a comprehensive review. Vet Q 2021; 41:61–88 [View Article] [PubMed]
     [Google Scholar]
  34. Yagupsky P. Preventing laboratory-acquired Brucellosis in the era of MALDI-TOF technology and molecular tests: a narrative review. Zoonotic Diseases 2022; 2:172–182 [View Article]
     [Google Scholar]
  35. Godfroid J, Cloeckaert A, Liautard J-P, Kohler S, Fretin D et al. From the discovery of the Malta fever’s agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Vet Res 2005; 36:313–326 [View Article] [PubMed]
     [Google Scholar]
  36. Lindahl JF, Vrentas CE, Deka RP, Hazarika RA, Rahman H et al. Brucellosis in India: results of a collaborative workshop to define one health priorities. Trop Anim Health Prod 2020; 52:387–396 [View Article] [PubMed]
     [Google Scholar]
  37. Clavijo E, Díaz R, Anguita A, García A, Pinedo A et al. Comparison of a dipstick assay for detection of Brucella-specific immunoglobulin M antibodies with other tests for serodiagnosis of human brucellosis. Clin Diagn Lab Immunol 2003; 10:612–615 [View Article] [PubMed]
     [Google Scholar]
  38. Yohannes M, Gill JPS, Ghatak S, Singh DK, Tolosa T. Comparative evaluation of the rose bengal plate test, standard tube agglutination test and complement fixation test for the diagnosis of human brucellosis. Rev Sci Tech 2012; 31:979–984 [View Article] [PubMed]
     [Google Scholar]
  39. Tiwari S, Kumar A, Thavaselvam D, Mangalgi S, Rathod V et al. Development and comparative evaluation of a plate enzyme-linked immunosorbent assay based on recombinant outer membrane antigens Omp28 and Omp31 for diagnosis of human brucellosis. Clin Vaccine Immunol 2013; 20:1217–1222 [View Article] [PubMed]
     [Google Scholar]
  40. Peeridogaheh H, Golmohammadi MG, Pourfarzi F. Evaluation of ELISA and brucellacapt tests for diagnosis of human brucellosis. Iran J Microbiol 2013; 5:14–18 [PubMed]
     [Google Scholar]
  41. Baruch J, Suanes A, Piaggio JM, Gil AD. Analytic sensitivity of an ELISA test on pooled sera samples for detection of bovine brucellosis in eradication stages in Uruguay. Front Vet Sci 2020; 7:178 [View Article] [PubMed]
     [Google Scholar]
  42. Ozdemir M, Feyzioglu B, Kurtoglu MG, Dogan M, Dagi HT et al. A comparison of Immunocapture agglutination and ELISA methods in serological diagnosis of brucellosis. Int J Med 2011; 8:428–432
     [Google Scholar]
  43. Patra KP, Saito M, Atluri VL, Rolán HG, Young B et al. A protein-conjugate approach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis. PLoS Negl Trop Dis 2014; 8:e2926 [View Article] [PubMed]
     [Google Scholar]
  44. Gurbilek SE, Tel OY, Keskin O. Comparative evaluation of three serological tests for the detection of Brucella antibodies from infected cattle herds. J Appl Anim Res 2016; 45:557–559
     [Google Scholar]
  45. Golchin M, Mollayi S, Mohammadi E, Eskandarzade N. Development of a diagnostic indirect ELISA test for detection of Brucella antibody using recombinant outer membrane protein 16 kDa (rOMP16). Vet Res Forum 2022; 13:387–391 [View Article] [PubMed]
     [Google Scholar]
  46. Bhartiya NM, Husain AA, Daginawala HF, Singh L, Kashyap RS. Development of an immunodiagnostic test for screening human brucellosis cases using the whole-cell antigens of Brucella abortus. Malays J Med Sci 2020; 27:15–26 [View Article] [PubMed]
     [Google Scholar]
  47. Xu N, Wang W, Chen F, Li W, Wang G. ELISA is superior to bacterial culture and agglutination test in the diagnosis of brucellosis in an endemic area in China. BMC Infect Dis 2020; 20:11 [View Article] [PubMed]
     [Google Scholar]
  48. Ahmed IM, Khairani-Bejo S, Hassan L, Bahaman AR, Omar AR. Serological diagnostic potential of recombinant outer membrane proteins (rOMPs) from Brucella melitensis in mouse model using indirect enzyme-linked immunosorbent assay. BMC Vet Res 2015; 11:275 [View Article] [PubMed]
     [Google Scholar]
  49. Bulashev A, Jakubowski T, Tursunov K, Kiyan V, Zhumalin A. Immunogenicity and antigenicity of Brucella recombinant outer membrane proteins. Vet Med Zoot 2018; 76:98
     [Google Scholar]
  50. Lindler LE, Hadfield TL, Tall BD, Snellings NJ, Rubin FA et al. Cloning of a Brucella melitensis group 3 antigen gene encoding Omp28, a protein recognized by the humoral immune response during human brucellosis. Infect Immun 1996; 64:2490–2499 [View Article] [PubMed]
     [Google Scholar]
  51. Thavaselvam D, Kumar A, Tiwari S, Mishra M, Prakash A. Cloning and expression of the immunoreactive Brucella melitensis 28 kDa outer-membrane protein (Omp28) encoding gene and evaluation of the potential of Omp28 for clinical diagnosis of brucellosis. J Med Microbiol 2010; 59:421–428 [View Article] [PubMed]
     [Google Scholar]
  52. Seco-Mediavilla P, Verger J-M, Grayon M, Cloeckaert A, Marín CM et al. Epitope mapping of the Brucella melitensis BP26 immunogenic protein: usefulness for diagnosis of sheep brucellosis. Clin Diagn Lab Immunol 2003; 10:647–651 [View Article] [PubMed]
     [Google Scholar]
  53. Manat Y, Shustov AV, Evtehova E, Eskendirova SZ. Expression, purification and immunochemical characterization of recombinant OMP28 protein of Brucella species. Open Vet J 2016; 6:71–77 [View Article] [PubMed]
     [Google Scholar]
  54. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193:265–275 [PubMed]
     [Google Scholar]
  55. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227:680–685 [View Article] [PubMed]
     [Google Scholar]
  56. Arenas-Gamboa AM, Ficht TA, Kahl-McDonagh MM, Rice-Ficht AC. Immunization with a single dose of a microencapsulated Brucella melitensis mutant enhances protection against wild-type challenge. Infect Immun 2008; 76:2448–2455 [View Article] [PubMed]
     [Google Scholar]
  57. Adone R, Muscillo M, La Rosa G, Francia M, Tarantino M. Antigenic, immunologic and genetic characterization of rough strains B. abortus RB51, B. melitensis B115 and B. melitensis B18. PLoS One 2011; 6:e24073 [View Article] [PubMed]
     [Google Scholar]
  58. Yang Y, Yin J, Guo D, Lang X, Wang X. Immunization of mice with recombinant S -adenosyl-L-homocysteine hydrolase protein confers protection against Brucella melitensis infection. FEMS Immunol Med Microbiol 2011; 61:159–167 [View Article]
     [Google Scholar]
  59. Zhang Y, Bao H, Miao F, Peng Y, Shen Y et al. Production and application of polyclonal and monoclonal antibodies against Spiroplasma eriocheiris. Sci Rep 2015; 5:17871 [View Article]
     [Google Scholar]
  60. Hans R, Yadav PK, Sharma PK, Boopathi M, Thavaselvam D. Development and validation of immunoassay for whole cell detection of Brucella abortus and Brucella melitensis. Sci Rep 2020; 10:8543 [View Article] [PubMed]
     [Google Scholar]
  61. Abdillahi H, Poolman JT. Whole-cell ELISA for typing Neisseria meningitidis with monoclonal antibodies. FEMS Microbiol Lett 1987; 48:367–371 [View Article]
     [Google Scholar]
  62. Barka N, Tomasi JP, Stadtsbaeder S. Use of whole Streptococcus pneumoniae cells as a solid phase sorbent for C-reactive protein measurement by ELISA. J Immunol Methods 1985; 82:57–63 [View Article] [PubMed]
     [Google Scholar]
  63. Salinovich O, Montelaro RC. Reversible staining and peptide mapping of proteins transferred to nitrocellulose after separation by sodium dodecylsulfate-polyacrylamide gel electrophoresis. Anal Biochem 1986; 156:341–347 [View Article]
     [Google Scholar]
  64. Wang X, Wang Y, Ma L, Zhang R, De Y et al. Development of an improved competitive ELISA based on a monoclonal antibody against lipopolysaccharide for the detection of bovine brucellosis. BMC Vet Res 2015; 11:118 [View Article]
     [Google Scholar]
  65. Olsen SC, Palmer MV. Advancement of knowledge of Brucella over the past 50 years. Vet Pathol 2014; 51:1076–1089 [View Article] [PubMed]
     [Google Scholar]
  66. Gong Q-L, Sun Y-H, Yang Y, Zhao B, Wang Q et al. Global comprehensive literature review and meta-analysis of Brucella spp. in Swine based on publications from 2000 to 2020. Front Vet Sci 2021; 8:630960 [View Article] [PubMed]
     [Google Scholar]
  67. Lipman NS, Jackson LR, Trudel LJ, Weis-Garcia F. Monoclonal versus polyclonal antibodies: distinguishing characteristics, applications, and information resources. ILAR J 2005; 46:258–268 [View Article] [PubMed]
     [Google Scholar]
  68. Byrne B, Stack E, Gilmartin N, O’Kennedy R. Antibody-based sensors: principles, problems and potential for detection of pathogens and associated toxins. Sensors 2009; 9:4407–4445 [View Article] [PubMed]
     [Google Scholar]
  69. Liang H, Zhang L, Fu X, Lin Q, Liu L et al. Development of a double-antibody sandwich ELISA for rapid detection of the MCP antigen concentration in inactivated ISKNV vaccines. Vaccines 2021; 9:1264 [View Article] [PubMed]
     [Google Scholar]
  70. Song D, Qu X, Liu Y, Li L, Yin D et al. A rapid detection method of Brucella with quantum dots and magnetic beads conjugated with different polyclonal antibodies. Nanoscale Res Lett 2017; 12:179 [View Article] [PubMed]
     [Google Scholar]
  71. Estrela P, Sharma S, Byrne H, O’Kennedy RJ. Antibodies and antibody-derived analytical biosensors. Essay Biochemist 2016; 60:9–18 [View Article] [PubMed]
     [Google Scholar]
  72. Jamil T, Khan AU, Saqib M, Hussain MH, Melzer F et al. Animal and human brucellosis in Pakistan. Front Public Health 2021; 9:660508 [View Article] [PubMed]
     [Google Scholar]
  73. Moreno E. Retrospective and prospective perspectives on zoonotic brucellosis. Front Microbiol 2014; 5:213 [View Article] [PubMed]
     [Google Scholar]
  74. Perrett LL, McGiven JA, Brew SD, Stack JA. Evaluation of competitive ELISA for detection of antibodies to Brucella infection in domestic animals. Croat Med J 2010; 51:314–319 [View Article] [PubMed]
     [Google Scholar]
  75. Patra KP, Saito M, Atluri VL, Rolán HG, Young B et al. A protein-conjugate approach to developa monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis. PLoS Negl Trop Dis 2014; 8:e2926 [View Article] [PubMed]
     [Google Scholar]
  76. Islam MRU, Gupta MP, Sidhu PK, Filia G, Saxena HM et al. Comparative evaluation of indirect enzyme linked immunosorbent assay, rose bengal plate test, microagglutination test, and polymerase chain reaction for diagnosis of brucellosis in buffaloes. Turk J Vet Anim Sci 2013; 37:306–310 [View Article]
     [Google Scholar]
  77. Tabasi M, Eybpoosh S, Bouzari S. Development of an indirect ELISA based on whole cell Brucella abortus S99 lysates for detection of IgM anti-Brucella antibodies in human serum. Comp Immunol Microbiol Infect Dis 2019; 63:87–93 [View Article] [PubMed]
     [Google Scholar]
  78. Palmer DA, Douglas JT. Analysis of Brucella lipopolysaccharide with specific and cross-reacting monoclonal antibodies. J Clin Microbiol 1989; 27:2331–2337 [View Article] [PubMed]
     [Google Scholar]
  79. Cloeckaert A, Vizcaíno N, Paquet J-Y, Bowden RA, Elzer PH. Major outer membrane proteins of Brucella spp.: past, present and future. Vet Microbiol 2002; 90:229–247 [View Article] [PubMed]
     [Google Scholar]
  80. Zhu L, He J, Cao X, Huang K, Luo Y et al. Development of a double-antibody sandwich ELISA for rapid detection of Bacillus cereus in food. Sci Rep 2016; 6:16092 [View Article]
     [Google Scholar]
  81. Lim JJ, Kim DH, Lee JJ, Kim DG, Min W et al. Evaluation of recombinant 28 kDa outer membrane protein of Brucella abortus for the clinical diagnosis of bovine brucellosis in Korea. J Vet Med Sci 2012; 74:687–691 [View Article]
     [Google Scholar]
  82. Kaden R, Ferrari S, Jinnerot T, Lindberg M, Wahab T et al. Brucella abortus: determination of survival times and evaluation of methods for detection in several matrices. BMC Infect Dis 2018; 18:259 [View Article] [PubMed]
     [Google Scholar]
  83. Machelart A, Potemberg G, Van Maele L, Demars A, Lagneaux M et al. Allergic asthma favors Brucella growth in the lungs of infected mice. Front Immunol 2018; 9:1856 [View Article]
     [Google Scholar]
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Immunoassay-based evaluation of rOmp28 protein as a candidate for the identification of Brucella species
J. Med. Microbiol. 72, 001718 (2023); https://doi.org/10.1099/jmm.0.001718
/content/journal/jmm/10.1099/jmm.0.001718
/content/journal/jmm/10.1099/jmm.0.001718
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