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Volume 72, Issue 6

Antimicrobial resistance, molecular characteristics, virulence and pathogenicity of -positive Open Access

Abstract

The positive has led to limited therapeutic options for clinical treatment.

Analysing the antimicrobial resistance and molecular typing of -positive is of great significance. Meanwhile, the effect of the gene on the virulence and pathogenicity of remains unclear and should be assessed.

To understand -positive from different perspectives.

The PCR was used to screen -positive , then, antimicrobial susceptibility tests and multilocus sequence typing (MLST) were performed on them; sixty-nine strains of -negative were collected as the controls, 28 pairs of virulence-related genes' carriage and biofilm-forming ability were detected for preliminary evaluation of the virulence phenotype of the strains; to gain insight into the effect of the gene on the virulence and pathogenicity of , the -positive T2 (NDM-1), the T2 knockout strain (ΔNDM-1) and ATCC13047 (ST) were studied, compared the motility, anti-serum killing ability, and virulence to cells. Then, the mice intraperitoneal infection model was established, the survival curve, histopathological characteristics, bacterial load in spleen and the contents of cytokines were compared.

(1) Thirty-five -positive exhibited multidrug resistance. MLST distinguished 12 STs, ST74 was the most common clonal type (11/35), followed by ST114 (10/35). (2) The detection rates of virulence genes , , and in the -positive were significantly higher than those in -negative (<0.05), while there was no significant difference in the amount of biofilm formation between two groups. (3) The presence of gene attenuated the motility diameter of , but had no significant effect on their ability to resist serum killing, and the virulence to cells. The survival rate, histopathological changes, bacterial load in spleen and inflammatory cytokines were not significantly affected.

(1) The -positive exhibited multidrug resistance, and the MLST typing was mainly ST74 and ST114, with a small-scale clonal spread of the ST114 strain in the hospital NICU ward. (2) The gene did not affect the virulence and pathogenicity of .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antimicrobial resistance , blaNDM-1 , Enterobacter cloacae , MLST , pathogenicity and virulence
Funding
This study was supported by the:
  • Yunnan Provincial Science and Technology Department (Award 202001AY070001-037)
    • Principle Award Recipient: MinNiu
  • Scholarship for Academic Leader of Yunnan Province (Award D-2017023)
    • Principle Award Recipient: ShuminLiu
  • Yunnan Provincial Science and Technology Department (Award 2019FE001-060)
    • Principle Award Recipient: YanDu
  • National Natural Science Foundation of China (Award 81960382)
    • Principle Award Recipient: YanDu
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-06-30
2024-05-03
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Antimicrobial resistance, molecular characteristics, virulence and pathogenicity of blaNDM-1-positive Enterobacter cloacae
J. Med. Microbiol. 72, 001712 (2023); https://doi.org/10.1099/jmm.0.001712
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