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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 72, Issue 6

Antimicrobial resistance, molecular characteristics, virulence and pathogenicity of -positive Open Access

Abstract

The positive has led to limited therapeutic options for clinical treatment.

Analysing the antimicrobial resistance and molecular typing of -positive is of great significance. Meanwhile, the effect of the gene on the virulence and pathogenicity of remains unclear and should be assessed.

To understand -positive from different perspectives.

The PCR was used to screen -positive , then, antimicrobial susceptibility tests and multilocus sequence typing (MLST) were performed on them; sixty-nine strains of -negative were collected as the controls, 28 pairs of virulence-related genes' carriage and biofilm-forming ability were detected for preliminary evaluation of the virulence phenotype of the strains; to gain insight into the effect of the gene on the virulence and pathogenicity of , the -positive T2 (NDM-1), the T2 knockout strain (ΔNDM-1) and ATCC13047 (ST) were studied, compared the motility, anti-serum killing ability, and virulence to cells. Then, the mice intraperitoneal infection model was established, the survival curve, histopathological characteristics, bacterial load in spleen and the contents of cytokines were compared.

(1) Thirty-five -positive exhibited multidrug resistance. MLST distinguished 12 STs, ST74 was the most common clonal type (11/35), followed by ST114 (10/35). (2) The detection rates of virulence genes , , and in the -positive were significantly higher than those in -negative (<0.05), while there was no significant difference in the amount of biofilm formation between two groups. (3) The presence of gene attenuated the motility diameter of , but had no significant effect on their ability to resist serum killing, and the virulence to cells. The survival rate, histopathological changes, bacterial load in spleen and inflammatory cytokines were not significantly affected.

(1) The -positive exhibited multidrug resistance, and the MLST typing was mainly ST74 and ST114, with a small-scale clonal spread of the ST114 strain in the hospital NICU ward. (2) The gene did not affect the virulence and pathogenicity of .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antimicrobial resistance , blaNDM-1 , Enterobacter cloacae , MLST , pathogenicity and virulence
Funding
This study was supported by the:
  • Yunnan Provincial Science and Technology Department (Award 202001AY070001-037)
    • Principle Award Recipient: MinNiu
  • Scholarship for Academic Leader of Yunnan Province (Award D-2017023)
    • Principle Award Recipient: ShuminLiu
  • Yunnan Provincial Science and Technology Department (Award 2019FE001-060)
    • Principle Award Recipient: YanDu
  • National Natural Science Foundation of China (Award 81960382)
    • Principle Award Recipient: YanDu
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2023-06-30
2025-01-26
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References

  1. Cai Y, Chen C, Zhao M, Yu X, Lan K et al. High prevalence of metallo-β-lactamase-producing Enterobacter cloacae from Three tertiary hospitals in China. Front Microbiol 2019; 10:1610 [View Article] [PubMed]
     [Google Scholar]
  2. Wang Q, Wang X, Wang J, Ouyang P, Jin C et al. Phenotypic and genotypic characterization of carbapenem-resistant Enterobacteriaceae : data from a longitudinal large-scale CRE study in China (2012–2016). Clin Infect Dis 2018; 67:S196–S205 [View Article]
     [Google Scholar]
  3. Davin-Regli A, Lavigne JP, Pagès JM. Enterobacter spp.: update on taxonomy, clinical aspects, and emerging antimicrobial resistance. Clin Microbiol Rev 2019; 32:00002–00019 [View Article] [PubMed]
     [Google Scholar]
  4. Azevedo PAA, Furlan JPR, Oliveira-Silva M, Nakamura-Silva R, Gomes CN et al. Detection of virulence and β-lactamase encoding genes in Enterobacter aerogenes and Enterobacter cloacae clinical isolates from Brazil. Braz J Microbiol 2018; 49 Suppl 1:224–228 [View Article] [PubMed]
     [Google Scholar]
  5. Mustafa A, Ibrahim M, Rasheed MA, Kanwal S, Hussain A et al. Genome-wide analysis of four Enterobacter cloacae complex type strains: insights into virulence and niche adaptation. Sci Rep 2020; 10:8150 [View Article] [PubMed]
     [Google Scholar]
  6. Brust FR, Boff L, da Silva Trentin D, Pedrotti Rozales F, Barth AL et al. Macrocolony of NDM-1 producing Enterobacter hormaechei subsp. oharae generates subpopulations with different features regarding the response of antimicrobial agents and biofilm formation. Pathogens 2019; 8:49 [View Article] [PubMed]
     [Google Scholar]
  7. Pérez A, Poza M, Fernández A, del Carmen Fernández M, Mallo S et al. Involvement of the AcrAB-TolC efflux pump in the resistance, fitness, and virulence of Enterobacter cloacae. Antimicrob Agents Chemother 2012; 56:2084–2090 [View Article]
     [Google Scholar]
  8. Gygli SM, Borrell S, Trauner A, Gagneux S. Antimicrobial resistance in Mycobacterium tuberculosis: mechanistic and evolutionary perspectives. FEMS Microbiol Rev 2017; 41:354–373 [View Article] [PubMed]
     [Google Scholar]
  9. Fuursted K, Schøler L, Hansen F, Dam K, Bojer MS et al. Virulence of a Klebsiella pneumoniae strain carrying the New Delhi metallo-beta-lactamase-1 (NDM-1). Microbes Infect 2012; 14:155–158 [View Article] [PubMed]
     [Google Scholar]
  10. Yao J, Liu S, Du N, Niu M, Zhang M et al. Genetic features of blandm-1 and characterization of the corresponding knockout mutant of Enterobacter cloacae produced by red homologous recombination. Jundishapur J Microbiol 2020; 13:e101645 [View Article]
     [Google Scholar]
  11. Wu W, Feng Y, Tang G, Qiao F, McNally A et al. NDM metallo-β-lactamases and their bacterial producers in health care settings. Clin Microbiol Rev 2019; 32:e00115–118 [View Article] [PubMed]
     [Google Scholar]
  12. Mezzatesta ML, Gona F, Stefani S. Enterobacter cloacae complex: clinical impact and emerging antibiotic resistance. Future Microbiol 2012; 7:887–902 [View Article] [PubMed]
     [Google Scholar]
  13. Guducuoglu H, Gursoy NC, Yakupogullari Y, Parlak M, Karasin G et al. Hospital outbreak of a colistin-resistant, NDM-1- and OXA-48-producing Klebsiella pneumoniae: high mortality from pandrug resistance. Microb Drug Resist 2018; 24:966–972 [View Article] [PubMed]
     [Google Scholar]
  14. Chavda B, Lv J, Hou M, Chavda KD, Kreiswirth BN et al. Coidentification of Mcr-4.3 and blaNDM-1 in a clinical Enterobacter cloacae isolate from China. Antimicrob Agents Chemother 2018; 62:e00649–18 [View Article]
     [Google Scholar]
  15. Izdebski R, Baraniak A, Herda M, Fiett J, Bonten MJM et al. MLST reveals potentially high-risk international clones of Enterobacter cloacae. J Antimicrob Chemother 2015; 70:48–56 [View Article] [PubMed]
     [Google Scholar]
  16. Wang J, Zhou Z, He F, Ruan Z, Jiang Y et al. The role of the type VI secretion system vgrG gene in the virulence and antimicrobial resistance of Acinetobacter baumannii ATCC 19606. PLoS One 2018; 13:e0192288 [View Article]
     [Google Scholar]
  17. Guérin F, Lallement C, Isnard C, Dhalluin A, Cattoir V et al. Landscape of resistance-nodulation-cell division (RND)-type efflux pumps in Enterobacter cloacae complex. Antimicrob Agents Chemother 2016; 60:2373–2382 [View Article] [PubMed]
     [Google Scholar]
  18. Guérin F, Lallement C, Goudergues B, Isnard C, Sanguinetti M et al. Landscape of in vivo fitness-associated genes of Enterobacter cloacae complex. Front Microbiol 2020; 11:1609 [View Article]
     [Google Scholar]
  19. Huang C, Liu L-Z, Kong H-K, Law COK, Hoa PQ et al. A novel incompatibility group X3 plasmid carrying blaNDM-1 encodes a small RNA that regulates host fucose metabolism and biofilm formation. RNA Biol 2020; 17:1767–1776 [View Article] [PubMed]
     [Google Scholar]
  20. Lee H, Shin J, Chung YJ, Park M, Kang KJ et al. Co-introduction of plasmids harbouring the carbapenemase genes, blaNDM-1 and blaOXA-232, increases fitness and virulence of bacterial host. J Biomed Sci 2020; 27:8 [View Article]
     [Google Scholar]
  21. Göttig S, Riedel-Christ S, Saleh A, Kempf VAJ, Hamprecht A. Impact of blaNDM-1 on fitness and pathogenicity of Escherichia coli and Klebsiella pneumoniae. Int J Antimicrob Agents 2016; 47:430–435 [View Article] [PubMed]
     [Google Scholar]
  22. Gruber TM, Göttig S, Mark L, Christ S, Kempf VAJ et al. Pathogenicity of pan-drug-resistant Serratia marcescens harbouring blaNDM-1. J Antimicrob Chemother 2015; 70:1026–1030 [View Article] [PubMed]
     [Google Scholar]
  23. Zhao Z, Liao C, Chang S, Ding K, Liu Z et al. NDM-1-producing Escherichia coli isolated from pigs induces persistent infection with limited pathogenicity. Microb Pathog 2019; 135:103620 [View Article]
     [Google Scholar]
  24. Almazroo OA, Miah MK, Venkataramanan R. Drug metabolism in the liver. Clin Liver Dis 2017; 21:1–20 [View Article]
     [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.001712
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Antimicrobial resistance, molecular characteristics, virulence and pathogenicity of blaNDM-1-positive Enterobacter cloacae
J. Med. Microbiol. 72, 001712 (2023); https://doi.org/10.1099/jmm.0.001712
/content/journal/jmm/10.1099/jmm.0.001712
/content/journal/jmm/10.1099/jmm.0.001712
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