1887

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Volume 72, Issue 6

Characterization of a strain with high biofilm-forming ability Open Access

Abstract

is highly polymorphic, and some strains are much more likely to cause disease than others. Biofilm formation can help bacteria to survive antibiotic treatment, immune attack and other stresses, promoting persistent infection.

We hypothesized that isolates from patients with more severe associated disease would be better at forming biofilms than isolates from patients with less severe disease.

We initially aimed to determine whether or not the biofilm-forming ability of isolates was associated with disease in the UK-based patients from whom the bacteria were isolated.

Biofilm-forming ability of isolates was determined using a crystal violet assay on glass coverslips. The complete genome sequence of strain 444A was generated by hybrid assembly of Nanopore MinION and Illumina MiSeq data.

Although we found no associations between biofilm-forming ability of and disease severity in patients, we discovered that strain 444A had particularly high biofilm-forming ability. This strain had been isolated from a patient with gastric ulcer disease and moderate to severe scores for induced histopathology. Analysis of the genome of the high biofilm-forming strain 444A revealed that it possesses numerous biofilm- and virulence-associated genes and a small cryptic plasmid encoding a type II toxin–antitoxin system.

There is substantial variation in biofilm-forming ability in but this was not significantly associated with disease severity in our study. We identified and characterized an interesting strain with high biofilm-forming ability, including generation and analysis of the complete genome.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacteria , genome , hybrid assembly , plasmid and toxin-antitoxin
Funding
This study was supported by the:
  • Government of the State of Kuwait
    • Principle Award Recipient: LolwahAlsharaf
  • NIHR Nottingham Biomedical Research Centre
    • Principle Award Recipient: KarenRobinson
© 2023 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-06-09
2024-05-03
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Characterization of a Helicobacter pylori strain with high biofilm-forming ability
J. Med. Microbiol. 72, 001710 (2023); https://doi.org/10.1099/jmm.0.001710
/content/journal/jmm/10.1099/jmm.0.001710
/content/journal/jmm/10.1099/jmm.0.001710
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