1887

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Volume 72, Issue 6

Class 1 integrons in clinical and swine industry isolates of Typhimurium from Colombia, dating 1997 to 2017 No Access

Abstract

subsp. serovar Typhimurium (. Typhimurium) has been linked to outbreaks of foodborne gastroenteritis disease, and the emergence of antimicrobial-resistant clones. In Colombia, laboratory surveillance of spp. between 1997–2018 revealed that . Typhimurium was the most ubiquitous serovar (27.6 % of all isolates), with increasing levels of resistance to several families of antibiotics.

Resistant isolates of . Typhimurium recovered from human clinical, food and swine samples carry class 1 integrons that are linked to antimicrobial resistance genes.

Identify class 1 integrons, and investigate their association with other mobile genetic elements, and their relationship to the antimicrobial resistance of Colombian . Typhimurium isolates.

In this study, 442 isolates of . Typhimurium were analysed, of which 237 were obtained from blood culture, 151 from other clinical sources, 4 from non-clinical sources and 50 from swine samples. Class 1 integrons and plasmid incompatibility groups were analysed by PCR and whole-genome sequencing (WGS), and regions flanking integrons were identified by WGS. The phylogenetic relationship was established by multilocus sequence typing (MLST) and single-nucleotide polymorphism (SNP) distances for 30 clinical isolates.

Overall, 39 % (153/392) of the human clinical isolates and 22 % (11/50) of the swine . Typhimurium isolates carried complete class 1 integrons. Twelve types of gene cassette arrays were identified, including (Int1-Col1), which was the most common one in human clinical isolates (75.2 %, 115/153). Human clinical and swine isolates that carried class 1 integrons were resistant to up to five and up to three antimicrobial families, respectively. The Int1-Col1 integron was most prevalent in stool isolates and was associated with Tn. The most common plasmid incompatibility group was IncA/C.

The widespread presence of the IntI1-Col1 integron in Colombia since 1997 was striking. A possible relationship between integrons, source and mobile elements that favour the spread of antimicrobial resistance determinants in Colombian . Typhimurium was identified.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): drug resistance , integrons , mobile genetic element , plasmid and Salmonella Typhimurium
Funding
This study was supported by the:
  • Ministerio de Agricultura y Desarrollo Rural (Award 20150360)
    • Principle Award Recipient: AnaKarina Carrascal-Camacho
  • Global Challenges Research Fund (Award BBS/OS/GC/000009D)
    • Principle Award Recipient: JayC.D. Hinton
  • Departamento Administrativo de Ciencia, Tecnología e Innovación (COLCIENCIAS) (Award 210471250745)
    • Principle Award Recipient: MagdalenaWiesner
© 2023 The Authors
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Class 1 integrons in clinical and swine industry isolates of Salmonella Typhimurium from Colombia, dating 1997 to 2017
J. Med. Microbiol. 72, 001704 (2023); https://doi.org/10.1099/jmm.0.001704
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