1887

Abstract

One third of people with CF in the UK are co-infected by both and . Chronic bacterial infection in CF contributes to the gradual destruction of lung tissue, and eventually respiratory failure in this group.

The contribution of to cystic fibrosis (CF) lung decline in the presence or absence of is unclear. Defining the molecular and phenotypic characteristics of a range of clinical isolates will help further understand its pathogenic capabilities.

Our objective was to use molecular and phenotypic tools to characterise twenty-five clinical isolates collected from mono- and coinfection with from people with CF at the Royal Victoria Infirmary, Newcastle upon Tyne.

Genomic DNA was extracted and sequenced. Multilocus sequence typing was used to construct phylogeny from the seven housekeeping genes. A pangenome was calculated using Roary, and cluster of Orthologous groups were assigned using eggNOG-mapper which were used to determine differences within core, accessory, and unique genomes. Characterisation of sequence type, clonal complex, and types was carried out using PubMLST, eBURST, AgrVATE and spaTyper, respectively. Antibiotic resistance was determined using Kirby-Bauer disc diffusion tests. Phenotypic testing of haemolysis was carried out using ovine red blood cell agar plates and mucoid phenotypes visualised using Congo red agar.

Clinical strains clustered closely based on type, sequence type and clonal complex. COG analysis revealed statistically significant enrichment of COG families between core, accessory and unique pangenome groups. The unique genome was significantly enriched for replication, recombination and repair, and defence mechanisms. The presence of known virulence genes and toxins were high within this group, and unique genes were identified in 11 strains. Strains which were isolated from the same patient all surpassed average nucleotide identity thresholds, however, differed in phenotypic traits. Antimicrobial resistance to macrolides was significantly higher in the coinfection group.

There is huge variation in genetic and phenotypic capabilities of strains. Further studies on how these may differ in relation to other species in the CF lung may give insight into inter-species interactions.

Funding
This study was supported by the:
  • National Health and Medical Research Council (Award APP2002921)
    • Principle Award Recipient: Jhih-HangJiang
  • Wellcome Trust (Award 224151/Z/21/Z)
    • Principle Award Recipient: TracyPalmer
  • Wellcome Trust (Award 10183/Z/15/Z)
    • Principle Award Recipient: TracyPalmer
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2023-06-08
2024-12-01
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