1887

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Volume 72, Issue 2

Exploration of the inhibitory effect of on quorum sensing mediated virulence factor production and biofilm activity in : an study in model organism No Access

Abstract

Resistance to antibiotics is leading to challenges in the treatment of microbial diseases. One amongst the various approaches to control these pathogens is quorum sensing (QS), which is used to rectify resistance issues. Blocking the bacterial QS circuit is the most reliable anti-virulence therapy to control pathogenicity-associated genes. is a contagious bacterium that proliferates in the host by using signalling molecules like acyl-homoserine lactones; these molecules generate and disseminate toxins and virulence factors for increasing host infection.

The herb is known to have antimicrobial, antidiabetic, anti-inflammatory, antitumor medicinal properties amongst others. We hypothesize that its crude extracts will inhibit the QS circuit of (.

The research work was aimed at evaluating anti-quorum sensing and anti-biofilm activity of various crude extracts from against .

Various extraction methods and solvents were availed for maximum separation, and the extracts were screened for anti-quorum sensing activity. The most potent Fruit Ethyl acetate (FEE) extract at non-inhibitory concentrations was found to interrupt both short-chain (RhlI/R) and long-chain (LasI/R) QS circuits and other virulence factors (<0.05) such as elastase, protease, rhamnolipids and pyocyanin levels in . Biofilm inhibitory properties of FEE were demonstrated using atomic force microscopy, scanning electron microscope and confocal laser microscope. infection model (Paralytic assay) was developed to determine the protective role of FEE by reducing the pathogenicity of .

The study results suggest that hot crude FEE extract interfered in the QS circuit, leading to comprehensive debilitation of QS-controlled virulence factors. The extract reduced virulence factor production in at 4 mg ml concentration whilst paradoxically promoting biofilm formation. Possibly, higher sugar content in the extract promoted clump formation of biofilm architecture by increasing exopolysaccharide production. Moreover, analysis of bacterial pathogenesis on reveals a drastic increase in survival rates in FEE treated worms compared to untreated control.

FEE showed promising QS inhibitory activity against . In the future, additional purification of crude FEE is required to remove carbohydrates, and pure isolated phytochemicals from FEE could be used as therapeutic agents to control QS-mediated infections in .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): biofilm , Cassia fistula , Pseudomonas aeruginosa , quorum sensing and virulence
© 2023 The Authors
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2023-02-14
2024-05-21
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Exploration of the inhibitory effect of Cassia fistula on quorum sensing mediated virulence factor production and biofilm activity in Pseudomonas aeruginosa: an in vivo study in model organism Caenorhabditis elegans
J. Med. Microbiol. 72, 001578 (2023); https://doi.org/10.1099/jmm.0.001578
/content/journal/jmm/10.1099/jmm.0.001578
/content/journal/jmm/10.1099/jmm.0.001578
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