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Abstract

The emergence of antibiotic-resistant isolates is a global concern and has been attributed to the indiscriminate use of antibiotics in humans and animals. Integrons are mobile gene elements closely related to bacterial drug resistance. Among them, class 1 integrons containing various resistance gene cassettes could play an important role in disseminating and maintaining antibiotic resistance in isolates.

. class 1 integrons have a relationship with drug resistance.

. This study aims to investigate the distribution of class 1 integrons and their variable regional molecular characteristics, as well as the diversity of the promoters and drug sensitivity among strains.

. A total of 111 strains, collected between 2018 and 2020, underwent fully automated bacterial identification using the VITEK 2 Compact system and an antibiotic sensitivity test. PCR was employed to screen class 1 integrase genes () and integron variable regions, while promoter type and variable region gene cassette characteristics were determined using sequencing analysis.

. A total of 24 -positive strains were detected in 111 strains. Moreover, -positive strains exhibited statistically significant resistance to ceftazidime, ciprofloxacin, levofloxacin ceftriaxone, trimethoprim/sulfamethoxazole and azithromycin compared to integron-negative strains (<0.05). The multidrug resistance rate of -positive strains was significantly higher than that of negative strains. Variable regions were observed in 6 of the 24 -positive strains. Four gene cassettes were detected, namely , , and . Finally, 3 types of class 1 integron variable region promoters were identified in 24 strains, including PcW, PcH1 and PcW; they are all relatively weak promoters.

. The integron and the drug resistance genes carried by integron have a certain relationship with drug resistance.

Funding
This study was supported by the:
  • Health Commission of Weifang (Award No. wfwsjk-2020-031)
    • Principle Award Recipient: MiLiu
  • Health Commission of Weifang (Award wfwsjk-2020-035)
    • Principle Award Recipient: JieMa
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2022-09-07
2024-12-13
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