1887

Abstract

Carbapenemase-producing (CPE) pose one of the most serious antimicrobial resistance threats to public health worldwide. The outcome of CPE infection differs depending on the resistance mechanism. Therefore, rapid detection of CPE infection is essential for optimizing patient management. The carbapenem inactivation method (CIM) and modified CIM (mCIM) are standard methods for detecting CPE, but they usually require 24 h to generate results.

Recently, an immunochromatographic assay, NG-Test CARBA 5, has become commercially available. It detects the five most common carbapenemase producers (KPC, IMP, NDM, VIM, and OXA-48) rapidly and accurately.

We aimed to evaluate the diagnostic accuracy of NG-Test CARBA 5 for detecting carbapenemase-producing Gram-negative bacilli (CPGNB).

We used 116 carbapenemase-producing strains and 48 non-carbapenemase-producing strains. Of the 116 carbapenemase-producing strains, 107 harboured genes for at least one of the five most common carbapenemases, KPC, IMP, NDM, VIM, and OXA-48-like. Forty-eight non-carbapenemase-producing strains, including carbapenem-resistant , harboured genes for extended-spectrum β-lactamases (CTX-M groups [=25] and SHV groups [=2]) or plasmid-mediated AmpC β-lactamases (DHA [=3], CMY-2 [=2], and CFE-1 [=1]). Antimicrobial susceptibility was tested using the agar dilution method, according to the Clinical and Laboratory Standards Institute guidelines.

Of the 116 carbapenemase-producing strains, 79 were resistant to at least meropenem or imipenem. The sensitivity and specificity of the NG-Test CARBA 5 for the strains were 99.1 % (106 strains positive for 107 strains of the five most common carbapenemase producers) and 100 % (60 strains negative for other types of CPGNB [=10] and non-CPGNB strains [=48]), respectively. The carbapenemase-producing strain with a false-negative result produced IMP-66.

The NG-Test CARBA 5 had high sensitivity and specificity for detecting carbapenemase-producing strains.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.001557
2022-06-07
2022-06-25
Loading full text...

Full text loading...

References

  1. World Health Organization Antimicrobial resistance: global report on surveillance; 2014 http://wwwwhoint/drugresistance/documents/surveillancereport/en
  2. Boutal H, Vogel A, Bernabeu S, Devilliers K, Creton E et al. A multiplex lateral flow immunoassay for the rapid identification of NDM-, KPC-, IMP- and VIM-type and OXA-48-like carbapenemase-producing Enterobacteriaceae. J Antimicrob Chemother 2018; 73:909–915 [View Article] [PubMed]
    [Google Scholar]
  3. Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Susceptibility Testing, Twenty Second Information Wayne, PA, 32 (M100) (Suppl. 22): 2011
    [Google Scholar]
  4. de Lillo A, Ashley FP, Palmer RM, Munson MA, Kyriacou L et al. Novel subgingival bacterial phylotypes detected using multiple universal polymerase chain reaction primer sets. Oral Microbiol Immunol 2006; 21:61–68 [View Article] [PubMed]
    [Google Scholar]
  5. Doi Y, Paterson DL. Carbapenemase-producing Enterobacteriaceae. Semin Respir Crit Care Med 2015; 36:74–84 [View Article]
    [Google Scholar]
  6. Hopkins KL, Meunier D, Naas T, Volland H, Woodford N. Evaluation of the NG-Test CARBA 5 multiplex immunochromatographic assay for the detection of KPC, OXA-48-like, NDM, VIM and IMP carbapenemases. J Antimicrob Chemother 2018; 73:3523–3526 [View Article] [PubMed]
    [Google Scholar]
  7. Komatsu Y, Kasahara K, Inoue T, Lee S-T, Muratani T et al. Molecular epidemiology and clinical features of extended-spectrum beta-lactamase- or carbapenemase-producing Escherichia coli bacteremia in Japan. PLoS One 2018; 13:e0202276 [View Article] [PubMed]
    [Google Scholar]
  8. Poirel L, Walsh TR, Cuvillier V, Nordmann P. Multiplex PCR for detection of acquired carbapenemase genes. Diagn Microbiol Infect Dis 2011; 70:119–123 [View Article] [PubMed]
    [Google Scholar]
  9. Nakano A, Nakano R, Suzuki Y, Saito K, Kasahara K et al. Rapid identification of bla(IMP-1) and bla(IMP-6) by multiplex amplification refractory mutation system PCR. Ann Lab Med 2018; 38:378–380 [View Article] [PubMed]
    [Google Scholar]
  10. Dallenne C, Da Costa A, Decré D, Favier C, Arlet G. Development of a set of multiplex PCR assays for the detection of genes encoding important beta-lactamases in Enterobacteriaceae. J Antimicrob Chemother 2010; 65:490–495 [View Article] [PubMed]
    [Google Scholar]
  11. van der Zwaluw K, de Haan A, Pluister GN, Bootsma HJ, de Neeling AJ et al. The carbapenem inactivation method (CIM), a simple and low-cost alternative for the Carba NP test to assess phenotypic carbapenemase activity in gram-negative rods. PLoS One 2015; 10:e0123690 [View Article] [PubMed]
    [Google Scholar]
  12. Pierce VM, Simner PJ, Lonsway DR, Roe-Carpenter DE, Johnson JK et al. Modified carbapenem inactivation method for phenotypic detection of carbapenemase proCarbapenem Inactivation Method for Phenotypic Detection of Carbapenemase Production among Enterobacteriaceae. J Clin Microbiol 2017; 55:2321–2333 [View Article] [PubMed]
    [Google Scholar]
  13. Takissian J, Bonnin RA, Naas T, Dortet L. NG-Test carba 5 for rapid detection of carbapenemase-producing Enterobacterales from positive blood cCarba 5 for Rapid Detection of Carbapenemase-Producing Enterobacterales from Positive Blood Cultures. Antimicrob Agents Chemother 2019; 63:17–20 [View Article] [PubMed]
    [Google Scholar]
  14. Han R, Guo Y, Peng M, Shi Q, Wu S et al. Evaluation of the immunochromatographic NG-Test Carba 5, RESIST-5 O.O.K.N.V., and IMP K-SeT for Rapid Detection of KPC-, NDM-, IMP-, VIM-type, and OXA-48-like carbapenemase among Enterobacterales. Front Microbiol 2020; 11:609856 [View Article] [PubMed]
    [Google Scholar]
  15. Yonekawa S, Mizuno T, Nakano R, Nakano A, Suzuki Y et al. Molecular and epidemiological characteristics of carbapenemase-producing Klebsiella pneumoniae clinical isolates in Japan. mSphere 2020; 5:1–7 [View Article] [PubMed]
    [Google Scholar]
  16. Lemos EV, de la Hoz FP, Einarson TR, McGhan WF, Quevedo E et al. Carbapenem resistance and mortality in patients with Acinetobacter baumannii infection: systematic review and meta-analysis. Clin Microbiol Infect 2014; 20:416–423 [View Article] [PubMed]
    [Google Scholar]
  17. Baeza LL, Pfennigwerth N, Greissl C, Göttig S, Saleh A et al. Comparison of five methods for detection of carbapenemases in Enterobacterales with proposal of a new algorithm. Clin Microbiol Infect 2019; 25:1286 [View Article] [PubMed]
    [Google Scholar]
  18. Mu X, Nakano R, Nakano A, Ubagai T, Kikuchi-Ueda T et al. Loop-mediated isothermal amplification: Rapid and sensitive detection of the antibiotic resistance gene ISAba1-blaOXA-51-like in Acinetobacter baumannii. J Microbiol Methods 2016; 121:36–40 [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.001557
Loading
/content/journal/jmm/10.1099/jmm.0.001557
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error