1887

Abstract

Tigecycline is an important rescue antibiotic for many bacterial infections. In , tigecycline resistance has been associated with dysregulated stress response caused by aberrations in the interaction of the SigH and RshA factors. In this study, two tigecycline-resistant mutants of (CL5A and CL6A) with mutations in the gene were studied using gene complementation, RT-qPCR and the bacterial adenylate cyclase two-hybrid (BACTH) system. The results supported the premise that mutations in the interrupt the RshA–SigH interaction to cause the overexpression of the gene that leads to tigecycline resistance or reduced susceptibility.

Funding
This study was supported by the:
  • UTARRF (Award IPSR/RMC/UTARRF/2019-C2/T07 (6200/TG6))
    • Principle Award Recipient: ThawZin
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/content/journal/jmm/10.1099/jmm.0.001547
2022-06-14
2022-06-25
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