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Volume 70, Issue 10

The epidemiology of AmpC-producing isolated from dairy cattle faeces on pasture-fed farms Open Access

Abstract

Antibiotic use, particularly amoxicillin-clavulanic acid in dairy farming, has been associated with an increased incidence of AmpC-hyperproducing .

There is limited information on the incidence of AmpC-hyperproducing from seasonal pasture-fed dairy farms.

We undertook a New Zealand wide cross-sectional study to determine the prevalence of AmpC-producing carried by dairy cattle.

Paddock faeces were sampled from twenty-six dairy farms and were processed for the selective growth of both extended-spectrum beta-lactamase (ESBL)- and AmpC-producing . Whole genome sequence analysis was carried out on 35 AmpC-producing .

No ESBL- or plasmid mediated AmpC-producing were detected, but seven farms were positive for chromosomal mediated AmpC-hyperproducing . These seven farms were associated with a higher usage of injectable amoxicillin antibiotics. Whole genome sequence analysis of the AmpC-producing demonstrated that the same strain (<3 SNPs difference) of ST5729 was shared between cows on a single farm. Similarly, the same strain (≤15 SNPs difference) of ST8977 was shared across two farms (separated by approximately 425 km).

These results infer that both cow-to-cow and farm-to-farm transmission of AmpC-producing has occurred.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): AmpC , antimicrobial resistance , dairy and Escherichia coli
Funding
This study was supported by the:
  • Wairarapa Research Fund
    • Principle Award Recipient: SaraAnne Burgess
© 2021 Not applicable
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-10-21
2024-05-11
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The epidemiology of AmpC-producing Escherichia coli isolated from dairy cattle faeces on pasture-fed farms
J. Med. Microbiol. 70, 001447 (2021); https://doi.org/10.1099/jmm.0.001447
/content/journal/jmm/10.1099/jmm.0.001447
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