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Volume 70, Issue 8

Complete genome sequence and probiotic properties of LZys1 isolated from healthy human gut No Access

Abstract

LZys1 ( LZys1) is a type of lactic acid bacteria (LAB), which was initially isolated from healthy human gut.

It was previously anticipated that LZys1 has potential characteristics of probiotic properties. The genetic structure and the regulation functions of LZys1 need to be better revealed.

The aim of this study was to detect the probiotic properties LZys1 and to reveal the genome information related to its genetic adaptation and probiotic profiles.

Multiple experiments were carried out to evaluate its lactic acid-producing ability, resistance to pathogenic bacterial strains, auto-aggregation and co-aggregation ability, and so on. Additionally, complete genome sequencing, gene annotation, and probiotic associated gene analysis were performed.

The complete genome of LZys1 comprised of 1 985 765 bp, with a DNA G+C content of 38.07 %, containing 50 tRNA, seven rRNA, and four sRNA. A total of 1931 genes were classified into six functional categories by Kyoto Encyclopaedia of Genes and Genomes (KEGG) database. The neighbour-joining phylogeny tree based on the whole genome of LZys1 and other probiotics demonstrated that LZys1 has a significant similarity to . The functional genes were detected to expound the molecular mechanism and biochemical processes of its potential probiotic properties, such as gene.

All the results described in this study, together with relevant information previously reported, made LZys1 a very interesting potential strain to be considered as a prominent candidate for probiotic use.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): gene annotation , genome , Lactococcus petauri , molecular mechanism and probiotic properties
Funding
This study was supported by the:
  • the National Students' Platform for Innovation and Entrepreneurship Training Program (Award 201816032014)
    • Principle Award Recipient: OuyangLi
  • Southwest Medical University Science Park (Award 2019005)
    • Principle Award Recipient: yingshunzhou
  • Department of Science and Technology of Sichuan Province (Award 2020YJ0338)
    • Principle Award Recipient: yingshunzhou
  • the National Natural Science Foundation of China (Award 31500114)
    • Principle Award Recipient: yingshunzhou
© 2021 The Authors
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/content/journal/jmm/10.1099/jmm.0.001397
2021-08-16
2024-05-04
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Complete genome sequence and probiotic properties of Lactococcus petauri LZys1 isolated from healthy human gut
J. Med. Microbiol. 70, 001397 (2021); https://doi.org/10.1099/jmm.0.001397
/content/journal/jmm/10.1099/jmm.0.001397
/content/journal/jmm/10.1099/jmm.0.001397
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