1887

Abstract

Non-typeable Haemophilus influenzae (NTHi) is a common opportunistic bacterial pathogen that primarily infects the respiratory mucosa. This study was conducted to assess clinical and microbiological data related to disease severity in patients with lower respiratory tract infections caused by NTHi in a tertiary care hospital in Mexico. NTHi isolates were subjected to serotyping, antimicrobial susceptibility evaluationand analyses of β-lactamase production, genetic relatednessand biofilm formation. Clinical and demographic data were retrieved from patients’ records. The mean age of the patients was 40.3 years; the majority (n=44, 72.1 %) were male. The main comorbidities were arterial hypertension (n=22, 36.1 %) and diabetes mellitus (n=17, 27.9 %). NTHi isolates (n=98) were recovered from tracheal aspirate (n=57, 58.2 %), sputum (n=26, 26.5 %)and bronchial aspirate (n=15, 15.3 %) specimens. Low resistance to cefotaxime (n=0, 0.0 %), rifampin (n=1, 1.1 %) and chloramphenicol (n=3, 3.2 %) and greater resistance to ampicillin (n=30, 32.3 %) and trimethoprim–sulfamethoxazole (n=49, 52.7 %) were detected. β-Lactamase production was found in 17 (17.3 %) isolates. Isolates displayed high genetic diversity, and only 10 (10.2 %) were found to be biofilm producers. The antimicrobial susceptibility patterns of biofilm-producing and non-producing isolates did not differ. Biofilm production was associated with prolonged hospital stay (P=0.05). Lower respiratory NTHi isolates from Mexico showed low antimicrobial resistance and weak biofilm production. Younger age was correlated with lower Acute Physiology and Chronic Health Evaluation II score (moderate, P=0.07; severe, P=0.03).

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2016-12-16
2019-10-15
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References

  1. Alpuche C., Garau J., Lim V..( 2007;). Global and local variations in antimicrobial susceptibilities and resistance development in the major respiratory pathogens. . Int J Antimicrob Agents 30: S135–138. [CrossRef] [PubMed]
    [Google Scholar]
  2. CLSI( 2015a;). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard – Tenth Edition. CLSI document, M07–A10. Wyane, PA, USA:: Clinical and Laboratory Standards Institute.
    [Google Scholar]
  3. CLSI( 2015b;). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement; CLSI document, M100–S25. Wayne, PA, USA:: Clinical and Laboratory Standards Institute.
    [Google Scholar]
  4. Costerton J. W., Stewart P. S., Greenberg E. P..( 1999;). Bacterial biofilms: a common cause of persistent infections. . Science 284: 1318–1322. [CrossRef] [PubMed]
    [Google Scholar]
  5. Duell B. L., Su Y. C., Riesbeck K..( 2016;). Host-pathogen interactions of nontypeable Haemophilus influenzae: from commensal to pathogen. . FEBS Lett, In press, Doi: 10.1002/1873-3468.12351. [CrossRef] [PubMed]
    [Google Scholar]
  6. Jorgensen J. H., Carroll K. C., Pfaller M. A..( 2015;). Manual of Clinical Microbiology. ASM Press;.[Crossref]
    [Google Scholar]
  7. Knaus W. A., Draper E. A., Wagner D. P., Zimmerman J. E..( 1985;). APACHE II: a severity of disease classification system. . Crit Care Med 13: 818–829.[PubMed] [Crossref]
    [Google Scholar]
  8. Langereis J. D., Hermans P. W..( 2013;). Novel concepts in nontypeable Haemophilus influenzae biofilm formation. . FEMS Microbiol Lett 346: 81–89. [CrossRef] [PubMed]
    [Google Scholar]
  9. Mizrahi A., Cohen R., Varon E., Bonacorsi S., Bechet S., Poyart C., Levy C., Raymond J..( 2014;). Non typable-Haemophilus influenzae biofilm formation and acute otitis media. . BMC Infect Dis 14: 400. [CrossRef] [PubMed]
    [Google Scholar]
  10. Murphy T. F., Kirkham C..( 2002;). Biofilm formation by nontypeable Haemophilus influenzae: strain variability, outer membrane antigen expression and role of pili. . BMC Microbiol 2: 7.[PubMed] [Crossref]
    [Google Scholar]
  11. Murphy T. F..( 2003;). Respiratory infections caused by non-typeable Haemophilus influenzae. . Curr Opin Infect Dis 16: 129–134. [CrossRef] [PubMed]
    [Google Scholar]
  12. Murphy T. F., Brauer A. L., Schiffmacher A. T., Sethi S..( 2004;). Persistent colonization by Haemophilus influenzae in chronic obstructive pulmonary disease. . Am J Respir Crit Care Med 170: 266–272. [CrossRef] [PubMed]
    [Google Scholar]
  13. Obaid N. A., Jacobson G. A., Tristram S..( 2015;). Relationship between clinical site of isolation and ability to form biofilms in vitro in nontypeable Haemophilus influenzae. . Can J Microbiol 61: 243–245. [CrossRef] [PubMed]
    [Google Scholar]
  14. Puig C., Calatayud L., Martí S., Tubau F., Garcia-Vidal C., Carratalà J., Liñares J., Ardanuy C..( 2013;). Molecular epidemiology of nontypeable Haemophilus influenzae causing community-acquired pneumonia in adults. . PLoS One 8: e82515. [CrossRef] [PubMed]
    [Google Scholar]
  15. Puig C., Domenech A., Garmendia J., Langereis J. D., Mayer P., Calatayud L., Linares J., Ardanuy C., Marti S..( 2014a;). Increased biofilm formation by nontypeable Haemophilus influenzae isolates from patients with invasive disease or otitis media versus strains recovered from cases of respiratory infections. . Appl Environ Microbiol 80: 7088–7095. [CrossRef]
    [Google Scholar]
  16. Puig C., Marti S., Hermans P. W. M., de Jonge M. I., Ardanuy C., Linares J., Langereis J. D..( 2014b;). Incorporation of phosphorylcholine into the lipooligosaccharide of nontypeable Haemophilus influenzae does not correlate with the level of biofilm formation in vitro. . Infect Immun 82: 1591–1599. [CrossRef]
    [Google Scholar]
  17. Sader H. S., Farrell D. J., Flamm R. K., Jones R. N..( 2014;). Antimicrobial susceptibility of gram-negative organisms isolated from patients hospitalised with pneumonia in US and European hospitals: results from the sentry antimicrobial surveillance program, 2009-2012. . Int J Antimicrob Agents 43: 328–334. [CrossRef] [PubMed]
    [Google Scholar]
  18. Swords W. E..( 2012;). Nontypeable Haemophilus influenzae biofilms: role in chronic airway infections. . Front Cell Infect Microbiol 2: 97. [CrossRef] [PubMed]
    [Google Scholar]
  19. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B..( 1995;). Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. . J Clin Microbiol 33: 2233–2242.[PubMed]
    [Google Scholar]
  20. Tristram S., Jacobs M. R., Appelbaum P. C..( 2007;). Antimicrobial resistance in Haemophilus influenzae. . Clin Microbiol Rev 20: 368–389. [CrossRef] [PubMed]
    [Google Scholar]
  21. Van Eldere J., Slack M. P., Ladhani S., Cripps A. W..( 2014;). Non-typeable Haemophilus influenzae, an under-recognised pathogen. . Lancet Infect Dis 14: 1281–1292. [CrossRef] [PubMed]
    [Google Scholar]
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