1887

Abstract

SUMMARY

Three-day nutrient-broth cultures of gave distinct and reproducible results when tested for haemolysis of sheep RBC. As a medium for detecting haemolysis, heart-infusion broth was no better, and brain-heart-infusion broth was less satisfactory. Lack of reproducibility, and difficulty in interpreting results, were experienced when medium containing glycerol was used and when attempts were made to detect haemolysin in young (8-hour or 1-day) cultures. Horse and human RBC were found too fragile for this test.

Strains of could be separated into three biotypes: classical, El Tor, and an intermediate biotype to which many strains of the present pandemic belong. The latter strains are similar to the El Tor biotype but haemolysin negative. Hence, the haemolysin test, if performed by a reliable method, is a valuable epidemiological tool.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-7-4-521
1974-11-01
2022-12-09
Loading full text...

Full text loading...

/deliver/fulltext/jmm/7/4/medmicro-7-4-521.html?itemId=/content/journal/jmm/10.1099/00222615-7-4-521&mimeType=html&fmt=ahah

References

  1. Barritt M. M. 1936; The intensification of the Voges-Proskauer reaction by the addition of α-naphthol. J. Path. Bact 42:441
    [Google Scholar]
  2. De S. N., Bhattacharyya K., Roychandhury P. K. 1954; The haemolytic activities of Vibrio cholerae and related vibrios. J. Path. Bact 67:117
    [Google Scholar]
  3. Feeley J. C., Pittman M. 1963; Studies on the haemolytic activity of El Tor vibrios. Bull. Wld Hlth Org 28:347
    [Google Scholar]
  4. Greig E. D. W. 1914; The haemolytic action of Indian strains of cholera and cholera-like vibrios. Indian J. med. Res 2:623
    [Google Scholar]
  5. Haddadin J. M., Stirland R. M., Preston N. W., Collard P. 1973; Identification of Vibrio cholerae by pyrolysis-gas-liquid chromatography. Appl. Microbiol 25:40
    [Google Scholar]
  6. Liu P. V. 1959; Studies on the haemolysin of Vibrio cholerae. J. infect. Dis 104:238
    [Google Scholar]
  7. Mukerjee S. 1963; The bacteriophage-susceptibility test in differentiating Vibrio cholerae and Vibrio el tor. Bull. Wld Hlth Org 28:333
    [Google Scholar]
  8. Sakazaki R., Tamura K., Murase M. 1971; Determination of the haemolytic activity of Vibrio cholerae. Jap. J. med. Sci. Biol 24:83
    [Google Scholar]
  9. Sen R., Shrivastava D. L. 1970; A note on designating strains as Vibrio cholerae biotype El tor. Indian J. med Res 58:174
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/00222615-7-4-521
Loading
/content/journal/jmm/10.1099/00222615-7-4-521
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error