1887

Abstract

Summary

Strains of methicillin-resistant (MRSA) from Australia and the UK were compared by digesting their chromosomal DNA with the low-frequency-cutting restriction enzyme I and separating the restriction fragment length polymorphisms (RFLPs) by contour-clamped homogeneous electric field (CHEF) electrophoresis. The numbers of restriction fragments produced were in the range 14–17 and the sizes of the bands were 7–700 kb. Generally, the results confirmed previous conclusions based on antimicrobial resistance and plasmid profiles. The earlier MRSA isolates were different from more recent isolates, and the epidemic MRSA from eastern Australia (EA MRSA) was the same as the epidemic MRSA (EMRSA) found in London hospitals. However, contrary to previous results, the EA MRSA did not constitute a homogeneous group. The results showed that comparison of RFLPs by CHEF electrophoresis is a useful technique for studying the epidemiology of MRSA.

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1992-03-01
2022-01-19
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References

  1. Townsend D. E., Ashdown N., Momoh M., Grubb W. B. Distribution of plasmid-borne resistance to nucleic acid binding compounds in methicillin-resistant Staphylococcus aureus. J Antimicrob Chemother 1985; 15:417–434
    [Google Scholar]
  2. Shanson D. C. Antibiotic resistant Staphylococcus aureus. J Hosp Infect 1981; 2:11–36
    [Google Scholar]
  3. King K., Harvey K. MRSA revisited. Med J Aust 1985; 142:88–89
    [Google Scholar]
  4. Vickery A. M., Beard-Pegler M. A., Rountree P. M. Strain differentiation in methicillin-resistant Staphylococcus aureus. Pathology 1983; 15:235–240
    [Google Scholar]
  5. Richardson J. F., Chittasobhon N., Marples R. R. Supplementary phages for the investigation of strains of methicillin-resistant Staphylococcus aureus. J Med Microbiol 1988; 25:67–74
    [Google Scholar]
  6. Townsend D. E., Ashdown N., Pearman J. W., Annear I., Grubb W. B. Genetics and epidemiology of methicillin-resistant Staphylococcus aureus isolated in a Western Australian hospital. Med J Aust 1985; 142:108–111
    [Google Scholar]
  7. Lacey R. W., Grinsted J. Genetic analysis of methicillin-resistant strains of Staphylococcus aureus; evidence for their evolution from a single clone. J Med Microbiol 1973; 6:511–526
    [Google Scholar]
  8. Townsend D. E., Ashdown N., Bolton S. The international spread of methicillin-resistant Staphylococcus aureus. J Hosp Infect 1987; 9:60–71
    [Google Scholar]
  9. Grubb W. B., Annear D. I. Unstable drug resistance in Staphylococcus aureus M4. Genet Res Camb 1981; 38:217–223
    [Google Scholar]
  10. Udo E., Grubb W. B. Transfer of resistance determinants in a multi-resistant Staphylococcus aureus isolate. J Med Microbiol 1991; 35:72–79
    [Google Scholar]
  11. Jaffe H. W., Sweeney H. M., Nathan C., Weinstein R. A., Kabins S. A., Cohen S. Identity and interspecific transfer of gentamicin-resistance plasmids in Staphylococcus aureus and Staphylococcus epidermidis. J Infect Dis 1980; 141:738–747
    [Google Scholar]
  12. Kerr S., Kerr G. E., Mackintosh C. A., Marples R. R. A survey of methicillin-resistant Staphylococcus aureus affecting patients in England and Wales. J Hosp Infect 1990; 16:35–48
    [Google Scholar]
  13. Branger C. Goullet, Ph., Genetic heterogeneity in methicillin-resistant strains of Staphylococcus aureus revealed by esterase electrophoretic polymorphism. J Hosp Infect 1989; 14:125–134
    [Google Scholar]
  14. Stephenson J. R., Cook S. J., Tabaqchali S. New method fortyping Staphylococcus aureus resistant to methicillin based on sulphur-35 methionine labelled proteins: its application in an outbreak. Br Med J 1986; 293:581–583
    [Google Scholar]
  15. Gaston M. A., Duff P. S., Naidoo J. Evaluation of electrophoretic methods for typing methicillin-resistant Staphylococcus aureus. J Med Microbiol 1988; 26:189–197
    [Google Scholar]
  16. Kreiswirth B. N., McGeer A., Komblum J. The use of variable gene probes to investigate a multihospital outbreak of MRSA. In Novick R. (ed) Molecular biology of the staphylococci New York: VCH Publishers; 1990521–530
    [Google Scholar]
  17. Hadorn K., Lenz W., Kayser F. H., Shalit I., Krasemann C. Use of a ribosomal RNA gene probe for the epidemiologic study of methicillin and ciprofloxacin resistant Staphylococcus aureus. Eur J Clin Microbiol Infect Dis 1990; 9:649–653
    [Google Scholar]
  18. Burnie J. P., Matthews R. C., Lee W., Murdoch D. A comparison of immunoblot and DNA restriction patterns in characterising methicillin-resistant isolates of Staphylococcus aureus. J Med Microbiol 1989; 29:255–261
    [Google Scholar]
  19. Jordens J. Z., Hall L. M. C. Characterisation of methicillin-resistant Staphylococcus aureus isolates by restriction endonuclease digestion of chromosomal DNA. J Med Microbiol 1988; 27:117–123
    [Google Scholar]
  20. Owen R. J. Chromosomal DNA fingerprinting—a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol 1989; 30:89–99
    [Google Scholar]
  21. Schwartz D. C., Cantor C. R. Separation of yeast chromosome sized DNAs by pulsed field gradient gel electrophoresis. Cell 1984; 37:67–75
    [Google Scholar]
  22. Vollrath D., Davis R. W. Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields. Nucleic Acids Res 1987; 15:7865–7876
    [Google Scholar]
  23. Carle G. F., Frank M., Olson M. V. Electrophoretic separations of large DNA molecules by periodic inversion of the electric field. Science 1986; 232:65–68
    [Google Scholar]
  24. Fan J.-B., Chikashige Y., Smithm C. L., Niwa O., Yanagida M., Cantor C. R. Construction of a NotI restriction map of the fission yeast Schizosaccharomyces pombe genome. Nucleic Acids Res 1988; 17:2801–2818
    [Google Scholar]
  25. Orbach M. J., Vollrath D., Davis R. W., Yanofsky C. An electrophoretic karyotype of Neurospora crassa. Mol Cell Biol 1988; 8:1469–1473
    [Google Scholar]
  26. Smith C. L., Klco S., Cantor C. R. Pulsed field gel electrophoresis and technology of large DNA molecules. In Davies K. E. (ed) Genome analysis: a practical approach Oxford: IRL Press; 198841–72
    [Google Scholar]
  27. Smith C. L., Econome J. G., Schutt A., Klco S., Cantor C. R. A physical map of the Escherichia coli K12 genome. Science 1987; 236:1448–1453
    [Google Scholar]
  28. Kauc L., Mitchell M., Goodgal S. H. Size and physical map of the chromosome of Haemophilus influenzae. J Bacteriol 1989; 171:2474–2479
    [Google Scholar]
  29. Grothues D., Tümmler B. Genome analysis of Pseudomonas aeruginosa by field inversion gel electrophoresis. FEMS Microbiol Lett 1987; 48:419–422
    [Google Scholar]
  30. Goering R. V., Duensing T. D. Rapid field inversion gel electrophoresis in combination with an rRNA gene probe in the epidemiological evaluation of staphylococci. J Clin Microbiol 1990; 28:426–429
    [Google Scholar]
  31. Townsend D. E., Grubb W. B., Ashdown N. Genetics of drug resistance in methicillin-resistant Staphylococcus aureus from Australian hospitals. J Hosp Infect 1983; 4:331–337
    [Google Scholar]
  32. Dice L. R. Measures of the amount of ecologic association between species. Ecology 1945; 26:297–302
    [Google Scholar]
  33. Grubb W. B., Townsend D. E., Greed L., Ashdown N., Momoh M. Characteristics of methicillin-resistant Staphylococcus aureus endemic in Australian hospitals. In Spitzy K. H., Karrer K. (eds) Proceedings of the 13th international congress of chemotherapy Vienna, part 83 1983401–402
    [Google Scholar]
  34. Lyon B. R., Iuorio J. L., May J. W., Skurray R. A. Molecular epidemiology of multiresistant Staphylococcus aureus in Australian hospitals. J Med Microbiol 1984; 17:79–89
    [Google Scholar]
  35. Wei M.-Q., Groth D. M., Mendis A. H. W. Typing of methicillin-resistant Staphylococcus aureus with a M13 repeat probe. J Hosp Infect 1992 in press
    [Google Scholar]
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