1887

Abstract

Summary.

Chemotaxis of polymorphonuclear leukocytes (PMNL) in response to cell components of alone or in combination with was evaluated. . produced much more powerful chemotactic factors than . The culture filtrate (CF), outer membrane (OM) preparation, and lipopolysaccharide (LPS) of . slightly stimulated chemotactic activity of PMNL. The culture filtrate and OM preparation were capable of inhibiting the chemotaxis of PMNL in response to the chemotactic factors of . but LPS of . was not able to do so. Reduction by B. fragilis of PMNL chemotaxis in response to . was not specific for . but also occurred in the presence of facultative bacteria. In parallel with chemotaxis, lysozyme release, but not -glucuronidase release, by PMNL was significantly stimulated by . but not by . .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/00222615-24-2-119
1987-09-01
2024-11-09
Loading full text...

Full text loading...

/deliver/fulltext/jmm/24/2/medmicro-24-2-119.html?itemId=/content/journal/jmm/10.1099/00222615-24-2-119&mimeType=html&fmt=ahah

References

  1. Adamu S A, Sperry J F. 1981; Polymorphonuclear neutrophil chemotaxis induced and inhibited by Bacteroides spp. Infection and Immunity 33:806–810
    [Google Scholar]
  2. Becker E L, Showell H J. 1974; The ability of chemotactic factors to induce lysosomal enzyme release. 2 The mechanism of release. Journal of Immunology 112:2055–2062
    [Google Scholar]
  3. Becker E L. 1976; Some interrelations of neutrophil chemotaxis, lysosomal enzyme secretion, and phagocytosis as revealed by synthetic peptides. American Journal of Pathology 85:385–394
    [Google Scholar]
  4. Boehringer Mannheim Co; 1981 Biochemical information. Boehringer Mannheim Co; Biochemica, West Germany:
    [Google Scholar]
  5. Connolly J C, McLean C, Tabaqchali S. 1984; The effect of capsular polysaccharide and lipopolysaccharide of Bacteroides fragilis on polymorph function and serum killing. Journal of Medical Microbiology 17:259–271
    [Google Scholar]
  6. Davis A T, Estensen R, Quie P G. 1971; Cytochalasin B.3. Inhibition of human polymorphonuclear leukocyte phagocytosis. Proceedings of the Society for Experimental Biology and Medicine 137:161–164
    [Google Scholar]
  7. Gorbach S L, Bartlett J G. 1974; Anaerobic infections. New England Journal of Medicine 290:1177–1184 1237–1245,1287–1289–1294
    [Google Scholar]
  8. Hofstad T, Kristoffersen T. 1970; Chemical characteristics of endotoxin from Bacteroides fragilis NCTC 9343. Journal of General Microbiology 61:15–19
    [Google Scholar]
  9. Ingham H R, Sisson P R, Tharagonnet D, Selkon J B, Codd A. 1977; Inhibition of phagocytosis in vitro by obligate anaerobes. Lancet1252–1254
    [Google Scholar]
  10. Jones G R, Gemmell C G. 1982; Impairment by Bacteroides species of opsonisation and phagocytosis of enterobacteria. Journal of Medical Microbiology 15:351–361
    [Google Scholar]
  11. Kasper D L, Seiler M W. 1975; Immunochemical characterization of the outer membrane complex of Bacteroides fragilis subspecies fragilis. Journal of Infectious Diseases 132:440–450
    [Google Scholar]
  12. Kasper D L. 1976; Chemical and biological characterization of the lipopolysaccharide of Bacteroides fragilis subspecies fragilis . Journal of Infectious Diseases 134:59–66
    [Google Scholar]
  13. Keleti G, Lederer W H. 1974; Handbook of micro-methods for the biological sciences. Van Nostrand-Reinhold Co.; New York:3–4–31–32
    [Google Scholar]
  14. MacLaren D M, Namavar F, Verweij-van Vught A M J J, Vel W A C, Kaan J A. 1984; Pathogenic synergy: mixed intra-abdominal infections. Antonie van Leeuwenhoek 50:775–787
    [Google Scholar]
  15. Namavar F, Verweij-van Vught A M J J, Bal M, van Steenbergen T J M, de Graaff J, MacLaren D M. 1983; Effect of anaerobic bacteria on killing of Proteus mirabilis by human polymorphonuclear leukocytes. Infection and Immunity 40:930–935
    [Google Scholar]
  16. Namavar F, Verweij-van Vught A M J J, Vel W A C, Bal M, MacLaren D M. 1984; Polymorphonuclear leukocyte chemotaxis by mixed anaerobic and aerobic bacteria. Journal of Medical Microbiology 18:167–172
    [Google Scholar]
  17. Shah H N, Williams R A D, Bowden G H, Hardie J M. 1976; Comparison of the biochemical properties of Bacteroides melaninogenicus from human dental plaque and other sites.. Journal of Applied Bacteriology 41:473–492
    [Google Scholar]
  18. Showell H J. 1976; The structure-activity relations of synthetic peptides as chemotactic factors and inducers of lysosomal enzyme secretion for neutrophils. Journal of Experimental Medicine 143:1154–1169
    [Google Scholar]
  19. Smith R, Wierenga W, Iden S S. 1980; Characteristics of N-formyl-methionyl-leucyl-phenylalanine as an inducer of lysosomal enzyme release from human neutrophils. Inflammation 4:73–88
    [Google Scholar]
  20. Sveen K, Hofstad T, Milner K C. 1977; Lethality for mice and chick embryos, pyrogenicity in rabbits, and ability to gelate lysate from amoebocytes from Limulus polyphemus by lipopolysaccharides from Bacteroides Fusobacterium and Veillonella . Acta Pathologica et Microbiologica Scandinavica Section 5:85388–396
    [Google Scholar]
  21. Varel V H, Bryant M P. 1974; Nutritional features of Bacteroides fragilis subsp.fragilis . Applied Microbiology 18:251–257
    [Google Scholar]
  22. Veale D R, Smith H, Witt K A, Marshall R B. 1974; Differential ability of colonial types of Neisseria gonorrhoeae to produce infection and an inflammatory response in sub-cutaneous perforated plastic chambers in Guinea-pigs and rabbits. Journal of Medical Microbiology 8:325–335
    [Google Scholar]
  23. Vel W A C, Namavar F, Verweij-van Vught A M J J, Pubben A N B, MacLaren D M. 1985; Killing of Escherichia coli by human polymorphonuclear leucocytes in the presence ofBacteroides fragilis . Journal of Clinical Pathology 38:86–91
    [Google Scholar]
  24. Verweij-van Vught A M J J, Namavar F, Sparrius M, Vel W A C, MacLaren D M. 1985; Pathogenic synergy between Escherichia coli and Bacteroides fragilis: studies in an experimental mouse model. Journal of Medical Microbiology 19:325–331
    [Google Scholar]
  25. Wade B H, Kasper D L, Mandell G L. 1983; Interactions of Bacteroides fragilis and phagocytes: studies with whole organisms, purified capsular polysaccharide and clindamy-cin-treated bacteria. Journal of Antimicrobial Chemotherapy 12:51–62
    [Google Scholar]
/content/journal/jmm/10.1099/00222615-24-2-119
Loading
/content/journal/jmm/10.1099/00222615-24-2-119
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error