1887

Abstract

Summary

A modification of the indirect haemagglutination test is described for the serodiagnosis of echinococcosis. In the modified test the Cowan I strain of , which contains protein A, was used to enhance haemagglutination of sensitised red cells. The test was performed in parallel with the indirect haemagglutination test on 31 sera from surgically-confirmed cases of hydatid disease and on 45 sera from healthy blood donors. Use of protein A enhanced the sensitivity of the test and greatly increased the titres obtained with most of the sera. None of the sera from healthy blood donors exhibited a titre > 64, whereas all but two of the sera from cases of hydatid disease exhibited titres > 128. The immunoassay is simple, inexpensive and requires little technical skill. It has the potential for wide application in the serodiagnosis of echinococcosis.

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1986-11-01
2022-05-22
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References

  1. Arabatzis G., Papapanagiotou R. 1963; Laboratory tests in hydatid disease: a comparison of the indirect haemagglutination, complement-fixation and intradermal tests. Bulletin of the World Health Organization 28:266–268
    [Google Scholar]
  2. Ardehali S., Kohanteb D. J., Gerami S., Behfourouz N., Rezai H. R., Vaez-Zadeh K. 1977; Evaluation of counter immuno-electrophoresis, crossed electro-immunodiffusion and agar gel diffusion for immunodiagnosis of human hydatid disease. Transactions of the Royal Society of Tropical Medicine and Hygiene 71:481–485
    [Google Scholar]
  3. Bergquist N. R., Waller T. 1983; A novel simple immunoassay for rapid detection of human IgG antibodies to Toxoplasma gondii . Journal of Immunological Methods 61:339–344
    [Google Scholar]
  4. Camargo E. P., Mattet D. M., Yoshida N., Caulada Z. 1983; Staphylococci adherence to trypanosomes exposed to immune sera as a method for the diagnosis of Chagas’ disease. Transactions of the Royal Society of Tropical Medicine and Hygiene 77:825–827
    [Google Scholar]
  5. Chemtai A. K., Bowry T. R., Ahmad Z. 1981; Evaluation of five immunodiagnostic techniques in echinococcosis patients. Bulletin of the World Health Organization 59:767–772
    [Google Scholar]
  6. Coombs R. R. A., Howard A. N., Mynors L. S. 1953; A serological procedure theoretically capable of detecting incomplete or non-precipitating antibodies to soluble protein antigens. British Journal of Experimental Pathology 34:525–534
    [Google Scholar]
  7. Farshy Kagan I. G. 1972; Use of stable sensitized cells in indirect microhemagglutination test for malaria. American Journal of Tropical Medicine and Hygiene 21:868–872
    [Google Scholar]
  8. Iacona A., Pini C., Vicari G. 1980; Enzyme-Linked immunosorbent assay (ELISA) in the serodiagnosis of hydatid disease. American Journal of Tropical Medicine and Hygiene 29:95–102
    [Google Scholar]
  9. Jagannath C., Sengupta D. N. 1981; Serology of leprosy. I. Indirect haemagglutination test with stabilised sensitised red cells. Leprosy in India 53:507–512
    [Google Scholar]
  10. Jagannath C., Sengupta D. N., Kasinathan S. 1984; Staphylococcus aureus protein A antibody-mediated haemagglutination assay. A study on the interaction between protein A-bound specific antibodies and red cell bound specific antigens. International Archives of Allergy and Applied Immunology 73:296–302
    [Google Scholar]
  11. Kelkar Kotwal S. E. 1975; Counterimmunoelectrophoresis in diagnosis of hydatid disease. Lancet i:755–756
    [Google Scholar]
  12. Kronvall G. 1973; A rapid slide-agglutination method for typing pneumococci by means of specific antibody adsorbed to protein A-containing staphylococci. Journal of Medical Microbiology 6:187–190
    [Google Scholar]
  13. Langone J. J. 1982; Use of labeled protein A in quantitative immunochemical analysis of antigens and antibodies. Journal of Immunological Methods 51:3–22
    [Google Scholar]
  14. Lewis J. W., Kerstein M. D. 1975; A review of echinococcal disease. Annals of Surgery 181:390–396
    [Google Scholar]
  15. Mansueto S., Migneco G., Tripi S., Picone D. M. 1980; Simplified counter-immuno-electrophoresis (CIEP) with a commercially produced antigen on cellulose acetate membrane for the diagnosis of hydatidosis. Transactions of the Royal Society of Tropical Medicine and Hygiene 74:260–261
    [Google Scholar]
  16. Ouchterlony O. 1958; Diffusion-in-gel methods for immunological analysis. Progress in Allergy 5:1–78
    [Google Scholar]
  17. Parija S. C., Ananthakrishnan N. 1985; Evaluation of stabilised cells in the Indirect haemagglutination test for echinococcosis. Journal of Medical Microbiology 19:95–98
    [Google Scholar]
  18. Parija S. C., Mishra S. R., RaoSambasiva R. 1986; Sensitised chick cells in IHA for echinococcosis. Journal of Medical Microbiology 22:243–245
    [Google Scholar]
  19. Parija S. C., Rao R. S., Badrinath S., Sengupta D. N. 1983; Hydatid disease in Pondicherry. Journal of Tropical Medicine and Hygiene 86:113–115
    [Google Scholar]
  20. Schantz P. M., Pappas M. G., Cannon L. T., Wahlquist S. P. 1984; Development of the Dot-ELISA for serodiagnosis of hydatid disease. Federation Proceedings 43:1515
    [Google Scholar]
  21. Sivadasan K., Kurien B., John T. J. 1984; Rapid diagnosis of typhoid fever by antigen detection. Lancet i:134–135
    [Google Scholar]
  22. Stavitsky A. B. 1954; Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. Journal of Immunology 72:360–367
    [Google Scholar]
  23. Varela-Diaz V. M., Coltori E. A. 1974; Technicas para el diagnostico immunologica de la hidatidosis humana. Centro Panamericano de Zoonosis, Series de Monografias Cientificas y Technicas C.P.Z No. 7, Buenos Aires
    [Google Scholar]
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