1887

Abstract

Singapore grouper iridovirus (SGIV), an iridovirus in the genus , is a major pathogen that results in significant economic losses in grouper aquaculture. To investigate further its infective mechanisms, for the first time, a viral DNA microarray was generated for the SGIV genome to measure the expression of its predicted open reading frames simultaneously . By using the viral DNA microarray, the temporal gene expression of SGIV was characterized and the DNA microarray data were consistent with the results of real-time RT-PCR studies. Furthermore, different-stage viral genes (i.e. immediate-early, early and late genes) of SGIV were uncovered by combining drug treatments and DNA microarray studies. These results should offer important insights into the replication and pathogenesis of iridoviruses.

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2006-10-01
2019-10-14
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vol. , part 10, pp. 2907 – 2915

SGIV primers

Partial cDNA sequences of β-actin and GAPDH

Primers for real-time PCR

SGIV genes with temporal expression on array

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