1887

Abstract

(HCoV-OC43) causes acute, self-limited respiratory infections. A close relationship between bovine coronaviruses (BCoVs) and HCoV-OC43 has recently been demonstrated. This study includes seven clinical, non-cell culture-adapted, contemporary HCoV-OC43 strains detected in France in 2003. By using RT-PCR and clonal sequencing of the S1 gene of HCoV-OC43, the inter-variant heterogeneity of the HCoV-OC43 circulating strains was studied and the intra-variant diversity was assessed by investigation of a quasispecies cloud. This paper brings to the forefront a high genetic diversity of circulating HCoV-OC43 variants. Genetically different groups are defined among the variants described in this study. One of these variants holds characteristics of an outlier and presents a deletion of 12 nt, also found in BCoV strains. Moreover, the presence of HCoV-OC43 as a quasispecies cloud during an acute respiratory-tract illness was discovered. It has also been revealed that quasispecies-cloud sizes are similar for the two viral populations tested.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.82065-0
2006-11-01
2020-10-22
Loading full text...

Full text loading...

/deliver/fulltext/jgv/87/11/3349.html?itemId=/content/journal/jgv/10.1099/vir.0.82065-0&mimeType=html&fmt=ahah

References

  1. Arbour N., Ray D., Newcombe J., Talbot P. J. 2000; Neuroinvasion by human respiratory coronaviruses. J Virol 74:8913–8921 [CrossRef]
    [Google Scholar]
  2. Arias A., Lázaro E., Escarmís C., Domingo E. 2001; Molecular intermediates of fitness gain of an RNA virus: characterization of a mutant spectrum by biological and molecular cloning. J Gen Virol 82:1049–1060
    [Google Scholar]
  3. Cavanagh D. 1995; The coronavirus surface glycoprotein. In The Coronaviridae pp  73–113 Edited by Siddell S. G. New York: Plenum;
    [Google Scholar]
  4. Cavanagh D. 1997; Nidovirales : a new order comprising Coronaviridae and Arteriviridae . Arch Virol 142:629–633
    [Google Scholar]
  5. Domingo E., Escarmís C., Sevilla N., Moya A., Elena S. F., Quer J., Novella I. S., Holland J. J. 1996; Basic concepts in RNA virus evolution. FASEB J 10:859–864
    [Google Scholar]
  6. Gallagher T. M., Buchmeier M. J. 2001; Coronavirus spike proteins in viral entry and pathogenesis. Virology 279:371–374 [CrossRef]
    [Google Scholar]
  7. Grandadam M., Tebbal S., Caron M., Siriwardana M., Larouze B., Koeck J. L., Buisson Y., Enouf V., Nicand E. 2004; Evidence for hepatitis E virus quasispecies. J Gen Virol 85:3189–3194 [CrossRef]
    [Google Scholar]
  8. Hall T. A. 1999; BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98
    [Google Scholar]
  9. Jeong J.-H., Kim G.-Y., Yoon S.-S. & 9 other authors 2005; Molecular analysis of S gene of spike glycoprotein of winter dysentery bovine coronavirus circulated in Korea during 2002–2003. Virus Res 108:207–212 [CrossRef]
    [Google Scholar]
  10. Künkel F., Herrler G. 1993; Structural and functional analysis of the surface protein of human coronavirus OC43. Virology 195:195–202 [CrossRef]
    [Google Scholar]
  11. Sánchez G., Bosch A., Gómez-Mariano G., Domingo E., Pintó R. M. 2003; Evidence for quasispecies distributions in the human hepatitis A virus genome. Virology 315:34–42 [CrossRef]
    [Google Scholar]
  12. Smith D. B., McAllister J., Casino C., Simmonds P. 1997; Virus ‘quasispecies’: making a mountain out of a molehill?. J Gen Virol 78:1511–1519
    [Google Scholar]
  13. Swofford D. L. 2003 paup*: phylogenetic analysis using parsimony (*and other methods), version 4 Sunderland, MA: Sinauer Associates;
    [Google Scholar]
  14. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [CrossRef]
    [Google Scholar]
  15. Vignuzzi M., Stone J. K., Arnold J. J., Cameron C. E., Andino R. 2006; Quasispecies diversity determines pathogenesis through cooperative interactions in a viral population. Nature 439:344–348 [CrossRef]
    [Google Scholar]
  16. Vijgen L., Keyaerts E., Moës E., Thoelen I., Wollants E., Lerney P., Vandamme A.-M., Van Ranst M. 2005a; Complete genomic sequence of human coronavirus OC43: molecular clock analysis suggests a relatively recent zoonotic coronavirus transmission event. J Virol 79:1595–1604 [CrossRef]
    [Google Scholar]
  17. Vijgen L., Keyaerts E., Lerney P., Moës E., Li S., Vandamme A.-M., Van Ranst M. 2005b; Circulation of genetically distinct contemporary human coronavirus OC43 strains. Virology 337:85–92 [CrossRef]
    [Google Scholar]
  18. Wang W.-K., Lin S.-R., Lee C.-M., King C.-C., Chang S.-C. 2002; Dengue type 3 virus in plasma is a population of closely related genomes: quasispecies. J Virol 76:4662–4665 [CrossRef]
    [Google Scholar]
  19. Zhang X. M., Herbst W., Kousoulas K. G., Storz J. 1994; Biological and genetic characterization of a hemagglutinating coronavirus isolated from a diarrhoeic child. J Med Virol 44:152–161 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.82065-0
Loading
/content/journal/jgv/10.1099/vir.0.82065-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error