1887

Abstract

We have created a completely helper cell-dependent morbillivirus by modifying the genome to remove the coding sequence of the phosphoprotein (P) and recovering the recombinant virus in a cell line constitutively expressing the P protein. The P protein-deleted virus (P) grew very inefficiently unless both of the viral accessory proteins (V and C) were also expressed. Growth of the virus was restricted to the P-expressing cell line. The P virus grew more slowly than the parental virus and expressed much less viral protein in infected cells. The technique could be used to create virus-like particles for use as a vaccine or as antigen in immunological or serological assays.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/vir.0.050872-0
2013-06-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/94/6/1195.html?itemId=/content/journal/jgv/10.1099/vir.0.050872-0&mimeType=html&fmt=ahah

References

  1. Anderson J. , McKay J. A. . ( 1994; ). The detection of antibodies against peste des petits ruminants virus in cattle, sheep and goats and the possible implications to rinderpest control programmes. . Epidemiol Infect 112:, 225–231. [CrossRef] [PubMed]
    [Google Scholar]
  2. Anderson J. , McKay J. A. , Butcher R. N. . ( 1990; ). The use of monoclonal antibodies in competitive ELISA for the detection of antibodies to rinderpest and peste des petits ruminants viruses. . In Seromonitoring of Rinderpest throughout Africa: Phase One Proceedings of the Final Research Coordination Meeting of the IAEA Rinderpest Control Projects, Cote d’Ivoire 19–23 November 1990 IAEA-TECDOC-623. Vienna:: International Atomic Energy Agency;.
    [Google Scholar]
  3. Baron M. D. , Barrett T. . ( 1997; ). Rescue of rinderpest virus from cloned cDNA. . J Virol 71:, 1265–1271.[PubMed]
    [Google Scholar]
  4. Baron M. D. , Barrett T. . ( 2000; ). Rinderpest viruses lacking the C and V proteins show specific defects in growth and transcription of viral RNAs. . J Virol 74:, 2603–2611. [CrossRef] [PubMed]
    [Google Scholar]
  5. Baron M. D. , Foster-Cuevas M. , Baron J. , Barrett T. . ( 1999; ). Expression in cattle of epitopes of a heterologous virus using a recombinant rinderpest virus. . J Gen Virol 80:, 2031–2039.[PubMed]
    [Google Scholar]
  6. Brown D. D. , Collins F. M. , Duprex W. P. , Baron M. D. , Barrett T. , Rima B. K. . ( 2005; ). ‘Rescue’ of mini-genomic constructs and viruses by combinations of morbillivirus N, P and L proteins. . J Gen Virol 86:, 1077–1081. [CrossRef] [PubMed]
    [Google Scholar]
  7. Cathomen T. , Mrkic B. , Spehner D. , Drillien R. , Naef R. , Pavlovic J. , Aguzzi A. , Billeter M. A. , Cattaneo R. . ( 1998; ). A matrix-less measles virus is infectious and elicits extensive cell fusion: consequences for propagation in the brain. . EMBO J 17:, 3899–3908. [CrossRef] [PubMed]
    [Google Scholar]
  8. Cattaneo R. , Kaelin K. , Baczko K. , Billeter M. A. . ( 1989; ). Measles virus editing provides an additional cysteine-rich protein. . Cell 56:, 759–764. [CrossRef] [PubMed]
    [Google Scholar]
  9. Gassen U. , Collins F. M. , Duprex W. P. , Rima B. K. . ( 2000; ). Establishment of a rescue system for canine distemper virus. . J Virol 74:, 10737–10744. [CrossRef] [PubMed]
    [Google Scholar]
  10. Halfmann P. , Kim J. H. , Ebihara H. , Noda T. , Neumann G. , Feldmann H. , Kawaoka Y. . ( 2008; ). Generation of biologically contained Ebola viruses. . Proc Natl Acad Sci U S A 105:, 1129–1133. [CrossRef] [PubMed]
    [Google Scholar]
  11. Hu Q. , Chen W. , Huang K. , Baron M. D. , Bu Z. . ( 2012; ). Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test. . Vet Res 43:, 48. [CrossRef] [PubMed]
    [Google Scholar]
  12. Mahapatra M. , Parida S. , Baron M. D. , Barrett T. . ( 2006; ). Matrix protein and glycoproteins F and H of Peste-des-petits-ruminants virus function better as a homologous complex. . J Gen Virol 87:, 2021–2029. [CrossRef] [PubMed]
    [Google Scholar]
  13. Mühlberger E. , Weik M. , Volchkov V. E. , Klenk H. D. , Becker S. . ( 1999; ). Comparison of the transcription and replication strategies of Marburg virus and Ebola virus by using artificial replication systems. . J Virol 73:, 2333–2342.[PubMed]
    [Google Scholar]
  14. Nakatsu Y. , Takeda M. , Ohno S. , Shirogane Y. , Iwasaki M. , Yanagi Y. . ( 2008; ). Measles virus circumvents the host interferon response by different actions of the C and V proteins. . J Virol 82:, 8296–8306. [CrossRef] [PubMed]
    [Google Scholar]
  15. Nanda S. K. , Baron M. D. . ( 2006; ). Rinderpest virus blocks type I and type II interferon action: role of structural and nonstructural proteins. . J Virol 80:, 7555–7568. [CrossRef] [PubMed]
    [Google Scholar]
  16. Ohno S. , Ono N. , Takeda M. , Takeuchi K. , Yanagi Y. . ( 2004; ). Dissection of measles virus V protein in relation to its ability to block alpha/beta interferon signal transduction. . J Gen Virol 85:, 2991–2999. [CrossRef] [PubMed]
    [Google Scholar]
  17. Palosaari H. , Parisien J. P. , Rodriguez J. J. , Ulane C. M. , Horvath C. M. . ( 2003; ). STAT protein interference and suppression of cytokine signal transduction by measles virus V protein. . J Virol 77:, 7635–7644. [CrossRef] [PubMed]
    [Google Scholar]
  18. Parks C. L. , Witko S. E. , Kotash C. , Lin S. L. , Sidhu M. S. , Udem S. A. . ( 2006; ). Role of V protein RNA binding in inhibition of measles virus minigenome replication. . Virology 348:, 96–106. [CrossRef] [PubMed]
    [Google Scholar]
  19. Radecke F. , Billeter M. A. . ( 1996; ). The nonstructural C protein is not essential for multiplication of Edmonston B strain measles virus in cultured cells. . Virology 217:, 418–421. [CrossRef] [PubMed]
    [Google Scholar]
  20. Radecke F. , Spielhofer P. , Schneider H. , Kaelin K. , Huber M. , Dötsch C. , Christiansen G. , Billeter M. A. . ( 1995; ). Rescue of measles viruses from cloned DNA. . EMBO J 14:, 5773–5784.[PubMed]
    [Google Scholar]
  21. Schneider H. , Kaelin K. , Billeter M. A. . ( 1997; ). Recombinant measles viruses defective for RNA editing and V protein synthesis are viable in cultured cells. . Virology 227:, 314–322. [CrossRef] [PubMed]
    [Google Scholar]
  22. Spehner D. , Drillien R. , Howley P. M. . ( 1997; ). The assembly of the measles virus nucleoprotein into nucleocapsid-like particles is modulated by the phosphoprotein. . Virology 232:, 260–268. [CrossRef] [PubMed]
    [Google Scholar]
  23. Takeuchi K. , Kadota S. I. , Takeda M. , Miyajima N. , Nagata K. . ( 2003; ). Measles virus V protein blocks interferon (IFN)-alpha/beta but not IFN-gamma signaling by inhibiting STAT1 and STAT2 phosphorylation. . FEBS Lett 545:, 177–182. [CrossRef] [PubMed]
    [Google Scholar]
  24. Tatsuo H. , Yanagi Y. . ( 2002; ). The morbillivirus receptor SLAM (CD150). . Microbiol Immunol 46:, 135–142.[PubMed] [CrossRef]
    [Google Scholar]
  25. Tober C. , Seufert M. , Schneider H. , Billeter M. A. , Johnston I. C. , Niewiesk S. , ter Meulen V. , Schneider-Schaulies S. . ( 1998; ). Expression of measles virus V protein is associated with pathogenicity and control of viral RNA synthesis. . J Virol 72:, 8124–8132.[PubMed]
    [Google Scholar]
  26. Volchkov V. E. , Volchkova V. A. , Muhlberger E. , Kolesnikova L. V. , Weik M. , Dolnik O. , Klenk H. D. . ( 2001; ). Recovery of infectious Ebola virus from complementary DNA: RNA editing of the GP gene and viral cytotoxicity. . Science 291:, 1965–1969. [CrossRef] [PubMed]
    [Google Scholar]
  27. von Messling V. , Springfeld C. , Devaux P. , Cattaneo R. . ( 2003; ). A ferret model of canine distemper virus virulence and immunosuppression. . J Virol 77:, 12579–12591. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/vir.0.050872-0
Loading
/content/journal/jgv/10.1099/vir.0.050872-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error