RT Journal Article SR Electronic(1) A1 Baron, Jana A1 Baron, MichaelYR 2013 T1 Creation of a completely helper cell-dependent recombinant morbillivirus JF Journal of General Virology, VO 94 IS 6 SP 1195 OP 1199 DO https://doi.org/10.1099/vir.0.050872-0 PB Microbiology Society, SN 1465-2099, AB We have created a completely helper cell-dependent morbillivirus by modifying the genome to remove the coding sequence of the phosphoprotein (P) and recovering the recombinant virus in a cell line constitutively expressing the P protein. The P protein-deleted virus (P−) grew very inefficiently unless both of the viral accessory proteins (V and C) were also expressed. Growth of the virus was restricted to the P-expressing cell line. The P− virus grew more slowly than the parental virus and expressed much less viral protein in infected cells. The technique could be used to create virus-like particles for use as a vaccine or as antigen in immunological or serological assays., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/vir.0.050872-0