1887

Abstract

Infectious salmon anaemia virus (ISAV) is a new orthomyxovirus-like virus. Thirteen isolates of ISAV (11 from Canada, one from Norway and one from Scotland) were studied for their replication in the CHSE-214 cell line compared with that in the SHK-1 cell line. All isolates replicated in SHK-1 cells, producing CPE between 3 and 12 days post-inoculation (p.i.). Six Canadian isolates also replicated in CHSE-214 cells, with production of CPE between 4 and 17 days p.i. Analysis of a one-step growth curve of ISAV in CHSE-214 cells showed that progeny virions remained predominantly cell-associated, accounting for the focalized nature of the CPE in the cell monolayer. One isolate (HKS 36) replicated in CHSE-214 cells, as shown by positive RT–PCR results of blind passages, but was non-cytopathic. All of the isolates were analysed for genetic heterogeneity by RT–PCR and RFLP with RI and I in a fraction of genome segment 2. The Canadian isolates showed a different RFLP profile to those of isolates Glesvaer/2/90 from Norway and 390/98 from Scotland. Structural proteins of four isolates, ‘Back Bay 98’, RPC/NB-877, RPC/NB-049 and Glesvaer/2/90, were examined further by SDS–PAGE. All viruses showed four major polypeptides, designated here as VP1–VP4, in Coomassie blue-stained gels. In isolates Glesvaer/2/90 and RPC/NB-877, these viral proteins had estimated molecular masses of 74, 53, 46 and 26·5 kDa, respectively. Viral proteins in isolates ‘Back Bay 98’ and RPC/NB-049 were of similar sizes, except that VP3 was 43 kDa. Taken together, these results show that there are phenotypic differences among strains of ISAV.

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2000-01-01
2020-09-23
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References

  1. Blake, S., Bouchard, D., Keleher, W., Opitz, M. & Nicholson, B. L. ( 1999; ). Genomic relationships of the North American isolate of infectious salmon anemia virus (ISAV) to the Norwegian strain of ISAV. Diseases of Aquatic Organisms 35, 139-144.[CrossRef]
    [Google Scholar]
  2. Bouchard, D., Keleher, W., Opitz, H. M., Blake, S., Edwards, K. C. & Nicholson, B. L. ( 1999; ). Isolation of infectious salmon anemia virus (ISAV) from Atlantic salmon in New Brunswick, Canada. Diseases of Aquatic Organisms 35, 131-137.[CrossRef]
    [Google Scholar]
  3. Brenner, S. & Horne, R. W. ( 1959; ). A negative staining method for high resolution electron microscopy of viruses. Biochimica et Biophysica Acta 34, 103-110.[CrossRef]
    [Google Scholar]
  4. Byrne, P. J., MacPhee, D. D., Ostland, V. E., Johnson, G. & Ferguson, H. W. ( 1998; ). Haemorrhagic kidney syndrome of Atlantic salmon, Salmo salar L. Journal of Fish Diseases 21, 81-91.[CrossRef]
    [Google Scholar]
  5. Dannevig, B. H., Falk, K. & Krogsrud, J. ( 1993; ). Leucocytes from Atlantic salmon, Salmo salar L., experimentally infected with infectious salmon anaemia (ISA) exhibit an impaired response to mitogens. Journal of Fish Diseases 16, 351-359.[CrossRef]
    [Google Scholar]
  6. Dannevig, B. H., Falk, K. & Namork, E. ( 1995; ). Isolation of the causal virus of infectious salmon anaemia (ISA) in a long-term cell line from Atlantic salmon head kidney. Journal of General Virology 76, 1353-1359.[CrossRef]
    [Google Scholar]
  7. Evensen, Ø., Thorud, K. E. & Olsen, Y. A. ( 1991; ). A morphological study of the gross and light microscopic lesions of infectious anaemia in Atlantic salmon (Salmo salar). Research in Veterinary Science 51, 215-222.[CrossRef]
    [Google Scholar]
  8. Falk, K., Namork, E., Rimstad, E., Mjaaland, S. & Dannevig, B. H. ( 1997; ). Characterization of infectious salmon anemia virus, an orthomyxo-like virus isolated from Atlantic salmon (Salmo salar L.). Journal of Virology 71, 9016-9023.
    [Google Scholar]
  9. Fryer, J. L., Yusha, A. & Pilcher, K. S. ( 1965; ). The in vitro cultivation of tissue and cells of Pacific salmon and steelhead trout. Annals of the New York Academy of Sciences 126, 566-586.
    [Google Scholar]
  10. Ilobi, C. P., Nicolson, C., Taylor, J., Mumford, J. A., Wood, J. M. & Robertson, J. S. ( 1998; ). Direct sequencing of the HA gene of clinical equine H3N8 influenza virus and comparison with laboratory derived viruses. Archives of Virology 143, 891-901.[CrossRef]
    [Google Scholar]
  11. Kendal, A. P. ( 1975; ). A comparison of ‘influenza C’ with prototype myxoviruses: receptor-destroying activity (neuraminidase) and structural polypeptides. Virology 65, 87-99.[CrossRef]
    [Google Scholar]
  12. Krossoy, B., Hordvik, I., Nilsen, F., Nylund, A. & Endresen, C. ( 1999; ). The putative polymerase sequence of infectious salmon anemia virus suggests a new genus within the Orthomyxoviridae. Journal of Virology 73, 2136-2142.
    [Google Scholar]
  13. Laemmli, U. K. ( 1970; ). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.[CrossRef]
    [Google Scholar]
  14. Lazarowitz, S. G. & Choppin, P. W. ( 1975; ). Enhancement of the infectivity of influenza A and B viruses by proteolytic cleavage of the hemagglutinin polypeptide. Virology 68, 440-454.[CrossRef]
    [Google Scholar]
  15. Lazarowitz, S. G., Goldberg, A. R. & Choppin, P. W. ( 1973; ). Proteolytic cleavage by plasmin of the HA polypeptide of influenza virus: host cell activation of serum plasminogen. Virology 56, 172-180.[CrossRef]
    [Google Scholar]
  16. Mjaaland, S., Rimstad, E., Falk, K. & Dannevig, B. H. ( 1997; ). Genomic characterization of the virus causing infectious salmon anemia in Atlantic salmon (Salmo salar L.): an orthomyxo-like virus in a teleost. Journal of Virology 71, 7681-7686.
    [Google Scholar]
  17. Mullins, J. E., Groman, D. & Wadwoska, D. ( 1998; ). Infectious salmon anaemia in salt water Atlantic salmon (Salmo salar L.) in New Brunswick, Canada. Bulletin of the European Association of Fish Pathologists 11, 110-114.
    [Google Scholar]
  18. Nylund, A., Alexandersen, S., Løvik, P. & Jakobsen, P. ( 1994; ). The response of brown trout (Salmo trutta L.) to repeated challenge with infectious salmon anaemia (ISA). Bulletin of the European Association of Fish Pathologists 14, 167-170.
    [Google Scholar]
  19. Nylund, A., Krossøy, B., Devold, M., Aspehaug, V., Steine, N. O. & Hovland, T. ( 1999; ). Outbreak of ISA during first feeding of salmon fry (Salmo salar). Bulletin of the European Association of Fish Pathologists 19, 70-74.
    [Google Scholar]
  20. Reed, L. J. & Muench, H. ( 1938; ). A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27, 493-497.
    [Google Scholar]
  21. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  22. Thorud, K. & Djupvik, H. O. ( 1988; ). Infectious anaemia in Atlantic salmon (Salmo salar L.). Bulletin of the European Association of Fish Pathologists 18, 109-111.
    [Google Scholar]
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