1887

Abstract

The mechanisms were investigated of haematopoietic progenitor growth inhibition, observed after infection of cord blood mononuclear cells (CBMNC) by a clinical isolate of dengue 3 (29–56DSS). The level of virus replication was not different when CBMNC were inoculated with 29–56DSS compared with a prototype strain of dengue 3 (H-87) which had no inhibitory effect. An inhibitory effect was also observed when cell-free and heat-inactivated supernatants from 29–56DSS cultures, but not from H-87 cultures, were added to cultures of normal CBMNC, suggesting an indirect mechanism via the release of soluble suppressive factor(s). Macrophage inflammatory protein-1a (MIP-1α ) was detected at a significantly higher level in 29–56DSS cultures than in controls. Blocking experiments with anti-MIP-1α antibody demonstrated that the inhibitory effect was related at least partly to high MIP-1α levels. To our knowledge, this is the first report suggesting an indirect effect of dengue infection on haematopoiesis mediated by a suppressive cytokine.

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/content/journal/jgv/10.1099/0022-1317-79-8-1889
1998-08-01
2022-05-23
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References

  1. Anderson R., Wang S., Osiowy C., Issekutz A. 1997; Activation of endothelial cells via antibody-enhanced dengue virus infection of peripheral blood monocytes. Journal of Virology 71:4226–4232
    [Google Scholar]
  2. Bierman H. R., Nelson E. R. 1965; Hematodepressive virus diseases of Thailand. Annals of Internal Medicine 62:867–884
    [Google Scholar]
  3. Bonnet D., Lemoine F. M., Najman A., Guigon M. 1995; Comparison of the inhibitory effect of AcSDKP, TNF-α, TGF-β and MIP-1α on marrow-purified CD34+ progenitors. Experimental Hematology 23:551–556
    [Google Scholar]
  4. Canque B., Rosenzwajg M., Gey A., Tartour E., Fridman W. H., Gluckman J. C. 1996; Macrophage inflammatory protein-1α induced by human immunodeficiency virus infection of monocyte-derived macrophages. Blood 5:2011–2019
    [Google Scholar]
  5. Chomczynski P., Sacchi N. 1987; Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Analytical Biochemistry 162:156–159
    [Google Scholar]
  6. Chungue E., Roche C., Lefevre M. F., Barbazan P., Chanteau S. 1993; Ultra-rapid, simple, sensitive, and economical silica method for extraction of dengue viral RNA from silica specimens and mosquitoes by reverse transcriptase-polymerase chain reaction. Journal of Medical Virology 40:142–145
    [Google Scholar]
  7. Graham G. J., Wright E. G., Hewick R., Wolpe S. D., Wilkie N. M., Donaldson D., Loimore S., Pragnell I. B. 1990; Identification and characterization of an inhibitor of haematopoietic stem cell proliferation. Nature 344:442–444
    [Google Scholar]
  8. Guigon M., Bonnet D., Lemoine F., Kobari L., Parmentier C., Mary J. Y., Najman A. 1990; Inhibition of human bone marrow progenitors by the synthetic tetrapeptide AcSDKP. Experimental Hematology 18:112–115
    [Google Scholar]
  9. Halstead S. B. 1982; Dengue: hematologic aspects. Seminars in Hematology 19:116–131
    [Google Scholar]
  10. Hober D., Poli L., Roblin B., Gestas P., Chungue E., Granic G., Imbert P., Pecarere J. L., Vergez-Pascal R., Wattre P., Maniez-Montreuil M. 1993; Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β in dengue-infected patients. American Journal of Tropical Medicine and Hygiene 48:324–331
    [Google Scholar]
  11. Igarashi A., Mantani M. 1974; Rapid titration of dengue virus type 4 infectivity by counting fluorescent foci. Biken Journal 17:87–93
    [Google Scholar]
  12. Lanciotti R. S., Calisher C. H., Gubler D. J., Chang G., Vordam A. V. 1992; Rapid detection and typing of clinical samples using reverse transcriptase-polymerase chain reaction. Journal of Clinical Microbiology 30:545–551
    [Google Scholar]
  13. Murgue B., Cassar O., Guigon M., Chungue E. 1997; Dengue virus inhibits human hematopoietic progenitor growth in vitro . Journal of Infectious Diseases 175:1497–1501
    [Google Scholar]
  14. Nelson E. R., Bierman H. R. 1964; Dengue fever: a thrombocytopenic disease?. Journal of the American Medical Association 190:99–103
    [Google Scholar]
  15. Yadav M., Kamath K. R., Iyngkaran N., Sinniah M. 1991; Dengue haemorrhagic fever and dengue shock syndrome : are they tumor necrosis factor-mediated disorders?. FEMS Microbiology and Immunology 89:45–50
    [Google Scholar]
  16. Zauli G., Vitale M., Gibellini D., Capitani S. 1996; Inhibition of purified CD34+ hematopoietic progenitor cells by human immuno-deficiency virus 1 or gp 120 mediated by endogenous transforming growth factor β1. Journal of Experimental Medicine 183:99–108
    [Google Scholar]
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