@article{mbs:/content/journal/jgv/10.1099/0022-1317-79-8-1889, author = "Murgue, Bernadette and Cassar, Olivier and Deparis, Xavier and Guigon, Martine and Chungue, Eliane", title = "Implication of macrophage inflammatory protein-1α in the inhibition of human haematopoietic progenitor growth by dengue virus.", journal= "Journal of General Virology", year = "1998", volume = "79", number = "8", pages = "1889-1893", doi = "https://doi.org/10.1099/0022-1317-79-8-1889", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-8-1889", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", abstract = "The mechanisms were investigated of haematopoietic progenitor growth inhibition, observed after in vitro infection of cord blood mononuclear cells (CBMNC) by a clinical isolate of dengue 3 (29–56DSS). The level of virus replication was not different when CBMNC were inoculated with 29–56DSS compared with a prototype strain of dengue 3 (H-87) which had no inhibitory effect. An inhibitory effect was also observed when cell-free and heat-inactivated supernatants from 29–56DSS cultures, but not from H-87 cultures, were added to cultures of normal CBMNC, suggesting an indirect mechanism via the release of soluble suppressive factor(s). Macrophage inflammatory protein-1a (MIP-1α ) was detected at a significantly higher level in 29–56DSS cultures than in controls. Blocking experiments with anti-MIP-1α antibody demonstrated that the inhibitory effect was related at least partly to high MIP-1α levels. To our knowledge, this is the first report suggesting an indirect effect of dengue infection on haematopoiesis mediated by a suppressive cytokine.", }