RT Journal Article SR Electronic(1) A1 Murgue, Bernadette A1 Cassar, Olivier A1 Deparis, Xavier A1 Guigon, Martine A1 Chungue, ElianeYR 1998 T1 Implication of macrophage inflammatory protein-1α in the inhibition of human haematopoietic progenitor growth by dengue virus. JF Journal of General Virology, VO 79 IS 8 SP 1889 OP 1893 DO https://doi.org/10.1099/0022-1317-79-8-1889 PB Microbiology Society, SN 1465-2099, AB The mechanisms were investigated of haematopoietic progenitor growth inhibition, observed after in vitro infection of cord blood mononuclear cells (CBMNC) by a clinical isolate of dengue 3 (29–56DSS). The level of virus replication was not different when CBMNC were inoculated with 29–56DSS compared with a prototype strain of dengue 3 (H-87) which had no inhibitory effect. An inhibitory effect was also observed when cell-free and heat-inactivated supernatants from 29–56DSS cultures, but not from H-87 cultures, were added to cultures of normal CBMNC, suggesting an indirect mechanism via the release of soluble suppressive factor(s). Macrophage inflammatory protein-1a (MIP-1α ) was detected at a significantly higher level in 29–56DSS cultures than in controls. Blocking experiments with anti-MIP-1α antibody demonstrated that the inhibitory effect was related at least partly to high MIP-1α levels. To our knowledge, this is the first report suggesting an indirect effect of dengue infection on haematopoiesis mediated by a suppressive cytokine., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-79-8-1889