1887

Abstract

The viral lymphoproliferative response of pigs vaccinated against foot-and-mouth disease virus (FMDV) has been characterized. Peripheral blood mononuclear cells from immunized animals up to 1 year post-immunization (p.i.) showed a time-dependent FMDV-specific response, as assayed by virus-specific cellular blastogenesis. The optimum viral concentration decreased with time (around 20 weeks p.i.), and the response was faster and weaker. Lymphoproliferation appeared to be mainly due to CD4 T cells. The response was heterotypic, being induced by all FMDV serotypes tested (C, A and O) after only two vaccinations with FMDV of serotype C (C-S8). Each individual structural protein assessed (VP1, VP2 and VP3) induced proliferation, with VP3 and VP1 being more effective stimulators. serum neutralization activity and FMDV-specific IgG production were found to be active even at 1 year p.i.

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1992-10-01
2021-10-26
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References

  1. Abou-Zeid C., Filley E., Steele J., Rook G. A. W. 1987; A simple method for using antigens separated by polyacrylamide gel electrophoresis to stimulate lymphocytes in vitro after converting bands cut from Western blots into antigen-bearing particles. Journal of Immunological Methods 98:5–10
    [Google Scholar]
  2. Babin M., Hernandez C., Sanchez C., Dominguez J. 1991; Obtencion de anticuerpos monoclonales frente al virus IPN: su utilización para el dignóstico por medio de la inmunofluorecencia. Medicina Veterinaria 8:85–90
    [Google Scholar]
  3. Bachrach H. L. 1977; Foot-and-mouth disease virus: properties, molecular biology and immunogenicity. Beltsville Symposia on Agricultural Research 1:3–22
    [Google Scholar]
  4. Bachrach H. L., Moore D. M., McKercher P. D., Polatnick J. 1975; Immune and antibody response to an isolated capsid protein of foot-and-mouth disease virus. Journal of Immunology 115:1636–1641
    [Google Scholar]
  5. Bahnemann H. G. 1974; Binary ethylenimine as inactivant for foot-and-mouth disease virus and its application for vaccine production. Archives of Virology 47:45–56
    [Google Scholar]
  6. Barnett P. V., Ouldridge E. J., Rowlands D. J., Brown F., Parry N. R. 1989; Neutralizing epitopes of type O foot-and-mouth disease virus. I. Identification and characterization of three functionally independent, conformational sites. Journal of General Virology 70:1483–1491
    [Google Scholar]
  7. Barteling S. J., Vreeswuk J. 1991; Developments in foot-and-mouth disease vaccines. Vaccine 9:75–88
    [Google Scholar]
  8. Baxt B., Vakharia V., Moore D. M., Franks A. J., Morgan D. O. 1989; Analysis of neutralizing antigenic sites on the surface of type A12 foot-and-mouth disease virus. Journal of Virology 63:2143–2151
    [Google Scholar]
  9. Beck E., Strohmaier K. 1987; Subtyping of European foot-and-mouth disease virus strains by nucleotide sequence determination. Journal of Virology 61:1621–1629
    [Google Scholar]
  10. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against foot-and-mouth disease virus by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature, London 298:30–33
    [Google Scholar]
  11. Boyer M., Novak Z., Fotedar A., Fraga E., Singh B. 1990; Critical role of an amino acid residue in a T cell determinant is due to its interaction with neighbouring non-critical residues. European Journal of Immunology 20:2145–2148
    [Google Scholar]
  12. Broekhuijsen M. P., van Rijn J. M. M., Blom A. J. M., Pouwels P. H., Enger-Valk B. E., Brown F., Francis M. J. 1987; Fusion proteins with multiple copies of the major antigenic determinant of foot-and-mouth disease virus protect both the natural host and laboratory animals. Journal of General Virology 68:3137–3143
    [Google Scholar]
  13. Brown F. 1989; The next generation of foot-and-mouth disease vaccines. In Synthetic Peptides: Approaches to Biological Problems pp. 127–142 Edited by Tam J. P., Kaiser E. T. New York: Alan R. Liss;
    [Google Scholar]
  14. Carrillo C., Dopazo J., Moya A., Gonzalez M., Martinez M. A., Sáiz I. C., Sobrino F. 1989; Comparison of vaccine strains and the virus causing the 1986 foot-and-mouth disease outbreak in Spain: epizootiological analysis. Virus Research 15:45–56
    [Google Scholar]
  15. Clarke B. E., Newton S. E., Carroll A. R., Francis M. J., Appleyard G., Syred A. D., Highfield P. E., Rowlands D. J., Brown F. 1987; Improved immunogenicity of a peptide after fusion to hepatitis B core protein. Nature, London 330:381–384
    [Google Scholar]
  16. Collen T., Doel T. R. 1990; Heterotypic recognition of foot-and-mouth disease virus by cattle lymphocytes. Journal of General Virology 71:309–315
    [Google Scholar]
  17. Collen T., Doel T. R. 1991; Analysis of specificity of T-cells reactive with foot-and-mouth disease (FMD) virus suggests that B cell presentation influences the memory repertoire in cattle. VIIth Meeting of the European Study Group on Picomaviruses (Europic 91), Canterbury, U.K..
    [Google Scholar]
  18. Collen T., Pullen L., Doel T. R. 1989; T cell-dependent induction of antibody against foot-and-mouth disease virus in a mouse model. Journal of General Virology 70:395–403
    [Google Scholar]
  19. Collen T., DiMarchi R., Doel T. R. 1991; A T cell epitope in VP1 of foot-and-mouth disease virus is immunodominant for vaccinated cattle. Journal of Immunology 146:749–755
    [Google Scholar]
  20. DiMarchi R., Brooke G., Gale C., Cracknell V., Doel T., Mowat N. 1986; Protection of cattle against foot-and-mouth disease by a synthetic peptide. Science 232:639–641
    [Google Scholar]
  21. Domingo E., Mateu M. G., Martinez M. A., Dopazo J., Moya A., Sobrino F. 1990; Genetic variability and antigenic diversity of foot-and-mouth disease virus. In Applied Virology Research vol II pp. 233–266 Edited by Kurstark K. G., Marusyk F. A., Murphy A., Van Regenmortel M. H. V. New York: Plenum Publishing;
    [Google Scholar]
  22. Francis M. J., Fry C. M., Rowlands D. J., Brown F., Bittle J. L., Houghten R. A., Lerner R. A. 1985; Immunological priming with synthetic peptides of foot-and-mouth disease virus. Journal of General Virology 66:2347–2354
    [Google Scholar]
  23. Francis M. J., Fry C. M., Rowlands D. J., Bittle J. L., Houghten R. A., Lerner R. A. 1987; Immune response to uncoupled peptides of foot-and-mouth disease virus. Immunology 61:1–6
    [Google Scholar]
  24. González S., Mendoza C., Sanchez Vizcaino J. M., Alonso F. 1990; Inhibitory effect of African swine fever virus on lectin-dependent swine lymphocyte proliferation. Veterinary Immunology and Immunopathology 26:71–80
    [Google Scholar]
  25. Hammerberg C., Schurig G. 1986; Characterization of monoclonal antibodies directed against swine leucocytes. Veterinary Immunology and Immunopathology 11:107–121
    [Google Scholar]
  26. King A. M. Q., Underwood B. O., McCahon D., Newman J. W., Brown F. 1981; Biochemical identification of viruses causing the 1981 outbreak of foot-and-mouth disease in the U.K. Nature, London 293:479–480
    [Google Scholar]
  27. Kitson J. D. A., McCahon D., Belsham G. J. 1990; Sequence analysis of monoclonal antibody resistant mutants of type O foot-and-mouth disease virus: evidence for the involvement of the three surface exposed capsid proteins in four antigenic sites. Virology 179:26–34
    [Google Scholar]
  28. Kleid D. G., Yansura D. G., Small B., Dowbenko D. J., Moore D. M., Grubman M. J., McKercher P. D., Morgan D. O., Robertson B. H., Bachrach H. L. 1981; Cloned viral protein vaccine for foot-and-mouth disease: response in cattle and swine. Science 214:1125–1129
    [Google Scholar]
  29. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  30. Laporte J., Grosclaude J., Wantyghem J., Bernards S., Rooze P. 1973; Neutralisation en culture cellulaire infectieux du virus de la fièvre aphteuse par des sèrums provenant de porcs immunisès a l’aide d’une protèine virale purifiée. Comptes Rendus de l’Academie des Sciences 276D:3399–3401
    [Google Scholar]
  31. Mateu M. G., Rocha E., Vicente O., Vayreda F., Navalpotro C., Andreu D., Pedroso E., Giralt E., Enjuanes L., Domingo E. 1987; Reactivity with monoclonal antibodies of viruses from an episode of foot-and-mouth disease virus. Virus Research 8:261–274
    [Google Scholar]
  32. Mulcahy G., Gale C., Robertson P., Iysian S., DiMarchi R. D., Doel T. R. 1990; Isotype response of infected, virus vaccinated and peptide vaccinated cattle to foot-and-mouth disease virus. Vaccine 8:249–256
    [Google Scholar]
  33. Pescovitz M. D., Lunney J., Sachs D. 1984; Preparation and characterization of monoclonal antibodies reactive with porcine PBL. Journal of Immunology 133:368–375
    [Google Scholar]
  34. Rueckert R. R. 1990; Picomaviruses and their replication. In Virology 2nd edn, pp. 507–548 Edited by Fields B. N., Knipe D. M. New York: Raven Press;
    [Google Scholar]
  35. Sachs D., Leight G., Cone J., Schwarz S., Stuart L., Rosenberg S. 1976; Transplantation in miniature swine. I. Fixation of the major histocompatibility complex. Transplantation 22:559–567
    [Google Scholar]
  36. Sáiz J. C., Gonzalez M. J., Morgan D. O., Card J. L., Sobrino F., Moore D. M. 1989; Antigenic comparison of different foot-and-mouth disease virus types using monoclonal antibodies defining multiple neutralizing epitopes on FMDV A5 subtypes. Virus Research 13:45–60
    [Google Scholar]
  37. Sáiz J. C., Gonzalez M. J., Borca M. V., Sobrino F., Moore D. M. 1991; Identification of neutralizing antigenic sites on VP1 and VP2 of type A5 foot-and-mouth disease virus, defined by neutralization resistant variants. Journal of Virology 65:2518–2524
    [Google Scholar]
  38. Stave J. W., Card J. L., Morgan D. O. 1986; Analysis of foot-and-mouth disease virus type O1 Brugge neutralization epitopes using monoclonal antibodies. Journal of General Virology 67:2083–2092
    [Google Scholar]
  39. Strebel K., Beck E., Strohmaier R. R., Schaller H. 1986; Characterization of foot-and-mouth disease gene products with antisera against bacterially synthesized fusion proteins. Journal of Virology 57:983–991
    [Google Scholar]
  40. Strohmaier K., Franze R., Adan K. H. 1982; Location and characterization of the antigenic portion of the FMDV immunizing protein. Journal of General Virology 59:295–306
    [Google Scholar]
  41. Trautman R., Harris W. F. 1977; Modelling and computer simulation approach to the mechanisms of foot-and-mouth disease virus neutralization assays. Scandinavian Journal of Immunology 6:831–841
    [Google Scholar]
  42. Wardley R. C., Chapman W. G., Garland A. J. M. 1979; A blastogenic test for foot-and-mouth disease. Journal of Hygiene 83:507–512
    [Google Scholar]
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