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Journal of General Virology header logo Journal of General Virology

Volume 67, Issue 11

Selection and Characterization of an Interferon-responsive Clonal Cell Line of HeLa Cells No Access

Abstract

Summary

HeLa cells generally do not respond well to interferon (IFN). We have used -1, an IFN-sensitive mutant of mengovirus, to select a clone of IFN-responsive HeLa cells (F-H12). At moderate levels of human α/β IFN, -1 yields were fivefold lower in these cells than in similarly protected control cells. In contrast, wild-type mengovirus, vesicular stomatitis virus and a wild-type and thymidine kinase-negative strains of herpes simplex virus type 1 grew equally well in both cell lines. By a cell survival assay, the F-H12 line was up to 100 times more responsive to IFN than the parental line when challenged by -1. 2′-5′-Oligo(A)-dependent endonuclease activity was the same in both lines. These observations cannot be accounted for by enhanced induction of IFN following infection.

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Keyword(s): HeLa cells , interferon-responsive and mengovirus
© The Journal of General Virology 1986
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1986-11-01
2025-12-10

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References

  1. Adams A., Strander H., Cantell K. 1975; Sensitivity of the Epstein-Barr virus transformed human lymphoid cell lines to interferon. Journal of General Virology 28:207–217
     [Google Scholar]
  2. Affabris E., Jemma C., Rossi G. B. 1982; Isolation of interferon-resistant variants of Friend erythroleukemia cells: effects of interferon and ouabain. Virology 120:441–452
     [Google Scholar]
  3. Baglioni C., Branca A. A., D’alessandro S. B., Hossenlopp D., Chadra K. C. 1982; Low interferon binding activity of two human cell lines which respond poorly to the antiviral and antiproliferative activity of interferon. Virology 122:202–206
     [Google Scholar]
  4. Cayley P. J., Knight M., Kerr I. M. 1982; Virus mediated inhibition of the ppp (A2′p)nA system and its prevention by interferon. Biochemical and Biophysical Research Communications 104:376–382
     [Google Scholar]
  5. Chany C., Vignal M. 1970; Effect of prolonged interferon treatment on mouse embryonic fibroblasts transformed by murine sarcoma virus. Journal of General Virology 7:203–210
     [Google Scholar]
  6. Chebath J., Merlin G., Metz R., Benech P., Revel M. 1983; Interferon-induced 56, 000 Mr protein and its mRNA in human cells: molecular cloning and partial sequence of the cDNA. Nucleic Acids Research 11:1213–1226
     [Google Scholar]
  7. Czarniecki C. W., Allen P. T. 1984; Disparate response of encephalomyocarditis virus and MM virus to interferon in JLS-V9R cells. Antiviral Research 4:351–355
     [Google Scholar]
  8. Daher K. A., Samuel C. E. 1982; Mechanism of interferon action: differential effect of interferon on the synthesis of simian virus 40 and reovirus polypeptides in monkey kidney cells. Virology 117:379–390
     [Google Scholar]
  9. Declercq E. D. A. 1982; Interferon: a molecule for all seasons. In Virus Infections pp 87–138 Edited by Olson L. C. New York: Marcel Dekker;
     [Google Scholar]
  10. Dron M., Tovey M. G. 1983; Isolation of Daudi cells with reduced sensitivity to interferon. I. Characterization. Journal of General Virology 64:2641–2647
     [Google Scholar]
  11. Dron M., Tovey M. G., Eid P. 1985; Isolation of Daudi cells with reduced sensitivity to interferon. III. Interferon-induced proteins in relation to the phenotype of interferon resistance. Journal of General Virology 66:787–795
     [Google Scholar]
  12. Fleischmann W. R. Jr, Simon E. H. 1973; Effect of interferon on virus production from isolated single cells. Journal of General Virology 20:127–137
     [Google Scholar]
  13. Fout G. S., Simon E. H. 1981; Studies on an interferon-sensitive mutant of mengovirus: effects on host RNA and protein synthesis. Journal of General Virology 52:391–394
     [Google Scholar]
  14. Fout G. S., Simon E. H. 1983; Antiviral activities directed against wild-type and interferon-sensitive mengovirus. Journal of General Virology 64:1543–1555
     [Google Scholar]
  15. Gresser I., Bandu M.-T., Brouty-Boye D. 1974; Interferon and cell division. IX. Interferon-resistant L1210 cells: characteristics and origin. Journal of the National Cancer Institute 52:553–559
     [Google Scholar]
  16. Knight E. Jr, Korant B. D. 1979; Fibroblast interferon induces synthesis of four proteins in human fibroblast cells. Proceedings of the National Academy of Sciences, U.S.A. 76:1824–1827
     [Google Scholar]
  17. Kuwata T., Fuse A., Morinaga N. 1976; Effects of interferon on cell and virus growth in transformed human cell lines. Journal of General Virology 33:7–15
     [Google Scholar]
  18. Mcmahon M., Kerr I. M. 1983; The biochemistry of the antiviral state. In Interferons: From Molecular Biology to Clinical Application pp 89–108 Edited by Burke D. C., Morris A. G. Cambridge: Cambridge University Press;
     [Google Scholar]
  19. Marcus P. L., Guidon P. T. Jr, Sekellick M. J. 1981; Interferon induction by viruses. VII. Mengovirus: ‘interferon-sensitive’ mutant phenotype attributed to interferon-inducing particle activity. Journal of Interferon Research 1:601–611
     [Google Scholar]
  20. Revel M. 1984; The interferon system in man: nature of the interferon molecules and mode of action. In Antiviral Drugs and Interferon: The Molecular Basis of Their Activity pp 357–438 Edited by Becker Y. Boston: Martinus Nijhoff;
     [Google Scholar]
  21. Rice A. P., Duncan R., Hershey J. W. B., Kerr I. M. 1985; Double-stranded RNA-dependent protein kinase and 2-5A system are both activated in interferon-treated, encephalomyocarditis virus-infected HeLa cells. Journal of Virology 54:894–898
     [Google Scholar]
  22. Rubin B. Y., Gupta S. L. 1980; Interferon-induced proteins in human fibroblasts and development of the antiviral state. Journal of Virology 34:446–454
     [Google Scholar]
  23. Samuel C. E., Duncan R., Knutson G. S., Hershey J. W. B. 1984; Mechanism of interferon action: increased phosphorylation of protein synthesis initiation factor eIF-2α in interferon-treated, reovirus-infected mouse L929 fibroblastsin vitro andin vivo. Journal of Biological Chemistry 259:13451–13457
     [Google Scholar]
  24. Seabright M. 1971; A rapid banding technique for human chromosomes. Lancet ii:971–972
     [Google Scholar]
  25. Sen G. C., Herz R. E. 1983; Differential antiviral effects of interferon in three murine cell lines. Journal of Virology 45:1017–1027
     [Google Scholar]
  26. Silverman R. H., Cayley P. J., Knight M., Gilbert C. S., Kerr I. M. 1982; Control of the ppp(A2′p)n-A system in HeLa cells. European Journal of Biochemistry 124:131–138
     [Google Scholar]
  27. Simon E. H., Kung S., Koh T. T., Brandman P. 1976; Interferon-sensitive mutants of mengovirus. I. Isolation and biological characterization. Virology 69:727–736
     [Google Scholar]
  28. Simon E. H., Isono N., Bradley C. 1984; The role of interferon induction in the interferon sensitivity of the mengovirus mutant,is- 1. Journal of General Virology 65:247–250
     [Google Scholar]
  29. Weissenbach J., Zeevi M., Landau T., Revel M. 1979; Identification of the translation products of human fibroblast interferon mRNA in reticulocyte lysates. European Journal of Biochemistry 98:1–8
     [Google Scholar]
  30. Whitaker-Dowling P., Youngner J. S. 1984; Characterization of a specific kinase inhibitory factor produced by vaccinia virus which inhibits the interferon-induced protein kinase. Virology 137:171–181
     [Google Scholar]
  31. Whitaker-Dowling P., Youngner J. S. 1986; Antiviral effects of interferon in different virus-host cell systems. In Mechanisms of Interferon Actions Edited by Pfeifer L. M. Boca Raton: CRC Press; (in press)
    [Google Scholar]
  32. Wreschner D. H., James T. C., Silverman R. H., Kerr I. M. 1981; Ribosomal RNA cleavage, nuclease activation and 2-5A (ppp(A2′p)nA) in interferon-treated cells. Nucleic Acids Research 91571–1581
     [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-67-11-2355
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Selection and Characterization of an Interferon-responsive Clonal Cell Line of HeLa Cells
J. Gen. Virol. 67, 2355 (1986); https://doi.org/10.1099/0022-1317-67-11-2355
/content/journal/jgv/10.1099/0022-1317-67-11-2355
/content/journal/jgv/10.1099/0022-1317-67-11-2355
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