@article{mbs:/content/journal/jgv/10.1099/0022-1317-67-11-2355, author = "Sakuragi, Anne L. Z. and Simon, Edward H.", title = "Selection and Characterization of an Interferon-responsive Clonal Cell Line of HeLa Cells", journal= "Journal of General Virology", year = "1986", volume = "67", number = "11", pages = "2355-2364", doi = "https://doi.org/10.1099/0022-1317-67-11-2355", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-67-11-2355", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "HeLa cells", keywords = "mengovirus", keywords = "interferon-responsive", abstract = "Summary HeLa cells generally do not respond well to interferon (IFN). We have used is-1, an IFN-sensitive mutant of mengovirus, to select a clone of IFN-responsive HeLa cells (F-H12). At moderate levels of human α/β IFN, is-1 yields were fivefold lower in these cells than in similarly protected control cells. In contrast, wild-type mengovirus, vesicular stomatitis virus and a wild-type and thymidine kinase-negative strains of herpes simplex virus type 1 grew equally well in both cell lines. By a cell survival assay, the F-H12 line was up to 100 times more responsive to IFN than the parental line when challenged by is-1. 2′-5′-Oligo(A)-dependent endonuclease activity was the same in both lines. These observations cannot be accounted for by enhanced induction of IFN following infection.", }