1887

Abstract

Summary

Monocyte-enriched cultures of goat peripheral blood leukocytes were exposed to caprine arthritis-encephalitis virus (CAEV) at an m.o.i. of 8 TCID per cell as measured in goat synovial membrane cell cultures. At the time of infection, 90% of the adherent cells displayed characteristic macrophage markers of phagocytic activity and cytoplasmic non-specific esterase. A productive infection was established, with extracellular infectious virus titres reaching a maximum of 10 TCID per ml at day 4 post-infection. Synthesis of CAEV antigens was detected by direct immunofluorescence and it was shown that 85% of the adherent cells contained virus antigen by day 6 post-infection. A cytopathic effect, characterized by giant cell formation and cell death, developed concomitantly with increased virus replication.

Keyword(s): arthritis , CAEV , in vitro and monocytes
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-58-1-195
1982-01-01
2019-10-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-58-1-195
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error