RT Journal Article SR Electronic(1) A1 Klevjer-Anderson, Paula A1 Anderso, Lawrence W.YR 1982 T1 Caprine Arthritis-Encephalitis Virus Infection of Caprine Monocytes JF Journal of General Virology, VO 58 IS 1 SP 195 OP 198 DO https://doi.org/10.1099/0022-1317-58-1-195 PB Microbiology Society, SN 1465-2099, AB SUMMARY Monocyte-enriched cultures of goat peripheral blood leukocytes were exposed to caprine arthritis-encephalitis virus (CAEV) at an m.o.i. of 8 TCID50 per cell as measured in goat synovial membrane cell cultures. At the time of infection, 90% of the adherent cells displayed characteristic macrophage markers of phagocytic activity and cytoplasmic non-specific esterase. A productive infection was established, with extracellular infectious virus titres reaching a maximum of 105.2 TCID50 per ml at day 4 post-infection. Synthesis of CAEV antigens was detected by direct immunofluorescence and it was shown that 85% of the adherent cells contained virus antigen by day 6 post-infection. A cytopathic effect, characterized by giant cell formation and cell death, developed concomitantly with increased virus replication., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-58-1-195