%0 Journal Article %A Klevjer-Anderson, Paula %A Anderso, Lawrence W. %T Caprine Arthritis-Encephalitis Virus Infection of Caprine Monocytes %D 1982 %J Journal of General Virology, %V 58 %N 1 %P 195-198 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-58-1-195 %K monocytes %K in vitro %K CAEV %K arthritis %I Microbiology Society, %X SUMMARY Monocyte-enriched cultures of goat peripheral blood leukocytes were exposed to caprine arthritis-encephalitis virus (CAEV) at an m.o.i. of 8 TCID50 per cell as measured in goat synovial membrane cell cultures. At the time of infection, 90% of the adherent cells displayed characteristic macrophage markers of phagocytic activity and cytoplasmic non-specific esterase. A productive infection was established, with extracellular infectious virus titres reaching a maximum of 105.2 TCID50 per ml at day 4 post-infection. Synthesis of CAEV antigens was detected by direct immunofluorescence and it was shown that 85% of the adherent cells contained virus antigen by day 6 post-infection. A cytopathic effect, characterized by giant cell formation and cell death, developed concomitantly with increased virus replication. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-58-1-195